In Vitro Release of Lysozyme from Gelatin Microspheres: Effect of Cross-linking Agents and Thermoreversible Gel as Suspending Medium

• Published in: Biomacromolecules Vol. 12; no. 9; pp. 3186 - 3193
• Main Authors: Hiwale, Pradip, Lampis, Sandrina, Conti, Gabriele, Caddeo, Carla, Murgia, Sergio, Fadda, Anna M, Monduzzi, Maura
• Format: Journal Article
• Language: English
• Published: Washington, DC American Chemical Society 12.09.2011
• Subjects: Animals; Applied sciences; Biological and medical sciences; Chickens; Cross-Linking Reagents - chemistry; Delayed-Action Preparations - chemical synthesis; Delayed-Action Preparations - metabolism; Drug Carriers - chemical synthesis; Drug Carriers - metabolism; Exact sciences and technology; Excipients - chemistry; Gelatin - chemistry; Gels - chemistry; General pharmacology; Glucose - chemistry; Glutaral - chemistry; Medical sciences; Microscopy, Electron, Scanning; Microspheres; Muramidase - analysis; Muramidase - metabolism; Natural polymers; Particle Size; Pharmaceutical technology. Pharmaceutical industry; Pharmacology. Drug treatments; Phase Transition; Physicochemistry of polymers; Poloxamer - chemistry; Proteins; Spectroscopy, Fourier Transform Infrared; Temperature; Surfactant polymer; Drug carrier; Crosslink agent; Glucose; Glutaral; Gelatin; Crosslinked polymer; Microparticle; Propylene oxide copolymer; Release; Lysozyme; Enzyme; Control release polymer; Non ionic surfactant; Physical gel; Crosslinking; Particle suspension; Ethylene oxide copolymer; Experimental study; In vitro; Protein; Glycosylases; Triblock copolymer; Particle size distribution; Gelatin derivatives; Gel point; Glycosidases; Morphology; Hydrolases; Kinetics; Spherical particle; Lattice structure; Comparative study

This study was aimed to characterize the microstructure and the performance of gelatin microspheres (GMs) cross-linked by two different cross-linkers viz. d-glucose and glutaraldehyde. New formulations were obtained, suspending the GMs in a thermoreversible Pluronic F127 (PF127) liquid-crystalline g...