Light-Independent Metabolomics of Endophytic Thielavia subthermophila Provides Insight into Microbial Hypericin Biosynthesis

• Published in: Journal of natural products (Washington, D.C.) Vol. 72; no. 10; pp. 1825 - 1835
• Main Authors: Kusari, Souvik, Zühlke, Sebastian, Košuth, Ján, Čellárová, Eva, Spiteller, Michael
• Format: Journal Article
• Language: English
• Published: Northbrook, IL American Chemical Society and American Society of Pharmacognosy 23.10.2009; American Society of Pharmacognosy
• Subjects: Antibiotics, microbial producers, chemotherapic agents, antiseptics, disinfecting agents; Antineoplastic Agents - chemistry; Antineoplastic Agents - isolation & purification; Antineoplastic Agents - pharmacology; Applied microbiology; Base Sequence; Biological and medical sciences; Chemotherapic agents; Drug Screening Assays, Antitumor; Emodin - metabolism; Fundamental and applied biological sciences. Psychology; Humans; Hypericum - microbiology; Hypericum perforatum; Light; Metabolomics; Microbiology; Molecular Structure; Perylene - analogs & derivatives; Perylene - metabolism; Sordariales - chemistry; Sordariales - genetics; Antineoplastic agent; Hypericum perforatum; Metabolomics; Axenic culture; Metabolite; Guttiferae; Leukemia; Cytotoxicity; Biosynthesis; Extract; Medicinal plant; Fungi; Dicotyledones; Angiospermae; Quinonemethide; Anthraquinone(6-methyl 1,3,8-trihydroxy); Tumor cell; Ascomycota; Human; Quinone; In vitro; Biological activity; Cell line; Submerged culture; Endophyte; Spermatophyta; Kinetics; Photosensitizer

The possible microbial mechanism of hypericin (1) and emodin (2) biosynthesis was studied in axenic submerged culture conditions in the endophytic fungus Thielavia subthermophila, isolated from Hypericum perforatum. The growth and secondary metabolite production of the endophyte remained independent...