Mechanism of Site-Selective DNA Nicking by the Hydrodioxyl (Perhydroxyl) Radical

• Published in: Biochemistry (Easton) Vol. 35; no. 14; pp. 4578 - 4583
• Main Authors: Dix, Thomas A, Hess, Kathleen M, Medina, Melissa A, Sullivan, Robert W, Tilly, Shannon L, Webb, Thomas L. L
• Format: Journal Article
• Language: English
• Published: United States American Chemical Society 09.04.1996
• Subjects: Binding Sites; DNA - chemistry; DNA - drug effects; DNA Damage; Free Radicals - chemistry; Free Radicals - toxicity; Models, Chemical; Molecular Probes; Peroxides - chemistry; Peroxides - toxicity; Plasmids - chemistry; Plasmids - drug effects; Poly dA-dT - chemistry

In previous studies, the ability of the hydrodioxyl (perhydroxyl) radical [HOO•, the conjugate acid of superoxide (O2 •-)] to “nick” DNA under biomimetic conditions was demonstrated, and a sequence selectivity was observed. A background level of nonspecific nicking also was noted. This paper provide...