Development of a Secondary Antibody Thio-Functionalized Microcantilever Immunosensor and an ELISA for Measuring Ginsenoside Re Content in the Herb Ginseng

• Published in: Analytical chemistry (Washington) Vol. 84; no. 10; pp. 4327 - 4333
• Main Authors: Nan, Tiegui, Wu, Shangquan, Zhao, Hongwei, Tan, Weiming, Li, Zhaohu, Zhang, Qingchuan, Wang, Baomin
• Format: Journal Article
• Language: English
• Published: Washington, DC American Chemical Society 15.05.2012
• Subjects: Analytical chemistry; Animals; Antibodies, Monoclonal - chemistry; Antibodies, Monoclonal - immunology; Biosensors; Cattle; Chemistry; Chromatographic methods and physical methods associated with chromatography; Enzyme-Linked Immunosorbent Assay; Exact sciences and technology; Female; General, instrumentation; Ginsenosides - analysis; Ginsenosides - immunology; Goats; Haptens - chemistry; Haptens - immunology; Herbs; Immunoassay; Immunoglobulin G - immunology; Mice; Mice, Inbred BALB C; Miscellaneous; Monoclonal antibodies; Other chromatographic methods; Panax - chemistry; Serum Albumin, Bovine - chemistry; Serum Albumin, Bovine - immunology; Sulfhydryl Compounds - chemistry; Immunoglobulins; Chemical analysis; Bovine; Microsensor; Enzyme; Sample; HPLC chromatography; IgG; Immobilization; Serum albumin; Monoclonal antibody; Concentration; Chemical sensor; Enzyme immunoassay; Immunosensor; Vertebrata; Sensitivity; Mammalia; Biosensor; Artiodactyla; Inhibition; ELISA assay; Ungulata

Ginsenoside Re (GRe) is a major active component of the Chinese medicinal herb ginseng, Panax ginseng. A sensitive and specific monoclonal antibody (mAb), designated as mAb3D6, was generated with a GRe–bovine serum albumin conjugate as an immunogen. Microcantilever immunosensors (MCS), one modified...