Nanofiber Dressings Topically Delivering Molecularly Engineered Human Cathelicidin Peptides for the Treatment of Biofilms in Chronic Wounds
Biofilms of multidrug-resistant bacteria in chronic wounds pose a great challenge in wound care. Herein, we report the topical delivery of molecularly engineered antimicrobial peptides using electrospun nanofiber dressings as a carrier for the treatment of biofilms of multidrug-resistant bacteria in...
Saved in:
Published in | Molecular pharmaceutics Vol. 16; no. 5; pp. 2011 - 2020 |
---|---|
Main Authors | , , , , , , , , , |
Format | Journal Article |
Language | English |
Published |
United States
American Chemical Society
06.05.2019
|
Subjects | |
Online Access | Get full text |
Cover
Loading…
Abstract | Biofilms of multidrug-resistant bacteria in chronic wounds pose a great challenge in wound care. Herein, we report the topical delivery of molecularly engineered antimicrobial peptides using electrospun nanofiber dressings as a carrier for the treatment of biofilms of multidrug-resistant bacteria in diabetic wounds. Molecularly engineered human cathelicidin peptide 17BIPHE2 was successfully encapsulated in the core of pluronic F127/17BIPHE2-PCL core–shell nanofibers. The in vitro release profiles of 17BIPHE2 showed an in initial burst followed by a sustained release over 4 weeks. The peptide nanofiber formulations effectively killed methicillin-resistant Staphylococcus aureus (MRSA) USA300. Similarly, the 17BIPHE2 peptide containing nanofibers could also effectively kill other bacteria including Klebsiella pneumoniae (104 to 106 CFU) and Acinetobacter baumannii (104 to 107 CFU) clinical strains in vitro without showing evident cytotoxicity to skin cells and monocytes. Importantly, 17BIPHE2-containing nanofiber dressings without debridement caused five-magnitude decreases of the MRSA USA300 CFU in a biofilm-containing chronic wound model based on type II diabetic mice. In combination with debridement, 17BIPHE2-containing nanofiber dressings could completely eliminate the biofilms, providing one possible solution to chronic wound treatment. Taken together, the biodegradable nanofiber-based wound dressings developed in this study can be utilized to effectively deliver molecularly engineered peptides to treat biofilm-containing chronic wounds. |
---|---|
AbstractList | Biofilms of multidrug-resistant bacteria in chronic wounds pose a great challenge in wound care. Herein, we report the topical delivery of molecularly engineered antimicrobial peptides using electrospun nanofiber dressings as a carrier for the treatment of biofilms of multidrug-resistant bacteria in diabetic wounds. Molecularly engineered human cathelicidin peptide 17BIPHE2 was successfully encapsulated in the core of pluronic F127/17BIPHE2-PCL core–shell nanofibers. The in vitro release profiles of 17BIPHE2 showed an in initial burst followed by a sustained release over 4 weeks. The peptide nanofiber formulations effectively killed methicillin-resistant Staphylococcus aureus (MRSA) USA300. Similarly, the 17BIPHE2 peptide containing nanofibers could also effectively kill other bacteria including Klebsiella pneumoniae (104 to 106 CFU) and Acinetobacter baumannii (104 to 107 CFU) clinical strains in vitro without showing evident cytotoxicity to skin cells and monocytes. Importantly, 17BIPHE2-containing nanofiber dressings without debridement caused five-magnitude decreases of the MRSA USA300 CFU in a biofilm-containing chronic wound model based on type II diabetic mice. In combination with debridement, 17BIPHE2-containing nanofiber dressings could completely eliminate the biofilms, providing one possible solution to chronic wound treatment. Taken together, the biodegradable nanofiber-based wound dressings developed in this study can be utilized to effectively deliver molecularly engineered peptides to treat biofilm-containing chronic wounds. Biofilms of multidrug-resistant bacteria in chronic wounds pose a great challenge in wound care. Herein, we report the topical delivery of molecularly engineered antimicrobial peptides using electrospun nanofiber dressings as a carrier for the treatment of biofilms of multidrug-resistant bacteria in diabetic wounds. Molecularly engineered human cathelicidin peptide 17BIPHE2 was successfully encapsulated in the core of pluronic F127/17BIPHE2-PCL core-shell nanofibers. The in vitro release profiles of 17BIPHE2 showed an in initial burst followed by a sustained release over 4 weeks. The peptide nanofiber formulations effectively killed methicillin-resistant Staphylococcus aureus (MRSA) USA300. Similarly, the 17BIPHE2 peptide containing nanofibers could also effectively kill other bacteria including Klebsiella pneumoniae (10 to 10 CFU) and Acinetobacter baumannii (10 to 10 CFU) clinical strains in vitro without showing evident cytotoxicity to skin cells and monocytes. Importantly, 17BIPHE2-containing nanofiber dressings without debridement caused five-magnitude decreases of the MRSA USA300 CFU in a biofilm-containing chronic wound model based on type II diabetic mice. In combination with debridement, 17BIPHE2-containing nanofiber dressings could completely eliminate the biofilms, providing one possible solution to chronic wound treatment. Taken together, the biodegradable nanofiber-based wound dressings developed in this study can be utilized to effectively deliver molecularly engineered peptides to treat biofilm-containing chronic wounds. |
Author | Jiang, Jiang Xie, Jingwei Hollins, Ronald R Lakshmaiah Narayana, Jayaram Reilly, Debra A Carlson, Mark A Wang, Hongjun Wang, Guangshun Mishra, Biswajit Su, Yajuan |
AuthorAffiliation | Department of Surgery-General Surgery, College of Medicine Department of Surgery-Plastic Surgery, College of Medicine Department of Surgery-Transplant and Mary and Dick Holland Regenerative Medicine Program, College of Medicine Department of Pathology and Microbiology, College of Medicine |
AuthorAffiliation_xml | – name: – name: Department of Surgery-Plastic Surgery, College of Medicine – name: Department of Surgery-Transplant and Mary and Dick Holland Regenerative Medicine Program, College of Medicine – name: Department of Pathology and Microbiology, College of Medicine – name: Department of Surgery-General Surgery, College of Medicine |
Author_xml | – sequence: 1 givenname: Yajuan surname: Su fullname: Su, Yajuan – sequence: 2 givenname: Hongjun surname: Wang fullname: Wang, Hongjun – sequence: 3 givenname: Biswajit surname: Mishra fullname: Mishra, Biswajit – sequence: 4 givenname: Jayaram surname: Lakshmaiah Narayana fullname: Lakshmaiah Narayana, Jayaram – sequence: 5 givenname: Jiang surname: Jiang fullname: Jiang, Jiang – sequence: 6 givenname: Debra A surname: Reilly fullname: Reilly, Debra A – sequence: 7 givenname: Ronald R surname: Hollins fullname: Hollins, Ronald R – sequence: 8 givenname: Mark A surname: Carlson fullname: Carlson, Mark A – sequence: 9 givenname: Guangshun surname: Wang fullname: Wang, Guangshun email: gwang@unmc.edu – sequence: 10 givenname: Jingwei orcidid: 0000-0002-8126-1397 surname: Xie fullname: Xie, Jingwei email: jingwei.xie@unmc.edu |
BackLink | https://www.ncbi.nlm.nih.gov/pubmed/30916573$$D View this record in MEDLINE/PubMed |
BookMark | eNqNkM1KLDEQhYMo_r-CxAeYMT-ddGepo14F_xYjLptMUnEi3UmTdAs-gy99I6PC3d1VFYdzTlHfAdoOMQBCp5TMKWH0TJs872M3rHXqtYFpnDcrQnklttA-FRWfNVyx7d-9qfbQQc5vhLBKML6L9jhRVIqa76PPBx2i8ytI-DJBzj68ZryMgze66z7wJXT-HVJR8X3swEydTkW-Cq8-ACSw-GbqdcALPa6L1XjrA36CYfQWMnYx4aLjZQI99hBGHB2-8OVe12dcnIt1isEb_BKnYPMR2nG6y3D8PQ_R8_XVcnEzu3v8c7s4v5tpLvk4s66plXJK6UpJqBtdC1lJLqmijNCqdo1YScuVM6SpjGVSMcu4E9IKypQk_BCpTa9JMecErh2S73X6aClpvwC3BXD7D-D2G3DJnmyyw7Tqwf4mf4gWg9gYvjre4pRCeeU_iv8CZcyTNA |
CitedBy_id | crossref_primary_10_1016_j_drudis_2021_03_008 crossref_primary_10_3390_polym16050664 crossref_primary_10_1016_j_ajps_2019_11_008 crossref_primary_10_1021_acsnano_0c04527 crossref_primary_10_1007_s13346_021_00932_7 crossref_primary_10_1002_adma_202208069 crossref_primary_10_1016_j_cocis_2023_101701 crossref_primary_10_3390_pharmaceutics14010006 crossref_primary_10_1002_adhm_202100135 crossref_primary_10_1016_j_ijpharm_2021_120525 crossref_primary_10_1039_D1TB00067E crossref_primary_10_3390_hygiene3030024 crossref_primary_10_1002_mabi_202300519 crossref_primary_10_3389_fmats_2023_1168616 crossref_primary_10_1021_acs_molpharmaceut_2c00918 crossref_primary_10_1002_app_52178 crossref_primary_10_1016_j_eurpolymj_2022_111490 crossref_primary_10_1021_acsinfecdis_3c00293 crossref_primary_10_1021_acs_molpharmaceut_0c00504 crossref_primary_10_3389_fmicb_2021_710199 crossref_primary_10_1002_cnma_202100349 crossref_primary_10_1016_j_apmt_2024_102073 crossref_primary_10_1371_journal_pone_0305137 crossref_primary_10_1016_j_micpath_2024_106763 crossref_primary_10_1007_s10965_022_02902_0 crossref_primary_10_1089_wound_2023_0011 crossref_primary_10_1021_acsabm_3c00731 crossref_primary_10_1016_j_ijpharm_2022_122215 crossref_primary_10_3390_nano11102716 crossref_primary_10_1016_j_bioorg_2024_107151 crossref_primary_10_1039_D1TB02617H crossref_primary_10_1016_j_jconrel_2023_02_030 crossref_primary_10_1016_j_ijpharm_2023_123169 crossref_primary_10_1039_D0TB01074J crossref_primary_10_1039_D3TB02146G crossref_primary_10_1186_s12951_021_01051_8 crossref_primary_10_1016_j_actbio_2020_03_035 crossref_primary_10_1021_acsinfecdis_1c00101 crossref_primary_10_1007_s10989_024_10613_x crossref_primary_10_1021_acs_molpharmaceut_1c00944 crossref_primary_10_1016_j_drudis_2022_04_020 crossref_primary_10_1021_acsnano_2c05812 crossref_primary_10_1111_brv_12830 |
Cites_doi | 10.1021/acs.chemrev.8b00277 10.1517/14656566.2015.1021780 10.1016/j.jconrel.2010.06.025 10.3390/ph10030058 10.1021/cm049580f 10.1016/j.polymer.2008.01.027 10.1021/cb500475y 10.1007/s11095-015-1667-5 10.1021/acs.molpharmaceut.6b00758 10.1021/mp4003442 10.1128/AAC.02554-14 10.1007/s00203-015-1148-6 10.1016/j.carbpol.2010.08.083 10.1016/j.biomaterials.2010.01.112 10.1016/j.ijantimicag.2017.01.019 10.1016/j.diabres.2008.11.030 10.1016/j.burns.2006.06.010 10.1016/j.carbpol.2012.03.080 10.1021/mp3001952 10.1371/journal.pone.0056616 10.1111/j.1524-475X.2009.00543.x 10.1021/acs.molpharmaceut.5b00560 10.1089/sur.2009.024 10.1016/j.biomaterials.2014.03.056 10.1016/j.imbio.2010.07.003 10.1021/mp200028w 10.1016/j.ijbiomac.2014.10.055 10.1021/mp500846u 10.1038/nri.2016.29 10.1088/0957-4484/16/9/082 10.2106/JBJS.I.00080 10.1111/iwj.12761 10.2174/1381612824666180327113418 10.1016/j.actbio.2011.09.009 10.1021/acs.molpharmaceut.6b00764 10.1002/adhm.201601173 10.1016/j.cbpa.2017.03.014 10.1016/j.addr.2017.09.018 10.5021/ad.2009.21.3.308 10.1111/wrr.12369 10.1021/acs.molpharmaceut.7b00093 10.1007/s10856-017-5893-8 10.1016/j.colsurfb.2003.12.004 10.1021/acsmedchemlett.5b00433 10.1016/j.jvs.2015.10.003 10.1002/anie.200902672 10.1002/mabi.200600069 10.1111/jam.12191 10.1016/j.bbamem.2015.11.003 |
ContentType | Journal Article |
DBID | CGR CUY CVF ECM EIF NPM AAYXX CITATION |
DOI | 10.1021/acs.molpharmaceut.8b01345 |
DatabaseName | Medline MEDLINE MEDLINE (Ovid) MEDLINE MEDLINE PubMed CrossRef |
DatabaseTitle | MEDLINE Medline Complete MEDLINE with Full Text PubMed MEDLINE (Ovid) CrossRef |
DatabaseTitleList | MEDLINE |
Database_xml | – sequence: 1 dbid: NPM name: PubMed url: https://proxy.k.utb.cz/login?url=http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed sourceTypes: Index Database – sequence: 2 dbid: EIF name: MEDLINE url: https://proxy.k.utb.cz/login?url=https://www.webofscience.com/wos/medline/basic-search sourceTypes: Index Database |
DeliveryMethod | fulltext_linktorsrc |
Discipline | Pharmacy, Therapeutics, & Pharmacology |
EISSN | 1543-8392 |
EndPage | 2020 |
ExternalDocumentID | 10_1021_acs_molpharmaceut_8b01345 30916573 d16790861 |
Genre | Research Support, Non-U.S. Gov't Journal Article Research Support, N.I.H., Extramural |
GroupedDBID | - 123 53G 55A 7~N AABXI ABMVS ABUCX ACGFS ACS AEESW AENEX AFEFF ALMA_UNASSIGNED_HOLDINGS AQSVZ CS3 DU5 EBS ED ED~ EJD F5P GNL H~9 IH9 JG JG~ P2P RNS ROL UI2 VF5 VG9 W1F X --- -~X 4.4 5VS ABFRP ABJNI ABQRX ADHLV AHGAQ BAANH CGR CUPRZ CUY CVF ECM EIF GGK NPM AAYXX CITATION |
ID | FETCH-LOGICAL-a363t-df8799f99a496e78a756463619120147f85b6d39fc084cd2692d23f56d5129603 |
IEDL.DBID | ACS |
ISSN | 1543-8384 |
IngestDate | Fri Aug 23 03:16:50 EDT 2024 Thu May 23 23:42:42 EDT 2024 Thu Aug 27 13:44:03 EDT 2020 |
IsPeerReviewed | true |
IsScholarly | true |
Issue | 5 |
Keywords | topical delivery electrospinning biofilms nanofibers antimicrobial peptides chronic wounds |
Language | English |
LinkModel | DirectLink |
MergedId | FETCHMERGED-LOGICAL-a363t-df8799f99a496e78a756463619120147f85b6d39fc084cd2692d23f56d5129603 |
ORCID | 0000-0002-8126-1397 |
PMID | 30916573 |
PageCount | 10 |
ParticipantIDs | crossref_primary_10_1021_acs_molpharmaceut_8b01345 pubmed_primary_30916573 acs_journals_10_1021_acs_molpharmaceut_8b01345 |
ProviderPackageCode | JG~ 55A AABXI GNL VF5 7~N VG9 W1F ACS AEESW AFEFF ABMVS ABUCX IH9 AQSVZ ED~ UI2 |
PublicationCentury | 2000 |
PublicationDate | 2019-05-06 |
PublicationDateYYYYMMDD | 2019-05-06 |
PublicationDate_xml | – month: 05 year: 2019 text: 2019-05-06 day: 06 |
PublicationDecade | 2010 |
PublicationPlace | United States |
PublicationPlace_xml | – name: United States |
PublicationTitle | Molecular pharmaceutics |
PublicationTitleAlternate | Mol. Pharmaceutics |
PublicationYear | 2019 |
Publisher | American Chemical Society |
Publisher_xml | – name: American Chemical Society |
References | ref9/cit9 ref99/cit99 ref45/cit45 ref3/cit3 ref27/cit27 ref16/cit16 ref23/cit23 ref8/cit8 ref31/cit31 ref2/cit2 ref34/cit34 ref37/cit37 ref20/cit20 ref48/cit48 ref17/cit17 ref10/cit10 ref35/cit35 ref19/cit19 ref21/cit21 ref42/cit42 ref46/cit46 ref13/cit13 ref24/cit24 ref38/cit38 ref6/cit6 ref36/cit36 ref18/cit18 ref11/cit11 ref25/cit25 ref29/cit29 ref32/cit32 ref39/cit39 ref14/cit14 ref5/cit5 ref43/cit43 ref28/cit28 ref40/cit40 ref26/cit26 ref12/cit12 ref15/cit15 ref41/cit41 ref22/cit22 ref33/cit33 ref4/cit4 ref30/cit30 ref47/cit47 ref1/cit1 ref44/cit44 ref7/cit7 |
References_xml | – ident: ref14/cit14 doi: 10.1021/acs.chemrev.8b00277 – ident: ref8/cit8 doi: 10.1517/14656566.2015.1021780 – ident: ref29/cit29 doi: 10.1016/j.jconrel.2010.06.025 – ident: ref25/cit25 doi: 10.3390/ph10030058 – ident: ref44/cit44 doi: 10.1021/cm049580f – ident: ref32/cit32 doi: 10.1016/j.polymer.2008.01.027 – ident: ref38/cit38 doi: 10.1021/cb500475y – ident: ref39/cit39 doi: 10.1007/s11095-015-1667-5 – ident: ref27/cit27 doi: 10.1021/acs.molpharmaceut.6b00758 – ident: ref33/cit33 doi: 10.1021/mp4003442 – ident: ref23/cit23 doi: 10.1128/AAC.02554-14 – ident: ref47/cit47 doi: 10.1007/s00203-015-1148-6 – ident: ref36/cit36 doi: 10.1016/j.carbpol.2010.08.083 – ident: ref16/cit16 doi: 10.1016/j.biomaterials.2010.01.112 – ident: ref6/cit6 doi: 10.1016/j.ijantimicag.2017.01.019 – ident: ref4/cit4 doi: 10.1016/j.diabres.2008.11.030 – ident: ref12/cit12 doi: 10.1016/j.burns.2006.06.010 – ident: ref35/cit35 doi: 10.1016/j.carbpol.2012.03.080 – ident: ref42/cit42 doi: 10.1021/mp3001952 – ident: ref20/cit20 doi: 10.1371/journal.pone.0056616 – ident: ref5/cit5 doi: 10.1111/j.1524-475X.2009.00543.x – ident: ref26/cit26 doi: 10.1021/acs.molpharmaceut.5b00560 – ident: ref1/cit1 doi: 10.1089/sur.2009.024 – ident: ref17/cit17 doi: 10.1016/j.biomaterials.2014.03.056 – ident: ref18/cit18 doi: 10.1016/j.imbio.2010.07.003 – ident: ref28/cit28 doi: 10.1021/mp200028w – ident: ref46/cit46 doi: 10.1016/j.ijbiomac.2014.10.055 – ident: ref10/cit10 doi: 10.1021/mp500846u – ident: ref22/cit22 doi: 10.1038/nri.2016.29 – ident: ref37/cit37 doi: 10.1088/0957-4484/16/9/082 – ident: ref3/cit3 doi: 10.2106/JBJS.I.00080 – ident: ref11/cit11 doi: 10.1111/iwj.12761 – ident: ref19/cit19 doi: 10.2174/1381612824666180327113418 – ident: ref99/cit99 doi: 10.1016/j.actbio.2011.09.009 – ident: ref45/cit45 doi: 10.1021/acs.molpharmaceut.6b00764 – ident: ref40/cit40 doi: 10.1002/adhm.201601173 – ident: ref48/cit48 doi: 10.1016/j.cbpa.2017.03.014 – ident: ref2/cit2 doi: 10.1016/j.addr.2017.09.018 – ident: ref13/cit13 doi: 10.5021/ad.2009.21.3.308 – ident: ref15/cit15 doi: 10.1111/wrr.12369 – ident: ref9/cit9 doi: 10.1021/acs.molpharmaceut.7b00093 – ident: ref34/cit34 doi: 10.1007/s10856-017-5893-8 – ident: ref43/cit43 doi: 10.1016/j.colsurfb.2003.12.004 – ident: ref24/cit24 doi: 10.1021/acsmedchemlett.5b00433 – ident: ref7/cit7 doi: 10.1016/j.jvs.2015.10.003 – ident: ref30/cit30 doi: 10.1002/anie.200902672 – ident: ref31/cit31 doi: 10.1002/mabi.200600069 – ident: ref41/cit41 doi: 10.1111/jam.12191 – ident: ref21/cit21 doi: 10.1016/j.bbamem.2015.11.003 |
SSID | ssj0024523 |
Score | 2.4861577 |
Snippet | Biofilms of multidrug-resistant bacteria in chronic wounds pose a great challenge in wound care. Herein, we report the topical delivery of molecularly... |
SourceID | crossref pubmed acs |
SourceType | Aggregation Database Index Database Publisher |
StartPage | 2011 |
SubjectTerms | Administration, Cutaneous Animals Anti-Bacterial Agents - chemistry Anti-Bacterial Agents - pharmacology Antimicrobial Cationic Peptides - chemistry Antimicrobial Cationic Peptides - pharmacology Bandages Biofilms - drug effects Cell Survival - drug effects Diabetes Mellitus, Experimental - complications Disease Models, Animal Drug Delivery Systems - methods Drug Liberation Humans Methicillin-Resistant Staphylococcus aureus - drug effects Mice Nanofibers - administration & dosage Nanofibers - chemistry Poloxamer - chemistry Polyesters - chemistry Protein Engineering Skin - drug effects Skin - microbiology Wound Infection - drug therapy Wound Infection - pathology |
Title | Nanofiber Dressings Topically Delivering Molecularly Engineered Human Cathelicidin Peptides for the Treatment of Biofilms in Chronic Wounds |
URI | http://dx.doi.org/10.1021/acs.molpharmaceut.8b01345 https://www.ncbi.nlm.nih.gov/pubmed/30916573 |
Volume | 16 |
hasFullText | 1 |
inHoldings | 1 |
isFullTextHit | |
isPrint | |
link | http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwlV1LT-MwELZYkFZ74b280ayEOJGS2okdH3lVFRKoEkFwixLbQRWlRbgcyl_gTzN2kq26HFiuIztWMl88X-KZbwg5iHQk2ywXQayUCBAhLEDcmCBkSmnjeiX5bg1X17x7G13ex_fTOu5_T_Bp-zhXtvU0coWn9S_eVuL-3UXxD7JAXSah40NnN1OBvdj3dENq4JZNop_kz5eXcqFJ2ZnQNEMyfbDpLJG0KdmpckweW6_joqXePis4fuc-lsliTT7hpELLCpkzw1Vy2KvGTo4gnRZj2SM4hN5U13qyRt5xJ0YoFuYFzn327PDBQjp6dm4eTODcDFyOB1rhqum5i-ZG8NBo8AcG4GsOB33Vx6gJPZdUo40F5M6AdkibxHcYlXDadw3FnyzgyFrFF-5cHyi7Tm47F-lZN6h7OQQ542wc6DIRUpZS5pHkRiS5iLnTKsPPRaQgkSiTuOCayVKFSaQ05ZJqysqYa8dIeMh-k_nhaGg2CUgcxqjQeZgj0HRRiDBvhxq3FqUlLZItglC0Wf0u2swfs9N25owzDshqB2wR2vg9e640Pv5n0kaFkL9TGFIvHgu2_d31d8gvfAbSp1HyXTI_fnk1e0h1xsW-h_YHIqX-zw |
link.rule.ids | 315,786,790,2782,27109,27957,27958,57093,57143 |
linkProvider | American Chemical Society |
linkToHtml | http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwnV1LbxMxEB5BkYAL70d5DhLqqRs267W9PkJLFaCpIrEVlTisdm0vikiTqk4P4S_wp5lxNo3Chcd15Nd6Zj2f7fE3AK9zl5u-qHUirdUJWYhIyG58kgprnedcSTFbw_BIDY7zjyfypIuq5LcwNIhALYV4ib9mF-i_YdnpjN-fdie9vYKP8HJ5Fa5JTftyhkV7n9c8ezKmdiOEwL0X-XV49cem2EPZsOGhNrBm9DkHt-Hr5WhjqMn33sW86dkfvxE5_t_n3IFbHRTFt0vbuQtX_PQe7IyWZRe7WK6fZoVd3MHRmuV6cR9-0rpMhtn4c9yPsbTTbwHL2RkrfbLAfT_hiA-S4nCVgZfEK_pD7zBeH2B8gTgZ2zH5UBxxiI3zAQlJI8mxXIXB46zFd2NOL34akEp2nL74hbNChQdwfPC-3BskXWaHpBZKzBPXFtqY1pg6N8rrotZSMXMZbR4JkOS6LWSjnDCtTYvcukyZzGWilcoxPlGpeAhb09nUPwY0VExk2tVpTWbnmkandT91tNBYZ7Km2Aae_6r7M0MVL92zfsXCDQVUnQK2IVupvzpbMn78TaVHS0O5rCIIiCmpxZN_7f8l3BiUw8Pq8MPRp6dwk-bDxABL9Qy25ucX_jmBoHnzIlr7L7z2B0k |
linkToPdf | http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwnV1LbxMxEB5BkSouUN6FAoOEeuqGzXptr49tQ1QerSKRit5Wu7YXRaRJVKeH8Bf408w4m0bhwuM6sr3enbHnW3tmPoC3uctNV1Q6kdbqhCxEJGQ3PkmFtc4zV1Jkazg9Uyfn-ccLedEeuHEuDE0i0EghXuLzqp65pq0w0H3H8ssp56C2p72dgo_xcnkb7kgm8mZodPxlXWtPRno3Qgk8gyLfhjd_HIq9lA0bXmoDb0a_078P5c2MY7jJ9871vO7YH78Vc_z_V9qBey0kxcOlDT2AW37yEPYHy7aLAxyuU7TCAe7jYF3tevEIftL-TAZa-yvsxZjaybeAw-mMlT9eYM-POfKDpHi6YuIl8aoMoncYrxEwZiKOR3ZEvhQHHGrjfEBC1EhyHK7C4XHa4NGIacYvA1LLtrYvfmV2qPAYzvvvh8cnScvwkFRCiXnimkIb0xhT5UZ5XVRaKq5gRj-RBExy3RSyVk6YxqZFbl2mTOYy0UjlGKeoVDyBrcl04p8BGmomMu2qtCLzc3Wt06qbOtpwrDNZXewC66BsV2go4-V71i1ZuKGAslXALmQrEyhny8off9Pp6dJYbroIAmRKavH8X5__GrYHvX75-cPZpxdwlz6HiXGWag-25lfX_iVhoXn9Khr8L_ajCcM |
openUrl | ctx_ver=Z39.88-2004&ctx_enc=info%3Aofi%2Fenc%3AUTF-8&rfr_id=info%3Asid%2Fsummon.serialssolutions.com&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Ajournal&rft.genre=article&rft.atitle=Nanofiber+Dressings+Topically+Delivering+Molecularly+Engineered+Human+Cathelicidin+Peptides+for+the+Treatment+of+Biofilms+in+Chronic+Wounds&rft.jtitle=Molecular+pharmaceutics&rft.au=Su%2C+Yajuan&rft.au=Wang%2C+Hongjun&rft.au=Mishra%2C+Biswajit&rft.au=Lakshmaiah+Narayana%2C+Jayaram&rft.date=2019-05-06&rft.pub=American+Chemical+Society&rft.issn=1543-8384&rft.eissn=1543-8392&rft.volume=16&rft.issue=5&rft.spage=2011&rft.epage=2020&rft_id=info:doi/10.1021%2Facs.molpharmaceut.8b01345&rft.externalDocID=d16790861 |
thumbnail_l | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/lc.gif&issn=1543-8384&client=summon |
thumbnail_m | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/mc.gif&issn=1543-8384&client=summon |
thumbnail_s | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/sc.gif&issn=1543-8384&client=summon |