Profiling Human Serum Antibodies with a Carbohydrate Antigen Microarray

• Published in: Journal of proteome research Vol. 8; no. 9; pp. 4301 - 4310
• Main Authors: Oyelaran, Oyindasola, McShane, Lisa M, Dodd, Lori, Gildersleeve, Jeffrey C
• Format: Journal Article
• Language: English
• Published: United States American Chemical Society 01.09.2009
• Subjects: Adult; Analysis of Variance; Antibodies - blood; Antibodies - immunology; Antigens - chemistry; Antigens - immunology; Blood Group Antigens; Cluster Analysis; Humans; Longitudinal Studies; Male; Microarray Analysis - methods; Polysaccharides - chemistry; Polysaccharides - immunology; Proteomics - methods; Regression Analysis; Reproducibility of Results; Glycan array; microarray; variability; serum antibodies; normalization; carbohydrate antigens

Carbohydrate antigen arrays (glycan arrays) have been recently developed for the high-throughput analysis of carbohydrate macromolecule interactions. When profiling serum, information about experimental variability, interindividual biological variability, and intraindividual temporal variability is...