Barcode DNA-Mediated Signal Amplifying Strategy for Ultrasensitive Biomolecular Detection on Matrix-Assisted Laser Desorption Ionization Time of Flight (MALDI-TOF) Mass Spectrometry

We have devised a barcode DNA-mediated signal amplifying strategy for ultrasensitive biomolecular detection by utilizing matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS). As a model target, thrombin was first captured by specific aptamer15 functionalized on...

Full description

Saved in:
Bibliographic Details
Published inAnalytical chemistry (Washington) Vol. 89; no. 17; pp. 8966 - 8973
Main Authors Ahmad, Raheel, Jang, Hyowon, Batule, Bhagwan S, Park, Hyun Gyu
Format Journal Article
LanguageEnglish
Published United States American Chemical Society 05.09.2017
Subjects
Amplification
Aptamers
Beads
Biomolecules
Chemistry
Cyanides
Deoxyribonucleic acid
Desorption
DNA
Gold
Ionization
Magnetic separation
Mass spectrometry
Mass spectroscopy
Molecules
Nanoparticles
Potassium
Potassium cyanide
Spectroscopy
Strategy
Thrombin
Online AccessGet full text

Cover

Abstract We have devised a barcode DNA-mediated signal amplifying strategy for ultrasensitive biomolecular detection by utilizing matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS). As a model target, thrombin was first captured by specific aptamer15 functionalized on magnetic beads (MBs-apt15) and sandwiched through the simultaneous interaction with gold nanoparticles modified with another specific aptamer29 and barcode DNA molecules (apt29-AuNPs-bcDNAs). The sandwiched complex was collected by convenient magnetic separation and then treated with potassium cyanide (KCN) to dissolve the gold nanoparticles (AuNPs) and consequently release the barcode DNA molecules (bcDNAs), which were then again magnetically separated and analyzed by using MALDI-TOF MS. Under optimized conditions, this strategy revealed an excellent sensitivity with a limit of detection of 0.89 aM in a wide linear detection range from 0 aM to 0.1 nM and exhibited an acceptable recovery for thrombin detection in complex biological matrices. This signal amplifying strategy based on MALDI-TOF MS could greatly enable the ultrasensitive detection of various low abundant biomolecules.
AbstractList We have devised a barcode DNA-mediated signal amplifying strategy for ultrasensitive biomolecular detection by utilizing matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS). As a model target, thrombin was first captured by specific aptamer15 functionalized on magnetic beads (MBs-apt15) and sandwiched through the simultaneous interaction with gold nanoparticles modified with another specific aptamer29 and barcode DNA molecules (apt29-AuNPs-bcDNAs). The sandwiched complex was collected by convenient magnetic separation and then treated with potassium cyanide (KCN) to dissolve the gold nanoparticles (AuNPs) and consequently release the barcode DNA molecules (bcDNAs), which were then again magnetically separated and analyzed by using MALDI-TOF MS. Under optimized conditions, this strategy revealed an excellent sensitivity with a limit of detection of 0.89 aM in a wide linear detection range from 0 aM to 0.1 nM and exhibited an acceptable recovery for thrombin detection in complex biological matrices. This signal amplifying strategy based on MALDI-TOF MS could greatly enable the ultrasensitive detection of various low abundant biomolecules.
Author Jang, Hyowon
Batule, Bhagwan S
Park, Hyun Gyu
Ahmad, Raheel
AuthorAffiliation Department of Chemical and Biomolecular Engineering (BK 21+ program)
AuthorAffiliation_xml – name: Department of Chemical and Biomolecular Engineering (BK 21+ program)
Author_xml – sequence: 1
  givenname: Raheel
  surname: Ahmad
  fullname: Ahmad, Raheel
– sequence: 2
  givenname: Hyowon
  surname: Jang
  fullname: Jang, Hyowon
– sequence: 3
  givenname: Bhagwan S
  surname: Batule
  fullname: Batule, Bhagwan S
– sequence: 4
  givenname: Hyun Gyu
  orcidid: 0000-0001-9978-3890
  surname: Park
  fullname: Park, Hyun Gyu
  email: hgpark@kaist.ac.kr
BackLink https://www.ncbi.nlm.nih.gov/pubmed/28780857$$D View this record in MEDLINE/PubMed
BookMark eNp9kU9v0zAchi00xLrBN0DIEpdxSLGdxE6O2UpHpZYd2p0jx_ml85TEwXYQ5Xvx_easHUgckCz53_M-lvxeoLPe9IDQe0rmlDD6WSo3l71s1QN0c1ERmsbpKzSjKSMRzzJ2hmaEkDhigpBzdOHcIyGUEsrfoHOWiYxkqZih39fSKlMDXnwrog3UWnqo8VbvgxkX3dDq5qD7Pd56G272B9wYi-_bsHPQO-31D8DX2nSmBTW20uIFeFBemx6HsZHe6p9R4Zx2k3cdUhPijB2emZXp9S_5vNzpDrBp8LLV-wePrzbFerGKdnfLT0HjHN4OwWtNB94e3qLXjWwdvDvNl-h--WV38zVa392ubop1JGPBfVTTjFSc501T00ZSkVU0nKQEFFUJyJoxmjFCVMZjSgStYk4VB55CAkmaVCK-RFdH72DN9xGcLzvtFLSt7MGMrqQ547nIBeMB_fgP-mhGG35xopLwUpzHLFDJkVLWOGehKQerO2kPJSXlVGsZai1fai1PtYbYh5N8rDqo_4ReegwAOQJT_O_D_3M-AbI3tKY
CitedBy_id crossref_primary_10_1016_S1872_2040_20_60056_1
crossref_primary_10_1016_j_talanta_2021_122222
crossref_primary_10_1021_acsami_8b17051
crossref_primary_10_1021_acs_chemrev_8b00136
crossref_primary_10_1021_acs_nanolett_2c04690
crossref_primary_10_1021_acssensors_1c01394
crossref_primary_10_1039_C8CC06934D
crossref_primary_10_1007_s11426_020_9955_y
crossref_primary_10_1002_smtd_202100762
crossref_primary_10_1021_acsnano_9b01622
crossref_primary_10_1016_j_snb_2020_129134
crossref_primary_10_1016_j_trac_2022_116745
crossref_primary_10_1016_j_snb_2021_130713
crossref_primary_10_1021_acs_analchem_1c01198
crossref_primary_10_1039_C8RA06973E
crossref_primary_10_1039_C8NR06699J
crossref_primary_10_1039_D2NR06020E
crossref_primary_10_1016_j_talanta_2020_121565
crossref_primary_10_1039_D0AN01948H
crossref_primary_10_20964_2019_03_57
crossref_primary_10_1021_acs_analchem_7b03474
crossref_primary_10_1039_D1AN00860A
crossref_primary_10_1007_s00604_019_3453_2
crossref_primary_10_1039_C8CS00303C
crossref_primary_10_1039_D0SC01534B
Cites_doi 10.1016/j.ab.2007.11.013
10.1021/la704078u
10.1021/acs.analchem.6b01063
10.1039/c3an00447c
10.1002/anie.200503345
10.1002/0471142700.nc1202s09
10.1021/ac900961y
10.1021/ac011167t
10.1016/j.mimet.2015.04.009
10.3791/52064
10.1021/acs.analchem.5b04648
10.1038/nbt1235
10.1146/annurev-bioeng-061008-124934
10.1038/nm.1755
10.1038/nprot.2006.51
10.1021/ac070772e
10.1016/j.bios.2010.07.090
10.1039/C4NR00625A
10.1093/nar/gnh101
10.1007/978-1-61779-319-6_18
10.1126/science.1088755
10.3855/jidc.3623
10.1038/nm1378
10.1021/ac2026783
10.1016/j.tibtech.2005.02.006
10.1039/c2an35054h
10.1002/smll.200500260
10.1016/j.bios.2016.10.065
10.1016/j.bios.2004.10.016
10.1021/ac0513764
10.1039/b606294f
10.1039/C2AN36374G
10.1038/srep38114
10.1016/j.jcis.2009.04.005
10.1021/nn900227d
10.1373/clinchem.2009.127266
10.1038/ncomms4367
10.1073/pnas.101124598
10.1039/c3lc41285g
10.1016/0361-9230(87)90129-8
10.1093/nar/gkl260
10.1039/c1cc12249e
10.1021/ac061592s
10.1016/S1369-7021(13)70014-2
10.1038/nprot.2007.201
10.1038/nbt1201
10.1039/c0cc00675k
10.1016/j.ab.2011.05.004
10.1016/j.aca.2014.04.055
10.1021/acs.analchem.5b02848
10.1038/nbt0502-473
10.1517/17530059.2011.607161
10.1021/ac101022m
10.1016/j.mam.2008.01.001
10.1002/adma.201301334
10.1021/am403816x
10.1002/asia.200900217
10.1021/ac2002559
10.1111/j.1538-7836.2007.02500.x
10.1007/s00018-008-8179-y
10.1073/pnas.170237197
10.1021/ac504206n
10.1016/j.bios.2013.01.047
10.1021/nl3004732
10.1039/C3NR05539F
10.1039/b712421j
ContentType Journal Article
Copyright Copyright © 2017 American Chemical Society
Copyright American Chemical Society Sep 5, 2017
Copyright_xml – notice: Copyright © 2017 American Chemical Society
– notice: Copyright American Chemical Society Sep 5, 2017
DBID NPM
AAYXX
CITATION
7QF
7QO
7QQ
7SC
7SE
7SP
7SR
7TA
7TB
7TM
7U5
7U7
7U9
8BQ
8FD
C1K
F28
FR3
H8D
H8G
H94
JG9
JQ2
KR7
L7M
L~C
L~D
P64
7X8
DOI 10.1021/acs.analchem.7b01535
DatabaseName PubMed
CrossRef
Aluminium Industry Abstracts
Biotechnology Research Abstracts
Ceramic Abstracts
Computer and Information Systems Abstracts
Corrosion Abstracts
Electronics & Communications Abstracts
Engineered Materials Abstracts
Materials Business File
Mechanical & Transportation Engineering Abstracts
Nucleic Acids Abstracts
Solid State and Superconductivity Abstracts
Toxicology Abstracts
Virology and AIDS Abstracts
METADEX
Technology Research Database
Environmental Sciences and Pollution Management
ANTE: Abstracts in New Technology & Engineering
Engineering Research Database
Aerospace Database
Copper Technical Reference Library
AIDS and Cancer Research Abstracts
Materials Research Database
ProQuest Computer Science Collection
Civil Engineering Abstracts
Advanced Technologies Database with Aerospace
Computer and Information Systems Abstracts – Academic
Computer and Information Systems Abstracts Professional
Biotechnology and BioEngineering Abstracts
MEDLINE - Academic
DatabaseTitle PubMed
CrossRef
Materials Research Database
Technology Research Database
Computer and Information Systems Abstracts – Academic
Mechanical & Transportation Engineering Abstracts
Nucleic Acids Abstracts
ProQuest Computer Science Collection
Computer and Information Systems Abstracts
Materials Business File
Environmental Sciences and Pollution Management
Aerospace Database
Copper Technical Reference Library
Engineered Materials Abstracts
Biotechnology Research Abstracts
AIDS and Cancer Research Abstracts
Advanced Technologies Database with Aerospace
ANTE: Abstracts in New Technology & Engineering
Civil Engineering Abstracts
Aluminium Industry Abstracts
Virology and AIDS Abstracts
Toxicology Abstracts
Electronics & Communications Abstracts
Ceramic Abstracts
METADEX
Biotechnology and BioEngineering Abstracts
Computer and Information Systems Abstracts Professional
Solid State and Superconductivity Abstracts
Engineering Research Database
Corrosion Abstracts
MEDLINE - Academic
DatabaseTitleList
MEDLINE - Academic
PubMed
Materials Research Database
Database_xml – sequence: 1
  dbid: NPM
  name: PubMed
  url: https://proxy.k.utb.cz/login?url=http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed
  sourceTypes: Index Database
DeliveryMethod fulltext_linktorsrc
Discipline Engineering
Chemistry
EISSN 1520-6882
EndPage 8973
ExternalDocumentID 10_1021_acs_analchem_7b01535
28780857
a040235561
Genre Research Support, Non-U.S. Gov't
Journal Article
GroupedDBID -
.K2
02
1AW
23M
53G
53T
55A
5GY
5RE
5VS
7~N
85S
AABXI
ABFLS
ABMVS
ABOCM
ABPPZ
ABPTK
ABUCX
ABUFD
ACGFS
ACGOD
ACIWK
ACJ
ACNCT
ACPRK
ACS
AEESW
AENEX
AFEFF
AFRAH
ALMA_UNASSIGNED_HOLDINGS
AQSVZ
BAANH
BKOMP
CS3
D0L
DZ
EBS
ED
ED~
EJD
F20
F5P
GNL
IH9
IHE
JG
JG~
K2
P2P
PQEST
PQQKQ
ROL
RXW
TAE
TN5
UHB
UI2
UKR
VF5
VG9
VQA
W1F
WH7
X
X6Y
XFK
YZZ
---
-DZ
-~X
.DC
4.4
6J9
AAHBH
ABHFT
ABHMW
ABJNI
ABQRX
ACBEA
ACGFO
ACKOT
ADHLV
AGXLV
AHGAQ
CUPRZ
GGK
KZ1
LMP
NPM
XSW
ZCA
~02
AAYXX
CITATION
7QF
7QO
7QQ
7SC
7SE
7SP
7SR
7TA
7TB
7TM
7U5
7U7
7U9
8BQ
8FD
C1K
F28
FR3
H8D
H8G
H94
JG9
JQ2
KR7
L7M
L~C
L~D
P64
7X8
ID FETCH-LOGICAL-a376t-d180b669ffd1fa178b118050ec1c4ead2218200c8631071b361c6e65e4e454b73
IEDL.DBID ACS
ISSN 0003-2700
IngestDate Fri Aug 16 23:09:41 EDT 2024
Thu Oct 10 17:10:25 EDT 2024
Fri Aug 23 03:01:03 EDT 2024
Wed Oct 16 00:51:50 EDT 2024
Thu Aug 27 13:43:03 EDT 2020
IsPeerReviewed true
IsScholarly true
Issue 17
Language English
LinkModel DirectLink
MergedId FETCHMERGED-LOGICAL-a376t-d180b669ffd1fa178b118050ec1c4ead2218200c8631071b361c6e65e4e454b73
Notes ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
ORCID 0000-0001-9978-3890
PMID 28780857
PQID 1942213932
PQPubID 45400
PageCount 8
ParticipantIDs proquest_miscellaneous_1926979726
proquest_journals_1942213932
crossref_primary_10_1021_acs_analchem_7b01535
pubmed_primary_28780857
acs_journals_10_1021_acs_analchem_7b01535
ProviderPackageCode JG~
55A
AABXI
GNL
VF5
7~N
ACJ
VG9
W1F
ACS
AEESW
AFEFF
.K2
ABMVS
ABUCX
IH9
BAANH
AQSVZ
ED~
UI2
PublicationCentury 2000
PublicationDate 20170905
2017-09-05
PublicationDateYYYYMMDD 2017-09-05
PublicationDate_xml – month: 09
  year: 2017
  text: 20170905
  day: 05
PublicationDecade 2010
PublicationPlace United States
PublicationPlace_xml – name: United States
– name: Washington
PublicationTitle Analytical chemistry (Washington)
PublicationTitleAlternate Anal. Chem
PublicationYear 2017
Publisher American Chemical Society
Publisher_xml – name: American Chemical Society
References ref9/cit9
ref45/cit45
ref3/cit3
ref27/cit27
ref63/cit63
ref56/cit56
ref16/cit16
ref52/cit52
ref23/cit23
ref8/cit8
ref31/cit31
ref59/cit59
ref2/cit2
ref34/cit34
ref37/cit37
ref20/cit20
ref48/cit48
ref60/cit60
ref17/cit17
ref10/cit10
ref35/cit35
ref53/cit53
ref19/cit19
ref21/cit21
ref42/cit42
ref46/cit46
ref49/cit49
ref13/cit13
ref61/cit61
ref24/cit24
ref38/cit38
ref50/cit50
ref64/cit64
ref54/cit54
ref6/cit6
ref36/cit36
ref18/cit18
ref65/cit65
ref11/cit11
ref25/cit25
ref29/cit29
ref32/cit32
ref39/cit39
ref14/cit14
ref57/cit57
ref5/cit5
ref51/cit51
ref43/cit43
ref28/cit28
ref40/cit40
ref26/cit26
ref55/cit55
ref12/cit12
ref15/cit15
ref62/cit62
ref66/cit66
ref41/cit41
ref58/cit58
ref22/cit22
ref33/cit33
ref4/cit4
ref30/cit30
ref47/cit47
ref1/cit1
ref44/cit44
ref7/cit7
References_xml – ident: ref25/cit25
  doi: 10.1016/j.ab.2007.11.013
– ident: ref33/cit33
  doi: 10.1021/la704078u
– ident: ref48/cit48
  doi: 10.1021/acs.analchem.6b01063
– ident: ref13/cit13
  doi: 10.1039/c3an00447c
– ident: ref19/cit19
  doi: 10.1002/anie.200503345
– ident: ref57/cit57
  doi: 10.1002/0471142700.nc1202s09
– ident: ref24/cit24
  doi: 10.1021/ac900961y
– ident: ref54/cit54
  doi: 10.1021/ac011167t
– ident: ref43/cit43
  doi: 10.1016/j.mimet.2015.04.009
– ident: ref45/cit45
  doi: 10.3791/52064
– ident: ref14/cit14
  doi: 10.1021/acs.analchem.5b04648
– ident: ref1/cit1
  doi: 10.1038/nbt1235
– ident: ref46/cit46
  doi: 10.1146/annurev-bioeng-061008-124934
– ident: ref7/cit7
  doi: 10.1038/nm.1755
– ident: ref55/cit55
  doi: 10.1038/nprot.2006.51
– ident: ref42/cit42
  doi: 10.1021/ac070772e
– ident: ref34/cit34
  doi: 10.1016/j.bios.2010.07.090
– ident: ref10/cit10
  doi: 10.1039/C4NR00625A
– ident: ref64/cit64
  doi: 10.1093/nar/gnh101
– ident: ref32/cit32
  doi: 10.1007/978-1-61779-319-6_18
– ident: ref41/cit41
  doi: 10.1126/science.1088755
– ident: ref47/cit47
  doi: 10.3855/jidc.3623
– ident: ref6/cit6
  doi: 10.1038/nm1378
– ident: ref20/cit20
  doi: 10.1021/ac2026783
– ident: ref16/cit16
  doi: 10.1016/j.tibtech.2005.02.006
– ident: ref28/cit28
  doi: 10.1039/c2an35054h
– ident: ref39/cit39
  doi: 10.1002/smll.200500260
– ident: ref44/cit44
  doi: 10.1016/j.bios.2016.10.065
– ident: ref9/cit9
  doi: 10.1016/j.bios.2004.10.016
– ident: ref36/cit36
  doi: 10.1021/ac0513764
– ident: ref35/cit35
  doi: 10.1039/b606294f
– ident: ref11/cit11
  doi: 10.1039/C2AN36374G
– ident: ref37/cit37
  doi: 10.1038/srep38114
– ident: ref50/cit50
  doi: 10.1016/j.jcis.2009.04.005
– ident: ref65/cit65
  doi: 10.1021/nn900227d
– ident: ref18/cit18
  doi: 10.1373/clinchem.2009.127266
– ident: ref62/cit62
  doi: 10.1038/ncomms4367
– ident: ref23/cit23
  doi: 10.1073/pnas.101124598
– ident: ref27/cit27
  doi: 10.1039/c3lc41285g
– ident: ref4/cit4
  doi: 10.1016/0361-9230(87)90129-8
– ident: ref17/cit17
  doi: 10.1093/nar/gkl260
– ident: ref26/cit26
  doi: 10.1039/c1cc12249e
– ident: ref12/cit12
  doi: 10.1021/ac061592s
– ident: ref66/cit66
  doi: 10.1016/S1369-7021(13)70014-2
– ident: ref38/cit38
  doi: 10.1038/nprot.2007.201
– ident: ref15/cit15
  doi: 10.1038/nbt1201
– ident: ref3/cit3
  doi: 10.1039/c0cc00675k
– ident: ref22/cit22
  doi: 10.1016/j.ab.2011.05.004
– ident: ref59/cit59
  doi: 10.1016/j.aca.2014.04.055
– ident: ref8/cit8
  doi: 10.1021/acs.analchem.5b02848
– ident: ref21/cit21
  doi: 10.1038/nbt0502-473
– ident: ref31/cit31
  doi: 10.1517/17530059.2011.607161
– ident: ref61/cit61
  doi: 10.1021/ac101022m
– ident: ref53/cit53
  doi: 10.1016/j.mam.2008.01.001
– ident: ref2/cit2
  doi: 10.1002/adma.201301334
– ident: ref63/cit63
  doi: 10.1021/am403816x
– ident: ref40/cit40
  doi: 10.1002/asia.200900217
– ident: ref60/cit60
  doi: 10.1021/ac2002559
– ident: ref51/cit51
  doi: 10.1111/j.1538-7836.2007.02500.x
– ident: ref52/cit52
  doi: 10.1007/s00018-008-8179-y
– ident: ref5/cit5
  doi: 10.1073/pnas.170237197
– ident: ref58/cit58
  doi: 10.1021/ac504206n
– ident: ref30/cit30
  doi: 10.1016/j.bios.2013.01.047
– ident: ref49/cit49
  doi: 10.1021/nl3004732
– ident: ref29/cit29
  doi: 10.1039/C3NR05539F
– ident: ref56/cit56
  doi: 10.1039/b712421j
SSID ssj0011016
Score 2.4234624
Snippet We have devised a barcode DNA-mediated signal amplifying strategy for ultrasensitive biomolecular detection by utilizing matrix-assisted laser desorption...
SourceID proquest
crossref
pubmed
acs
SourceType Aggregation Database
Index Database
Publisher
StartPage 8966
SubjectTerms Amplification
Aptamers
Beads
Biomolecules
Chemistry
Cyanides
Deoxyribonucleic acid
Desorption
DNA
Gold
Ionization
Magnetic separation
Mass spectrometry
Mass spectroscopy
Molecules
Nanoparticles
Potassium
Potassium cyanide
Spectroscopy
Strategy
Thrombin
Title Barcode DNA-Mediated Signal Amplifying Strategy for Ultrasensitive Biomolecular Detection on Matrix-Assisted Laser Desorption Ionization Time of Flight (MALDI-TOF) Mass Spectrometry
URI http://dx.doi.org/10.1021/acs.analchem.7b01535
https://www.ncbi.nlm.nih.gov/pubmed/28780857
https://www.proquest.com/docview/1942213932
https://search.proquest.com/docview/1926979726
Volume 89
hasFullText 1
inHoldings 1
isFullTextHit
isPrint
link http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwlV1Lb9QwELagHIADj_JaKMhIHOjB28Rx7OS43WXVom572K7UW-TYDqroJlWTlYD_xf9jxhsvBVQBUi5JHDuOx55vMp75CHmXGGUc4CDmMm6ZcGCnZNZoxi2g18gKbjwP2exYHizEx7P07Keh-LsHn8d72rRDDR8V-rAcqhLUV5LeJnc4qEY0tkbj-cZrgJZoYMhDh2oIlbuhFlRIpv1VId2AMr22mT4kJyFmZ73J5PNw1ZVD8-3PFI7_2JFH5EEPPOloLSmPyS1Xb5O748D3tk3uX0tN-IR834cZ0FhHJ8cjNvN8Hs7S-fknXwluQ_cBUrTPbvuVAviliws4a3FLPC6idP-8WQb2XTpxnd_1VVM4ZkgM8IWBbKCUWXoET2GRtrnySxg9bEKAKMUgFdpUdHqB_xHo-9noaHLITk-mu1BN29L5pSfyWTroxVOymH44HR-wnuKBaVjZOmbjLCqlzKvKxpWOVVZiSro0ciY2AoSc-wTzkckkwFAVl4mMjXQydcKJVJQqeUa26qZ2LwjNY22sjpwTuhTIllgpK6w1NrXaaqUHZBdGoOinaFt47zuPC7wYhqXoh2VAWJCJ4nKd9eMv5XeC4FxrIBfw-gkA5AF5u7kNg4ouGV27ZoVluMxVrrgckOdrgds0CLZshvwDL__jxV-RexxxB3q80h2y1V2t3GtATV35xk-VH-oAFbQ
link.rule.ids 315,786,790,2782,27107,27955,27956,57091,57141
linkProvider American Chemical Society
linkToHtml http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwlV1Lj9MwEB4ty2HhwGN5FRYwEgf2kJKHEyfHbkvVQlMObdHeIsd20IptstqkEvC_-H_MuElZkFZopVziOH7EY8_njGc-gLeBEsogDnJM7GuHG9ynxFpJx9eIXl3NfWV5yNJ5NFnxj6fh6R6EnS8MNqLGkmprxP8TXcB7T2kSvy12Zd0XOWqxILwFt0OBCo8Q0XCxMx7QhrQjyiO7aucxd00ppJdU_bdeugZsWqUzvg9fds21Z02-9TdN3lc__4nkeOP-PIB7LQxlg63cPIQ9Ux7CwbBjfzuEu1cCFT6CXyc4Hypt2Gg-cFLL7mE0W5x9tYXQoXTrLsXaWLc_GEJhtjrHu5oOyNOSyk7OqnXHxctGprFnwEqGV0o0Ad8dlBSSOc1m-BZlqatLu6CxadW5izJyWWFVwcbn9FeBvUsHs9HUWX4eH2Mxdc0WF5bWZ22wF49hNf6wHE6clvDBkbjONY72YjePoqQotFdIT8Q5BagLXaM8xVHkfRtu3lVxhKBUeHkQeSoyUWi44SHPRfAE9suqNM-AJZ5UWrrGcJlz4k4shOZaKx1qqaWQPTjGEcjaCVtn1hbvexkldsOStcPSA6cTjexiGwPkP_mPOvm5UkHCsfkBwuUevNk9xkElA40sTbWhPH6UiET4UQ-ebuVuVyHubGNiI3h-g4a_hoPJMp1ls-n80wu44xMiIVtYeAT7zeXGvEQ81eSv7Oz5DTPUHh8
linkToPdf http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwnV1Lj9MwELZgkXgceCyvwgJG4sAeXOLEiZNjtyXaQluQupVWe4kc20Ertkm1SSXgf_H_mHGTqiCtEEi5xHH8iGfszxnPfIS8CbTUFnAQs7FvmLCwT4mNVsw3gF49I3zteMims-h4IT6chqc7VF_QiBpKqp0RH7V6ZYo2wgB_h-kKvi90Z9mXOaxkQXid3AglF6iTg-F8a0DATWlHloe21c5r7opScG3S9e9r0xWA0y086T1ytm2yO2_ytb9u8r7-8Uc0x__q031yt4WjdLCRnwfkmi33ya1hxwK3T-7sBCx8SH4egV5UxtLRbMCmjuXDGjo__-IKwcPpzm2KtjFvv1OAxHRxAXc1HpTHqZUenVfLjpOXjmzjzoKVFK4p0gV8YyAxKHuGTuAtzFJXl25io-Oqcxul6LpCq4KmF_h3gb6dDiajMTv5lB5CMXVN5ytH77O00ItHZJG-Pxkes5b4gSmY7xpmeOzlUZQUheGF4jLOMVBd6FnNtQDR913YeU_HEYBTyfMg4jqyUWiFFaHIZfCY7JVVaZ8SmnCljfKsFSoXyKFYSCOM0SY0yiipeuQQRiBrFbfOnE3e5xkmdsOStcPSI6wTj2y1iQXyl_wHnQztVJAIaH4AsLlHXm8fw6CioUaVtlpjHj9KZCL9qEeebGRvWyHscGNkJXj2Dw1_RW5-HqXZZDz7-Jzc9hGYoEksPCB7zeXavgBY1eQvnQL9Aiu4IJk
openUrl ctx_ver=Z39.88-2004&ctx_enc=info%3Aofi%2Fenc%3AUTF-8&rfr_id=info%3Asid%2Fsummon.serialssolutions.com&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Ajournal&rft.genre=article&rft.atitle=Barcode+DNA-Mediated+Signal+Amplifying+Strategy+for+Ultrasensitive+Biomolecular+Detection+on+Matrix-Assisted+Laser+Desorption+Ionization+Time+of+Flight+%28MALDI-TOF%29+Mass+Spectrometry&rft.jtitle=Analytical+chemistry+%28Washington%29&rft.au=Ahmad%2C+Raheel&rft.au=Jang%2C+Hyowon&rft.au=Batule%2C+Bhagwan+S&rft.au=Park%2C+Hyun+Gyu&rft.date=2017-09-05&rft.pub=American+Chemical+Society&rft.issn=0003-2700&rft.eissn=1520-6882&rft.volume=89&rft.issue=17&rft.spage=8966&rft.epage=8973&rft_id=info:doi/10.1021%2Facs.analchem.7b01535&rft.externalDocID=a040235561
thumbnail_l http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/lc.gif&issn=0003-2700&client=summon
thumbnail_m http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/mc.gif&issn=0003-2700&client=summon
thumbnail_s http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/sc.gif&issn=0003-2700&client=summon