Preparation of Poly(ethylene glycol) Protected Nanoparticles with Variable Bioconjugate Ligand Density

• Published in: Biomacromolecules Vol. 9; no. 10; pp. 2705 - 2711
• Main Authors: Gindy, Marian E, Ji, Shengxiang, Hoye, Thomas R, Panagiotopoulos, Athanassios Z, Prud’homme, Robert K
• Format: Journal Article
• Language: English
• Published: Washington, DC American Chemical Society 01.10.2008
• Subjects: Animals; Applied sciences; Biological and medical sciences; Cattle; Exact sciences and technology; Forms of application and semi-finished materials; General pharmacology; Ligands; Light; Maleimides - chemistry; Medical sciences; Miscellaneous; Molecular Conformation; Nanoparticles - chemistry; Nanotechnology - methods; Particle Size; Pharmaceutical technology. Pharmaceutical industry; Pharmacology. Drug treatments; Polyethylene Glycols - chemistry; Polymer industry, paints, wood; Polymers - chemistry; Scattering, Radiation; Serum Albumin, Bovine - chemistry; Sulfhydryl Compounds - chemistry; Surface Properties; Technology of polymers; Surface reaction; End group; Chemical precipitation; Nanoparticle; Control release polymer; Drug carrier; Serum albumin; Ethylene oxide copolymer; Experimental study; Protein; Chemical coupling; Particle size distribution; Chemical modification; Caprolactone copolymer; Diblock copolymer; Manufacturing; Chemical reactivity; Aliphatic copolymer; Amphiphilic polymer

Maleimide-functional poly(ethylene glycol)-b-poly(ε-caprolactone) nanoparticles (NPs) were prepared via the Flash NanoPrecipitation technique. Subsequent reaction with a model ligand, bovine serum albumin (BSA), was conducted using thiol-maleimide conjugation. Reaction of up to 22% of NP surface mal...