Isotopic Labeling and Normal-Mode Analysis of a Model Green Fluorescent Protein Chromophore
Unambiguous vibrational band assignments have been made to 4‘-hydroxybenzylidene-2,3-dimethyl-imidazolinone (HBDI), a model compound of the green fluorescent protein (GFP) chromophore, with the use of isotopic labeling at positions C1, C3, N4, C5, and the bridging Cα. Vibrational spectra were collec...
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Published in | The journal of physical chemistry. B Vol. 106; no. 23; pp. 6056 - 6066 |
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Main Authors | , , |
Format | Journal Article |
Language | English |
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American Chemical Society
13.06.2002
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Abstract | Unambiguous vibrational band assignments have been made to 4‘-hydroxybenzylidene-2,3-dimethyl-imidazolinone (HBDI), a model compound of the green fluorescent protein (GFP) chromophore, with the use of isotopic labeling at positions C1, C3, N4, C5, and the bridging Cα. Vibrational spectra were collected using Raman and IR spectroscopy and ab initio normal mode calculations were performed using density functional theory (DFT) and a 6-31G** basis set. Although several reports of calculations and measurements on GFP model compounds have recently appeared, we are able to definitively assign normal modes for the first time because of the use of isotopic labeling. Specifically, in the region between 1750 and 1550 cm-1, we have identified marker bands both in Raman and IR spectra for cationic, neutral, and anionic forms of the chromophore. The Raman bands at 1744 (cation), 1697 (neutral), and 1665 cm-1 (anion) are assigned to νC O arising from the imidazolinone carbonyl group, whereas the bands at 1647 (cation), 1642 (neutral), and 1631 cm-1 (anion) are assigned to νC C for the exocyclic CC double bond. In addition, a band at 1567 (neutral) and 1556 cm-1 (anion) is assigned to a normal mode delocalized over the imidazolinone ring and exocyclic double bond. Importantly, a band at 1582 cm-1 in cationic HBDI also involves a contribution from N−H bending of the protonated imidazolinone N4−H and consequently is very sensitive to deuteration. Because the Raman spectra of neutral and anionic HBDI in H2O and D2O are virtually identical, the sensitivity of the 1582 cm-1 band in cationic HBDI to deuteration provides a means of identifying protonation of the imidazolinone ring in green fluorescent protein. These assignments are discussed with reference to the Raman spectra of GFPs obtained in an earlier study (Bell, A. F.; He, X.; Wachter, R. M.; Tonge, P. J. Biochemistry 2000, 39, 4423−4431) and are crucial for interpreting the vibrational spectra of GFPs. |
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AbstractList | Unambiguous vibrational band assignments have been made to 4‘-hydroxybenzylidene-2,3-dimethyl-imidazolinone (HBDI), a model compound of the green fluorescent protein (GFP) chromophore, with the use of isotopic labeling at positions C1, C3, N4, C5, and the bridging Cα. Vibrational spectra were collected using Raman and IR spectroscopy and ab initio normal mode calculations were performed using density functional theory (DFT) and a 6-31G** basis set. Although several reports of calculations and measurements on GFP model compounds have recently appeared, we are able to definitively assign normal modes for the first time because of the use of isotopic labeling. Specifically, in the region between 1750 and 1550 cm-1, we have identified marker bands both in Raman and IR spectra for cationic, neutral, and anionic forms of the chromophore. The Raman bands at 1744 (cation), 1697 (neutral), and 1665 cm-1 (anion) are assigned to νC O arising from the imidazolinone carbonyl group, whereas the bands at 1647 (cation), 1642 (neutral), and 1631 cm-1 (anion) are assigned to νC C for the exocyclic CC double bond. In addition, a band at 1567 (neutral) and 1556 cm-1 (anion) is assigned to a normal mode delocalized over the imidazolinone ring and exocyclic double bond. Importantly, a band at 1582 cm-1 in cationic HBDI also involves a contribution from N−H bending of the protonated imidazolinone N4−H and consequently is very sensitive to deuteration. Because the Raman spectra of neutral and anionic HBDI in H2O and D2O are virtually identical, the sensitivity of the 1582 cm-1 band in cationic HBDI to deuteration provides a means of identifying protonation of the imidazolinone ring in green fluorescent protein. These assignments are discussed with reference to the Raman spectra of GFPs obtained in an earlier study (Bell, A. F.; He, X.; Wachter, R. M.; Tonge, P. J. Biochemistry 2000, 39, 4423−4431) and are crucial for interpreting the vibrational spectra of GFPs. |
Author | He, Xiang Bell, Alasdair F Tonge, Peter J |
Author_xml | – sequence: 1 givenname: Xiang surname: He fullname: He, Xiang – sequence: 2 givenname: Alasdair F surname: Bell fullname: Bell, Alasdair F – sequence: 3 givenname: Peter J surname: Tonge fullname: Tonge, Peter J |
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Cites_doi | 10.1021/jp010052q 10.1038/nsb739 10.1016/S0009-2614(01)00938-1 10.1073/pnas.91.26.12501 10.1021/bi00056a003 10.1021/bi991634p 10.1021/bi00039a024 10.1038/373663b0 10.1073/pnas.93.24.13617 10.1111/j.1751-1097.1982.tb02651.x 10.1038/41048 10.1021/bi992675o 10.1021/jp003683d 10.1021/jp0046243 10.1038/nbt1096-1252 10.1016/S0040-4039(98)01031-4 10.1016/0014-5793(96)00149-4 10.1016/S0022-2860(00)00825-5 10.1016/S0968-0004(99)01432-2 10.1021/bi981170f 10.1016/S0959-440X(97)80153-4 10.1021/ja000032m 10.1146/annurev.biochem.67.1.509 10.1021/bi970563w 10.1016/S0301-0104(01)00344-5 10.1073/pnas.96.11.6177 10.1038/13657 10.1146/annurev.bb.23.060194.001243 10.1021/bi001733z 10.1016/S0968-0004(00)89099-4 10.1016/S0969-2126(98)00127-0 10.1021/ar00049a002 10.1073/pnas.98.2.462 10.1073/pnas.93.16.8362 10.1038/35073068 10.1038/nbt1096-1246 10.1016/0014-5793(79)80818-2 |
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References | Callender R. (jp0145560b00023/jp0145560b00023_1) 1994; 23 jp0145560b00044/jp0145560b00044_1 Wachter R. M. (jp0145560b00016/jp0145560b00016_1) 1997; 36 Pinchas S. (jp0145560b00045/jp0145560b00045_1) 1971 Devanathan S. (jp0145560b00027/jp0145560b00027_1) 1999; 38 Ormo M. (jp0145560b00006/jp0145560b00006_1) 1996; 273 Frisch M. J. (jp0145560b00038/jp0145560b00038_1) 1998 Dickson R. M. (jp0145560b00020/jp0145560b00020_1) 1997; 388 Heim R. (jp0145560b00015/jp0145560b00015_1) 1995; 373 Ward W. W. (jp0145560b00012/jp0145560b00012_1) 1982; 35 Tozzini V. (jp0145560b00034/jp0145560b00034_1) 2001; 105 Tsien R. Y. (jp0145560b00001/jp0145560b00001_1) 1998; 67 Chattoraj M. (jp0145560b00010/jp0145560b00010_1) 1996; 93 Unno M. (jp0145560b00028/jp0145560b00028_1) 2000; 122 jp0145560b00036/jp0145560b00036_1 Nielsen S. B. (jp0145560b00039/jp0145560b00039_1) 2001; 87 Kim M. (jp0145560b00025/jp0145560b00025_1) 1995; 34 Wachter R. M. (jp0145560b00017/jp0145560b00017_1) 1998; 6 Cubitt A. B. (jp0145560b00009/jp0145560b00009_1) 1995; 20 van Thor J. J. (jp0145560b00035/jp0145560b00035_1) 2002; 9 Litvinenko K. L. (jp0145560b00041/jp0145560b00041_1) 2001; 346 Esposito A. P. (jp0145560b00032/jp0145560b00032_1) 2001; 569 Heim R. (jp0145560b00008/jp0145560b00008_1) 1994; 91 Chen M. C. (jp0145560b00040/jp0145560b00040_1) 2001; 270 Kochendoerfer G. G. (jp0145560b00030/jp0145560b00030_1) 1999; 24 Shimomura O. (jp0145560b00003/jp0145560b00003_1) 1979; 104 Matz M. V. (jp0145560b00018/jp0145560b00018_1) 1999; 17 Niwa H. (jp0145560b00013/jp0145560b00013_1) 1996; 93 Weber W. (jp0145560b00043/jp0145560b00043_1) 1999; 96 van Thor J. J. (jp0145560b00021/jp0145560b00021_1) 1998; 37 Mathies R. A. (jp0145560b00029/jp0145560b00029_1) 1987; 2 Phillips G. N., Jr. (jp0145560b00042/jp0145560b00042_1) 1997; 7 Carey P. R. (jp0145560b00024/jp0145560b00024_1) 1995; 28 Yoo H. Y. (jp0145560b00031/jp0145560b00031_1) 2001; 105 Yokoe H. (jp0145560b00011/jp0145560b00011_1) 1996; 14 Cody C. W. (jp0145560b00004/jp0145560b00004_1) 1993; 32 Bell A. F. (jp0145560b00014/jp0145560b00014_1) 2000; 39 Kort R. (jp0145560b00026/jp0145560b00026_1) 1996; 382 Carey P. R. (jp0145560b00022/jp0145560b00022_1) 1982 Schellenberg P. (jp0145560b00033/jp0145560b00033_1) 2001; 105 Yarbrough D. (jp0145560b00019/jp0145560b00019_1) 2001; 98 Kojima S. (jp0145560b00002/jp0145560b00002_1) 1998; 39 Yang F. (jp0145560b00007/jp0145560b00007_1) 1996; 14 Lippincott-Schwartz J. (jp0145560b00005/jp0145560b00005_1) 2001; 2 Bell A. F. (jp0145560b00037/jp0145560b00037_1) 2001; 40 |
References_xml | – volume: 105 start-page: 5797 year: 2001 ident: jp0145560b00034/jp0145560b00034_1 publication-title: J. Phys. Chem. B doi: 10.1021/jp010052q contributor: fullname: Tozzini V. – volume: 9 start-page: 37 year: 2002 ident: jp0145560b00035/jp0145560b00035_1 publication-title: Nat. Struct. Biol. doi: 10.1038/nsb739 contributor: fullname: van Thor J. J. – volume: 346 start-page: 47 year: 2001 ident: jp0145560b00041/jp0145560b00041_1 publication-title: Chem. Phys. Lett. doi: 10.1016/S0009-2614(01)00938-1 contributor: fullname: Litvinenko K. L. – volume: 91 start-page: 12501 year: 1994 ident: jp0145560b00008/jp0145560b00008_1 publication-title: Proc. Natl. Acad. Sci. U.S.A. doi: 10.1073/pnas.91.26.12501 contributor: fullname: Heim R. – volume: 32 start-page: 1212 year: 1993 ident: jp0145560b00004/jp0145560b00004_1 publication-title: Biochemistry doi: 10.1021/bi00056a003 contributor: fullname: Cody C. W. – volume: 38 start-page: 13766 year: 1999 ident: jp0145560b00027/jp0145560b00027_1 publication-title: Biochemistry doi: 10.1021/bi991634p contributor: fullname: Devanathan S. – volume: 34 start-page: 12669 year: 1995 ident: jp0145560b00025/jp0145560b00025_1 publication-title: J. Biochemistry doi: 10.1021/bi00039a024 contributor: fullname: Kim M. – volume: 373 start-page: 663 year: 1995 ident: jp0145560b00015/jp0145560b00015_1 publication-title: Nature doi: 10.1038/373663b0 contributor: fullname: Heim R. – ident: jp0145560b00036/jp0145560b00036_1 – volume: 93 start-page: 13617 year: 1996 ident: jp0145560b00013/jp0145560b00013_1 publication-title: Proc. Natl. Acad. Sci. U.S.A. doi: 10.1073/pnas.93.24.13617 contributor: fullname: Niwa H. – volume-title: Gaussian 98, revision A.7 year: 1998 ident: jp0145560b00038/jp0145560b00038_1 contributor: fullname: Frisch M. J. – volume: 35 start-page: 803 year: 1982 ident: jp0145560b00012/jp0145560b00012_1 publication-title: Photochem. Photobiol. doi: 10.1111/j.1751-1097.1982.tb02651.x contributor: fullname: Ward W. W. – volume: 388 start-page: 355 year: 1997 ident: jp0145560b00020/jp0145560b00020_1 publication-title: Nature doi: 10.1038/41048 contributor: fullname: Dickson R. M. – volume: 39 start-page: 4423 year: 2000 ident: jp0145560b00014/jp0145560b00014_1 publication-title: J. Biochemistry doi: 10.1021/bi992675o contributor: fullname: Bell A. F. – volume: 105 start-page: 2850 year: 2001 ident: jp0145560b00031/jp0145560b00031_1 publication-title: J. Phys. Chem. B doi: 10.1021/jp003683d contributor: fullname: Yoo H. Y. – volume: 105 start-page: 5316 year: 2001 ident: jp0145560b00033/jp0145560b00033_1 publication-title: J. Phys. Chem. B doi: 10.1021/jp0046243 contributor: fullname: Schellenberg P. – volume: 87 start-page: 2281021 year: 2001 ident: jp0145560b00039/jp0145560b00039_1 publication-title: Phy. Rev. Lett. contributor: fullname: Nielsen S. B. – volume: 14 start-page: 1252 year: 1996 ident: jp0145560b00011/jp0145560b00011_1 publication-title: Nat. Biotechnol. doi: 10.1038/nbt1096-1252 contributor: fullname: Yokoe H. – volume: 39 start-page: 5239 year: 1998 ident: jp0145560b00002/jp0145560b00002_1 publication-title: Tetrahedron Lett. doi: 10.1016/S0040-4039(98)01031-4 contributor: fullname: Kojima S. – volume: 382 start-page: 73 year: 1996 ident: jp0145560b00026/jp0145560b00026_1 publication-title: J. Febs Lett. doi: 10.1016/0014-5793(96)00149-4 contributor: fullname: Kort R. – volume: 569 start-page: 25 year: 2001 ident: jp0145560b00032/jp0145560b00032_1 publication-title: J. Mol. Struct. doi: 10.1016/S0022-2860(00)00825-5 contributor: fullname: Esposito A. P. – volume: 24 start-page: 300 year: 1999 ident: jp0145560b00030/jp0145560b00030_1 publication-title: Trends Biochem. Sci. doi: 10.1016/S0968-0004(99)01432-2 contributor: fullname: Kochendoerfer G. G. – volume: 37 start-page: 16915 year: 1998 ident: jp0145560b00021/jp0145560b00021_1 publication-title: J. Biochemistry doi: 10.1021/bi981170f contributor: fullname: van Thor J. J. – volume: 7 start-page: 821 year: 1997 ident: jp0145560b00042/jp0145560b00042_1 publication-title: Curr. Opin. Struct. Biol. doi: 10.1016/S0959-440X(97)80153-4 contributor: fullname: Phillips G. N., Jr. – volume-title: Infrared Spectra of Labeled Compounds year: 1971 ident: jp0145560b00045/jp0145560b00045_1 contributor: fullname: Pinchas S. – volume: 122 start-page: 4233 year: 2000 ident: jp0145560b00028/jp0145560b00028_1 publication-title: J. Am. Chem. Soc. doi: 10.1021/ja000032m contributor: fullname: Unno M. – ident: jp0145560b00044/jp0145560b00044_1 – volume: 67 start-page: 509 year: 1998 ident: jp0145560b00001/jp0145560b00001_1 publication-title: Annu. Rev. Biochem. doi: 10.1146/annurev.biochem.67.1.509 contributor: fullname: Tsien R. Y. – volume: 36 start-page: 9759 year: 1997 ident: jp0145560b00016/jp0145560b00016_1 publication-title: J. Biochemistry doi: 10.1021/bi970563w contributor: fullname: Wachter R. M. – volume: 270 start-page: 157 year: 2001 ident: jp0145560b00040/jp0145560b00040_1 publication-title: Chem. Phys. doi: 10.1016/S0301-0104(01)00344-5 contributor: fullname: Chen M. C. – volume: 96 start-page: 6177 year: 1999 ident: jp0145560b00043/jp0145560b00043_1 publication-title: Proc. Natl. Acad. Sci. U.S.A. doi: 10.1073/pnas.96.11.6177 contributor: fullname: Weber W. – volume: 17 start-page: 969 year: 1999 ident: jp0145560b00018/jp0145560b00018_1 publication-title: Nat. Biotechnol. doi: 10.1038/13657 contributor: fullname: Matz M. V. – volume: 23 start-page: 215 year: 1994 ident: jp0145560b00023/jp0145560b00023_1 publication-title: Annu. Rev. Biophys. Biomol. Struct. doi: 10.1146/annurev.bb.23.060194.001243 contributor: fullname: Callender R. – volume: 40 start-page: 1725 year: 2001 ident: jp0145560b00037/jp0145560b00037_1 publication-title: J. Biochemistry doi: 10.1021/bi001733z contributor: fullname: Bell A. F. – volume: 20 start-page: 448 year: 1995 ident: jp0145560b00009/jp0145560b00009_1 publication-title: Trends Biochem. Sci. doi: 10.1016/S0968-0004(00)89099-4 contributor: fullname: Cubitt A. B. – volume: 6 start-page: 1267 year: 1998 ident: jp0145560b00017/jp0145560b00017_1 publication-title: J. Structure doi: 10.1016/S0969-2126(98)00127-0 contributor: fullname: Wachter R. M. – volume: 28 start-page: 8 year: 1995 ident: jp0145560b00024/jp0145560b00024_1 publication-title: J. Acc. Chem. Res. doi: 10.1021/ar00049a002 contributor: fullname: Carey P. R. – volume: 273 start-page: 1392 year: 1996 ident: jp0145560b00006/jp0145560b00006_1 publication-title: J. Science contributor: fullname: Ormo M. – volume-title: Biochemical applications of Raman and resonance Raman spectroscopies year: 1982 ident: jp0145560b00022/jp0145560b00022_1 contributor: fullname: Carey P. R. – volume: 98 start-page: 462 year: 2001 ident: jp0145560b00019/jp0145560b00019_1 publication-title: J. Proc. Natl. Acad. Sci. U. S.A. doi: 10.1073/pnas.98.2.462 contributor: fullname: Yarbrough D. – volume: 2 start-page: 59 volume-title: Resonance Raman Spectra of Polyenes and Aromatics year: 1987 ident: jp0145560b00029/jp0145560b00029_1 contributor: fullname: Mathies R. A. – volume: 93 start-page: 8362 year: 1996 ident: jp0145560b00010/jp0145560b00010_1 publication-title: Proc. Natl. Acad. Sci. U.S.A. doi: 10.1073/pnas.93.16.8362 contributor: fullname: Chattoraj M. – volume: 2 start-page: 444 year: 2001 ident: jp0145560b00005/jp0145560b00005_1 publication-title: Nat. Rev. Mol. Cell. Biol. doi: 10.1038/35073068 contributor: fullname: Lippincott-Schwartz J. – volume: 14 start-page: 1246 year: 1996 ident: jp0145560b00007/jp0145560b00007_1 publication-title: Nat. Biotechnol. doi: 10.1038/nbt1096-1246 contributor: fullname: Yang F. – volume: 104 start-page: 220 year: 1979 ident: jp0145560b00003/jp0145560b00003_1 publication-title: Febs Lett. doi: 10.1016/0014-5793(79)80818-2 contributor: fullname: Shimomura O. |
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Title | Isotopic Labeling and Normal-Mode Analysis of a Model Green Fluorescent Protein Chromophore |
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