Improved Protein Hydrogen/Deuterium Exchange Mass Spectrometry Platform with Fully Automated Data Processing

• Published in: Analytical chemistry (Washington) Vol. 84; no. 11; pp. 4942 - 4949
• Main Authors: Zhang, Zhongqi, Zhang, Aming, Xiao, Gang
• Format: Journal Article
• Language: English
• Published: Washington, DC American Chemical Society 05.06.2012
• Subjects: Analytical chemistry; Antibodies, Monoclonal - chemistry; Automation, Laboratory; Chemical compounds; Chemistry; Data processing; Deuterium - chemistry; Deuterium Exchange Measurement; Exact sciences and technology; Guanidine - chemistry; Hydrogen - chemistry; Immunoglobulin G - chemistry; Mass Spectrometry; Models, Chemical; Peptide Hydrolases - chemistry; Peptides - analysis; Proteases; Protein Conformation; Proteins; Reference Standards; Software; Spectrometric and optical methods; Deuterium; Peptides; Hydrogen; Bias; Internal standard; Identification; Monoclonal antibody; Mixture; Design; Dynamics; Protection; Measurement error; Data analysis; Enzyme; Intrinsic protein; Random error; Data processing; Guanidines; Concentration; Digestion; Method; Automatic processing; Peptidases; Transition; Hydrolases; Software; Detection; Mass spectrometry; Conformation

Protein hydrogen/deuterium exchange (HDX) followed by protease digestion and mass spectrometric (MS) analysis is accepted as a standard method for studying protein conformation and conformational dynamics. In this article, an improved HDX MS platform with fully automated data processing is described...