High-Throughput Single-Cell Immunoassay in the Cellular Native Environment Using Online Desalting Dual-Spray Mass Spectrometry

• Published in: Analytical chemistry (Washington) Vol. 92; no. 24; pp. 15854 - 15861
• Main Authors: Xu, Shuting, Xue, Jinjuan, Bai, Yu, Liu, Huwei
• Format: Journal Article
• Language: English
• Published: United States American Chemical Society 15.12.2020
• Subjects: Antigens; Biomarkers, Tumor - analysis; Buffers; CA-125 Antigen - analysis; Cancer; Cell surface; Chemistry; Desalination; Environments; High-Throughput Screening Assays; Humans; Immunoassay; Ionization; Ions; Lipids; Mass Spectrometry; Mass spectroscopy; Particle Size; Proteins; Rhodamine; Salts; Scientific imaging; Single-Cell Analysis; Spectroscopy; Surface Properties; Tags; Tumor cell lines; Tumor Cells, Cultured

Single-cell mass spectrometry (MS) remains challenging in the analysis of cells in the native environment due to the severe ion suspension from nonvolatile salts. Synchronous desalting and ionization would be ideal to both ensure the native environment and remove the salt interference. Here, a novel...