Multiplexed SERS Detection of Soluble Cancer Protein Biomarkers with Gold–Silver Alloy Nanoboxes and Nanoyeast Single-Chain Variable Fragments

• Published in: Analytical chemistry (Washington) Vol. 90; no. 17; pp. 10377 - 10384
• Main Authors: Li, Junrong, Wang, Jing, Grewal, Yadveer S, Howard, Christopher B, Raftery, Lyndon J, Mahler, Stephen, Wang, Yuling, Trau, Matt
• Format: Journal Article
• Language: English
• Published: United States American Chemical Society 04.09.2018
• Subjects: Alloys - chemistry; Biomarkers; Biomarkers, Tumor - metabolism; Cancer; Cancer screening; Chains; Chemistry; Early Detection of Cancer; Enzyme-Linked Immunosorbent Assay; Fragments; Gold; Gold - chemistry; Gold alloys; Gold base alloys; Growth factors; Humans; Limit of Detection; Medical treatment; Metal Nanoparticles - chemistry; Microscopy, Electron, Scanning; Microscopy, Electron, Transmission; Monoclonal antibodies; Multiplexing; Nanostructured materials; Neoplasm Proteins - metabolism; Neoplasms - diagnosis; Patients; PD-1 protein; Proteins; Raman spectra; Sensitivity; Silver; Silver - chemistry; Silver base alloys; Single-Chain Antibodies - chemistry; Spectrum Analysis, Raman - methods

Highly sensitive, multiplexed detection of soluble cancer protein biomarkers can facilitate early cancer screening as well as enable real-time monitoring of patients’ sensitivity and resistance to therapy. Current technologies for detection of soluble cancer protein biomarkers, e.g., enzyme-linked i...