Enzymatic on-Chip Enhancement for High Resolution Genotyping DNA Microarrays

• Published in: Analytical chemistry (Washington) Vol. 84; no. 11; pp. 5080 - 5084
• Main Authors: Schulze, Holger, Barl, Timo, Vase, Hollie, Baier, Shiromi, Thomas, Peter, Giraud, Gerard, Crain, Jason, Bachmann, Till T
• Format: Journal Article
• Language: English
• Published: Washington, DC American Chemical Society 05.06.2012
• Subjects: Analytical chemistry; Bacterial Proteins - chemistry; Bacterial Proteins - genetics; Bacterial Typing Techniques - methods; Base Pair Mismatch; beta-Lactam Resistance - genetics; beta-Lactamases - chemistry; beta-Lactamases - genetics; Chemistry; Drug resistance; Endonucleases - metabolism; Enzymes; Exact sciences and technology; Fluorescence; General, instrumentation; Genotype; Genotype & phenotype; Hybridization; Klebsiella pneumoniae - genetics; Klebsiella pneumoniae - isolation & purification; Nucleic Acid Hybridization; Oligonucleotide Array Sequence Analysis - methods; Oligonucleotide Probes - chemistry; Polymorphism, Single Nucleotide; Spectrometry, Fluorescence; Thermodynamics; Fidelity; High resolution; Microanalysis; DNA chip; Esterases; Identification; Probe; Pathogenic; Bacteria; Oligonucleotide; Nuclease; Enterobacteriaceae; Human; Fluorescence detector; False positive; Enzyme; System on a chip; Chemical sensor; Real time; Resistance; Variant; Enzymatic method; Total internal reflection; Allele; Antibiotic; Thermodynamic parameter; Nucleotide; Hydrolases; End; Kinetics; Single nucleotide polymorphism; Sensor array; Application; Klebsiella pneumoniae

Antibiotic resistance among pathogenic microorganisms is emerging as a major human healthcare concern. While there are a variety of resistance mechanisms, many can be related to single nucleotide polymorphisms and for which DNA microarrays have been widely deployed in bacterial genotyping. However,...