Conformational Dynamics of α‑Synuclein during the Interaction with Phospholipid Nanodiscs by Millisecond Hydrogen–Deuterium Exchange Mass Spectrometry

• Published in: Journal of the American Society for Mass Spectrometry Vol. 32; no. 5; pp. 1169 - 1179
• Main Authors: Oganesyan, Irina, Lento, Cristina, Tandon, Anurag, Wilson, Derek J
• Format: Journal Article
• Language: English
• Published: United States American Chemical Society 05.05.2021
• Subjects: phospholipids nanodiscs; mass spectrometry; conformational dynamics; α-synuclein; Parkinson’s disease; hydrogen−deuterium exchange

Both normal and pathological functions of α-synuclein (αSN), an abundant protein in the central and peripheral nervous system, have been linked to its interaction with membrane lipid bilayers. The ability to characterize structural transitions of αSN upon membrane complexation will clarify molecular...