Cleavable Biotin Probes for Labeling of Biomolecules via Azide−Alkyne Cycloaddition

• Published in: Journal of the American Chemical Society Vol. 132; no. 51; pp. 18351 - 18360
• Main Authors: Szychowski, Janek, Mahdavi, Alborz, Hodas, Jennifer J. L, Bagert, John D, Ngo, John T, Landgraf, Peter, Dieterich, Daniela C, Schuman, Erin M, Tirrell, David A
• Format: Journal Article
• Language: English
• Published: WASHINGTON American Chemical Society 29.12.2010; Amer Chemical Soc
• Subjects: Alkynes - chemistry; Azides - chemistry; Biotin - chemistry; Chemistry; Chemistry, Multidisciplinary; Cyclization; Dapsone; Green Fluorescent Proteins - chemistry; Molecular Probes - chemical synthesis; Molecular Probes - chemistry; Physical Sciences; Protein Structure, Secondary; Proteins - chemistry; Science & Technology; REPORTER; ANALOGS; RECOMBINANT PROTEINS; IN-VIVO; SOLID-PHASE; CHEMISTRY; LIGATION; TERMINAL ALKYNES; IDENTIFICATION; PROTEOMES

The azide−alkyne cycloaddition provides a powerful tool for bio-orthogonal labeling of proteins, nucleic acids, glycans, and lipids. In some labeling experiments, e.g., in proteomic studies involving affinity purification and mass spectrometry, it is convenient to use cleavable probes that allow rel...