O‑Glycopeptide Truncation Strategy for Heterogeneous O‑GalNAc Glycoproteomics Characterization

• Published in: Analytical chemistry (Washington) Vol. 95; no. 26; pp. 10017 - 10024
• Main Authors: Liu, Luyao, Zhu, He, Liu, Lei, You, Xin, Mao, Jiawei, Wang, Yan, Liu, Xiaoyan, Qin, Hongqiang, Dong, Mingming, Ye, Mingliang
• Format: Journal Article
• Language: English
• Published: United States American Chemical Society 04.07.2023
• Subjects: Biological activity; Biological properties; Biological samples; Chemistry; Enrichment; Glycan; Glycopeptides; Glycopeptides - analysis; Glycoproteins; Glycoproteins - chemistry; Glycosylation; Humans; Mass spectrometry; Mass spectroscopy; Peptides; Polysaccharides; Polysaccharides - analysis; Proteins; Proteolysis; Proteomics - methods; Sialic acids

Mucin-type O-glycosylation (or O-GalNAcylation) takes place on most membrane and secretory proteins and is vital in regulating protein functions and many biological processes. O-GalNAcylation generally exhibits highly diverse and dense O-glycans linked to carrier proteins, which challenges the analy...