CHRISTMAS: Chiral Pair Isobaric Labeling Strategy for Multiplexed Absolute Quantitation of Enantiomeric Amino Acids

• Published in: Analytical chemistry (Washington) Vol. 95; no. 50; pp. 18504 - 18513
• Main Authors: Zhu, Zhijun, Xu, Shuling, Wang, Zicong, Delafield, Daniel G., Rigby, Michael J., Lu, Gaoyuan, Gu, Ting-Jia, Liu, Peng-Kai, Ma, Min, Puglielli, Luigi, Li, Lingjun
• Format: Journal Article
• Language: English
• Published: United States American Chemical Society 19.12.2023
• Subjects: Alzheimer's disease; Amines; Amino acids; Amino Acids - analysis; Animals; Chromatography, Liquid - methods; Enantiomers; Ionic mobility; Isobars; Isomers; Labeling; Leucine; Leucine - chemistry; Mass spectrometry; Mass spectroscopy; Mice; Multiplexing; Neurodegenerative diseases; Proteomics - methods; Quantitation; Reagents; Separation

Amino acids (AAs) in the d-form are involved in multiple pivotal neurological processes, although their l-enantiomers are most commonly found. Mass spectrometry-based analysis of low-abundance d-AAs has been hindered by challenging enantiomeric separation from l-AAs, low sensitivity for detection, a...