Psoralen Photo-Cross-Linking by Triplex-Forming Oligonucleotides at Multiple Sites in the Human Rhodopsin Gene

Targeting DNA damage by triplex-forming oligonucleotides (TFOs) represents a way of modifying gene expression and structure and a possible approach to gene therapy. We have determined that this approach can deliver damage with great specificity to sites in the human gene for the G-protein-linked rec...

Full description

Saved in:
Bibliographic Details
Published inBiochemistry (Easton) Vol. 38; no. 39; pp. 12850 - 12859
Main Authors Perkins, Brian D, Wensel, Theodore G, Vasquez, Karen M, Wilson, John H
Format Journal Article
LanguageEnglish
Published United States American Chemical Society 28.09.1999
Subjects
Online AccessGet full text

Cover

Loading…
Abstract Targeting DNA damage by triplex-forming oligonucleotides (TFOs) represents a way of modifying gene expression and structure and a possible approach to gene therapy. We have determined that this approach can deliver damage with great specificity to sites in the human gene for the G-protein-linked receptor rhodopsin, mutations of which can lead to the genetic disorder autosomal dominant retinitis pigmentosa. We have introduced DNA monoadducts and interstrand cross-links at multiple target sites within the gene using TFOs with a photoactivatable psoralen group at the 5‘-end. The extent of formation of photoadducts (i.e., monoadducts and cross-links) was measured at target sites with a 5‘-ApT sequence at the triplex−duplex junction and at a target site with 5‘-ApT and 5‘-TpA sequences located four and seven nucleotides away, respectively. To improve psoralen reactivity at more distant sites, psoralen moieties were attached to TFOs with nucleotide “linkers” from two to nine nucleotides in length. High-affinity binding was maintained with linkers of up to 10 nucleotides, but affinities tended to decrease somewhat with increasing linker length due to faster dissociation kinetics. DNase I footprinting indicated little, if any, interaction between linkers and the duplex. Psoralen−TFO conjugates formed DNA cross-links with high efficiency (56−65%) at 5‘-ApT sequences located at triplex junctions. At a 5‘-ApT site four nucleotides away, the efficiency varied with linker length; a four-nucleotide linker gave the highest efficiency. Duplexes with 5‘-TpA and 5‘-ApT sites two nucleotides away, in otherwise identical sequences, were cross-linked with efficiencies of 56 and 38%, respectively. These results indicate that TFO−linker−psoralen conjugates allow simultaneous, efficient targeting of multiple sites in the human rhodopsin gene.
AbstractList Targeting DNA damage by triplex-forming oligonucleotides (TFOs) represents a way of modifying gene expression and structure and a possible approach to gene therapy. We have determined that this approach can deliver damage with great specificity to sites in the human gene for the G-protein-linked receptor rhodopsin, mutations of which can lead to the genetic disorder autosomal dominant retinitis pigmentosa. We have introduced DNA monoadducts and interstrand cross-links at multiple target sites within the gene using TFOs with a photoactivatable psoralen group at the 5'-end. The extent of formation of photoadducts (i.e., monoadducts and cross-links) was measured at target sites with a 5'-ApT sequence at the triplex-duplex junction and at a target site with 5'-ApT and 5'-TpA sequences located four and seven nucleotides away, respectively. To improve psoralen reactivity at more distant sites, psoralen moieties were attached to TFOs with nucleotide "linkers" from two to nine nucleotides in length. High-affinity binding was maintained with linkers of up to 10 nucleotides, but affinities tended to decrease somewhat with increasing linker length due to faster dissociation kinetics. DNase I footprinting indicated little, if any, interaction between linkers and the duplex. Psoralen-TFO conjugates formed DNA cross-links with high efficiency (56-65%) at 5'-ApT sequences located at triplex junctions. At a 5'-ApT site four nucleotides away, the efficiency varied with linker length; a four-nucleotide linker gave the highest efficiency. Duplexes with 5'-TpA and 5'-ApT sites two nucleotides away, in otherwise identical sequences, were cross-linked with efficiencies of 56 and 38%, respectively. These results indicate that TFO-linker-psoralen conjugates allow simultaneous, efficient targeting of multiple sites in the human rhodopsin gene.
Targeting DNA damage by triplex-forming oligonucleotides (TFOs) represents a way of modifying gene expression and structure and a possible approach to gene therapy. We have determined that this approach can deliver damage with great specificity to sites in the human gene for the G-protein-linked receptor rhodopsin, mutations of which can lead to the genetic disorder autosomal dominant retinitis pigmentosa. We have introduced DNA monoadducts and interstrand cross-links at multiple target sites within the gene using TFOs with a photoactivatable psoralen group at the 5‘-end. The extent of formation of photoadducts (i.e., monoadducts and cross-links) was measured at target sites with a 5‘-ApT sequence at the triplex−duplex junction and at a target site with 5‘-ApT and 5‘-TpA sequences located four and seven nucleotides away, respectively. To improve psoralen reactivity at more distant sites, psoralen moieties were attached to TFOs with nucleotide “linkers” from two to nine nucleotides in length. High-affinity binding was maintained with linkers of up to 10 nucleotides, but affinities tended to decrease somewhat with increasing linker length due to faster dissociation kinetics. DNase I footprinting indicated little, if any, interaction between linkers and the duplex. Psoralen−TFO conjugates formed DNA cross-links with high efficiency (56−65%) at 5‘-ApT sequences located at triplex junctions. At a 5‘-ApT site four nucleotides away, the efficiency varied with linker length; a four-nucleotide linker gave the highest efficiency. Duplexes with 5‘-TpA and 5‘-ApT sites two nucleotides away, in otherwise identical sequences, were cross-linked with efficiencies of 56 and 38%, respectively. These results indicate that TFO−linker−psoralen conjugates allow simultaneous, efficient targeting of multiple sites in the human rhodopsin gene.
Author Perkins, Brian D
Wensel, Theodore G
Vasquez, Karen M
Wilson, John H
Author_xml – sequence: 1
  givenname: Brian D
  surname: Perkins
  fullname: Perkins, Brian D
– sequence: 2
  givenname: Theodore G
  surname: Wensel
  fullname: Wensel, Theodore G
– sequence: 3
  givenname: Karen M
  surname: Vasquez
  fullname: Vasquez, Karen M
– sequence: 4
  givenname: John H
  surname: Wilson
  fullname: Wilson, John H
BackLink https://www.ncbi.nlm.nih.gov/pubmed/10504255$$D View this record in MEDLINE/PubMed
BookMark eNqF0U9LHDEYBvBQLLpaD_0CJZcWekjN38nk2C5VC6sOuj2H7EzGjc4ka5IB_fbNMiI9FHoKefLjDTzvMTjwwVsAPhL8jWBKzjZOKUwlZ-_AggiKEVdKHIAFxrhCVFX4CByn9FCuHEt-CI4IFphTIRbANylEM1gPm23IAS1jSAmtnH90_h5uXuA6ut1gn9F5iOM-uhncffBTO9iQXWcTNBleTUPeK3jnckmch3lr4eU0Gg9vt6ELu1SyC-vtB_C-N0Oyp6_nCfh9_nO9vESrm4tfy-8rZFhFMjLKSKkq0ZoK10TUFRFMmqrueqyoIb1kTPRMcdVWHeWkM7jnPVUMF0barmcn4Ms8dxfD02RT1qNLrR0G422YkpZYSsEJ-y8ksvRU47rArzNs9w1F2-tddKOJL5pgvd-CfttCsZ9eh06b0XZ_ybn2AtAMXMr2-e3dxEddSSaFXjd3-rpZ00Zc_9C3xX-evWmTfghT9KW8f3z8B3BHnhU
CitedBy_id crossref_primary_10_1081_CAR_200059973
crossref_primary_10_1089_oli_2010_0247
crossref_primary_10_1038_sj_embor_7400418
crossref_primary_10_1039_c3ob40915e
crossref_primary_10_1016_j_gene_2003_09_037
crossref_primary_10_1002_em_20557
crossref_primary_10_1021_bi7024029
crossref_primary_10_1016_j_biochi_2011_04_001
crossref_primary_10_1080_10426500500536523
crossref_primary_10_1081_NCN_120025240
crossref_primary_10_1089_oli_2006_16_196
crossref_primary_10_1562_0031_8655_2000_072_0298_BAPOPL_2_0_CO_2
crossref_primary_10_4028_www_scientific_net_AEF_38_57
crossref_primary_10_1562_0031_8655_2000_0720298BAPOPL2_0_CO2
Cites_doi 10.1016/S0021-9258(19)89479-6
10.1111/j.1751-1097.1984.tb04549.x
10.1074/jbc.271.3.1805
10.1016/S0021-9258(18)42824-4
10.1016/S0021-9258(18)37980-8
10.1016/S0968-0004(97)01158-4
10.1073/pnas.94.1.79
10.1146/annurev.bi.54.070185.005443
10.1016/0014-5793(94)00995-3
10.1074/jbc.271.50.31807
10.1074/jbc.271.24.14438
10.1021/bc00031a005
10.1042/bj3140427
10.1016/0014-5793(95)00987-K
ContentType Journal Article
Copyright Copyright © 1999 American Chemical Society
Copyright_xml – notice: Copyright © 1999 American Chemical Society
DBID BSCLL
CGR
CUY
CVF
ECM
EIF
NPM
AAYXX
CITATION
7TM
7X8
DOI 10.1021/bi9902743
DatabaseName Istex
Medline
MEDLINE
MEDLINE (Ovid)
MEDLINE
MEDLINE
PubMed
CrossRef
Nucleic Acids Abstracts
MEDLINE - Academic
DatabaseTitle MEDLINE
Medline Complete
MEDLINE with Full Text
PubMed
MEDLINE (Ovid)
CrossRef
Nucleic Acids Abstracts
MEDLINE - Academic
DatabaseTitleList MEDLINE
Nucleic Acids Abstracts
MEDLINE - Academic

Database_xml – sequence: 1
  dbid: NPM
  name: PubMed
  url: https://proxy.k.utb.cz/login?url=http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed
  sourceTypes: Index Database
– sequence: 2
  dbid: EIF
  name: MEDLINE
  url: https://proxy.k.utb.cz/login?url=https://www.webofscience.com/wos/medline/basic-search
  sourceTypes: Index Database
DeliveryMethod fulltext_linktorsrc
Discipline Anatomy & Physiology
Chemistry
EISSN 1520-4995
EndPage 12859
ExternalDocumentID 10_1021_bi9902743
10504255
ark_67375_TPS_NPT2P5NB_R
c385950977
Genre Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, P.H.S
Journal Article
GrantInformation_xml – fundername: NEI NIH HHS
  grantid: EY11731
– fundername: NEI NIH HHS
  grantid: T32EY07102
GroupedDBID -
.K2
02
08R
186
23N
3O-
4.4
53G
55
55A
5GY
5RE
5VS
7~N
85S
AABXI
AAYJJ
ABDTD
ABFLS
ABMVS
ABOCM
ABPTK
ABUCX
ABUFD
ACGFS
ACJ
ACNCT
ACS
ADBIT
AEESW
AENEX
AETEA
AFEFF
AFFDN
AFFNX
AFMIJ
AIDAL
ALMA_UNASSIGNED_HOLDINGS
ANTXH
AQSVZ
BAANH
CS3
D0L
DU5
DZ
EBS
ED
ED~
EJD
F5P
G8K
GJ
GNL
IH9
IHE
JG
JG~
K2
K78
KM
L7B
LG6
MVM
NHB
OHT
P2P
ROL
TN5
UI2
UQL
VF5
VG9
VQA
W1F
WH7
X
X7M
YXE
YZZ
ZA5
ZE2
ZGI
ZXP
---
-DZ
-~X
.55
.GJ
6TJ
ABFRP
ABJNI
ABQRX
ADHLV
AEQTP
AGHSJ
AGXLV
AHGAQ
BSCLL
GGK
XOL
YYP
ZCA
~02
~KM
CGR
CUPRZ
CUY
CVF
ECM
EIF
NPM
AAYXX
CITATION
7TM
ABDPE
7X8
ID FETCH-LOGICAL-a361t-a9a77965ca60815861537a68df092a1f7335f3949c6d241da0f4f29305371cdf3
IEDL.DBID ACS
ISSN 0006-2960
IngestDate Fri Oct 25 07:25:27 EDT 2024
Fri Oct 25 22:10:47 EDT 2024
Thu Sep 26 18:02:06 EDT 2024
Sat Sep 28 08:39:11 EDT 2024
Wed Jan 17 04:51:16 EST 2024
Thu Feb 04 21:43:07 EST 2021
IsPeerReviewed true
IsScholarly true
Issue 39
Language English
LinkModel DirectLink
MergedId FETCHMERGED-LOGICAL-a361t-a9a77965ca60815861537a68df092a1f7335f3949c6d241da0f4f29305371cdf3
Notes This work was funded by grants from the Texas Advanced Technology Program (004949-803) and the NIH (EY11731). B.D.P. is supported by a training grant from NIH (T32 EY07102).
ark:/67375/TPS-NPT2P5NB-R
istex:4C0B4EC22F6068DA318FB10A215F64D60F2D614A
ObjectType-Article-2
SourceType-Scholarly Journals-1
ObjectType-Feature-1
content type line 23
ObjectType-Article-1
ObjectType-Feature-2
PMID 10504255
PQID 17425808
PQPubID 23462
PageCount 10
ParticipantIDs proquest_miscellaneous_70775413
proquest_miscellaneous_17425808
crossref_primary_10_1021_bi9902743
pubmed_primary_10504255
istex_primary_ark_67375_TPS_NPT2P5NB_R
acs_journals_10_1021_bi9902743
ProviderPackageCode JG~
55A
AABXI
GNL
VF5
7~N
ACJ
VG9
W1F
ANTXH
ACS
AEESW
AFEFF
.K2
ABMVS
ABUCX
IH9
BAANH
AQSVZ
ED~
UI2
PublicationCentury 1900
PublicationDate 1999-09-28
PublicationDateYYYYMMDD 1999-09-28
PublicationDate_xml – month: 09
  year: 1999
  text: 1999-09-28
  day: 28
PublicationDecade 1990
PublicationPlace United States
PublicationPlace_xml – name: United States
PublicationTitle Biochemistry (Easton)
PublicationTitleAlternate Biochemistry
PublicationYear 1999
Publisher American Chemical Society
Publisher_xml – name: American Chemical Society
References Svinarchuk F. (bi9902743b00064/bi9902743b00064_1) 1996
Takasugi M. (bi9902743b00050/bi9902743b00050_1) 1991
Shi Y. B. (bi9902743b00051/bi9902743b00051_1) 1987
Perkins B. D. (bi9902743b00035/bi9902743b00035_1) 1998
Raha M. (bi9902743b00039/bi9902743b00039_1) 1998
Gamper H. (bi9902743b00048/bi9902743b00048_1) 1984; 40
Berson E. L. (bi9902743b00003/bi9902743b00003_1) 1996
Boyer V. (bi9902743b00030/bi9902743b00030_1) 1988
Macaulay V. M. (bi9902743b00054/bi9902743b00054_1) 1995; 372
Averbeck D. (bi9902743b00052/bi9902743b00052_1) 1985
Vasquez K. M. (bi9902743b00036/bi9902743b00036_1) 1995
Gia O. (bi9902743b00031/bi9902743b00031_1) 1992
McShan W. M. (bi9902743b00014/bi9902743b00014_1) 1992; 267
Sage E. (bi9902743b00032/bi9902743b00032_1) 1987
Cimino G. D. (bi9902743b00028/bi9902743b00028_1) 1985; 54
Bates P. J. (bi9902743b00057/bi9902743b00057_1) 1995
Chandler S. P. (bi9902743b00040/bi9902743b00040_1) 1996
Tu G. C. (bi9902743b00018/bi9902743b00018_1) 1995; 270
Orson F. M. (bi9902743b00012/bi9902743b00012_1) 1991
Nathans J. (bi9902743b00001/bi9902743b00001_1) 1992
Rouet P. (bi9902743b00033/bi9902743b00033_1) 1994
de Bizemont T. (bi9902743b00042/bi9902743b00042_1) 1996
Cassidy S. A. (bi9902743b00044/bi9902743b00044_1) 1994
Olivas W. M. (bi9902743b00067/bi9902743b00067_1) 1936
Sambrook J. (bi9902743b00038/bi9902743b00038_1) 1989
Zhen W. P. (bi9902743b00047/bi9902743b00047_1) 1986
Giovannangeli C. (bi9902743b00055/bi9902743b00055_1) 1997; 94
Degols G. (bi9902743b00053/bi9902743b00053_1) 1994; 269
Barawkar D. A. (bi9902743b00061/bi9902743b00061_1) 1998
Musso M. (bi9902743b00058/bi9902743b00058_1) 1996
Sandor Z. (bi9902743b00022/bi9902743b00022_1) 1994
Aggarwal B. B. (bi9902743b00021/bi9902743b00021_1) 1996
Sargent R. G. (bi9902743b00034/bi9902743b00034_1) 1997
Svinarchuk F. (bi9902743b00010/bi9902743b00010_1) 1996
Okada T. (bi9902743b00016/bi9902743b00016_1) 1994
Brown P. M. (bi9902743b00045/bi9902743b00045_1) 1996; 314
Rao V. R. (bi9902743b00002/bi9902743b00002_1) 1996; 25
Scaggiante B. (bi9902743b00015/bi9902743b00015_1) 1994; 352
Brown P. M. (bi9902743b00070/bi9902743b00070_1) 1998
Guieysse A. L. (bi9902743b00056/bi9902743b00056_1) 1996
Nara H. (bi9902743b00068/bi9902743b00068_1) 1995; 6
Kochetkova M. (bi9902743b00011/bi9902743b00011_1) 1996; 271
Ing N. H. (bi9902743b00008/bi9902743b00008_1) 1993
Thomas T. J. (bi9902743b00017/bi9902743b00017_1) 1995
Rosenfeld P. J. (bi9902743b00006/bi9902743b00006_1) 1992
Faucon B. (bi9902743b00059/bi9902743b00059_1) 1996
Postel E. H. (bi9902743b00013/bi9902743b00013_1) 1991
Espinas M. L. (bi9902743b00062/bi9902743b00062_1) 1996; 271
Tsukahara S. (bi9902743b00069/bi9902743b00069_1) 1997
Humphries M. M. (bi9902743b00005/bi9902743b00005_1) 1997
Vasquez K. M. (bi9902743b00046/bi9902743b00046_1) 1996
Porumb H. (bi9902743b00019/bi9902743b00019_1) 1996
Brown P. M. (bi9902743b00063/bi9902743b00063_1) 1996; 319
Gasparro F. P. (bi9902743b00049/bi9902743b00049_1) 1994
Kandimalla E. R. (bi9902743b00060/bi9902743b00060_1) 1995
Jayasena S. D. (bi9902743b00041/bi9902743b00041_1) 1992
Wang G. (bi9902743b00024/bi9902743b00024_1) 1996
Washbrook E. (bi9902743b00043/bi9902743b00043_1) 1994
Majumdar A. (bi9902743b00025/bi9902743b00025_1) 1998
Abbreviations TFO (bi9902743n00001/bi9902743n00001_1)
Rininsland F. (bi9902743b00020/bi9902743b00020_1) 1997
Heckenlively J. R. (bi9902743b00004/bi9902743b00004_1) 1988
Vasquez K. M. (bi9902743b00007/bi9902743b00007_1) 1998; 23
Faruqi A. F. (bi9902743b00026/bi9902743b00026_1) 1996
Kovacs A. (bi9902743b00009/bi9902743b00009_1) 1996; 271
Sandor Z. (bi9902743b00027/bi9902743b00027_1) 1995; 1263
Faruqi A. F. (bi9902743b00065/bi9902743b00065_1) 1997
Xodo L. E. (bi9902743b00037/bi9902743b00037_1) 1997; 248
Esposito F. (bi9902743b00029/bi9902743b00029_1) 1988; 263
Lacoste J. (bi9902743b00066/bi9902743b00066_1) 1997
Wang G. (bi9902743b00023/bi9902743b00023_1) 1995
References_xml – volume-title: Cancer Res. 56, 5156−5164
  year: 1996
  ident: bi9902743b00021/bi9902743b00021_1
  contributor:
    fullname: Aggarwal B. B.
– volume-title: Nucleic Acids Res. 22
  year: 1994
  ident: bi9902743b00022/bi9902743b00022_1
  contributor:
    fullname: Sandor Z.
– volume: 1263
  year: 1995
  ident: bi9902743b00027/bi9902743b00027_1
  publication-title: Biochim. Biophys. Acta
  contributor:
    fullname: Sandor Z.
– volume-title: Biochemistry 37, 11315−11322
  year: 1998
  ident: bi9902743b00035/bi9902743b00035_1
  contributor:
    fullname: Perkins B. D.
– volume-title: Photochem. Photobiol. 67, 289−294
  year: 1998
  ident: bi9902743b00039/bi9902743b00039_1
  contributor:
    fullname: Raha M.
– volume-title: Mol. Cell. Biol. 17, 267−277
  year: 1997
  ident: bi9902743b00034/bi9902743b00034_1
  contributor:
    fullname: Sargent R. G.
– volume: 269
  year: 1994
  ident: bi9902743b00053/bi9902743b00053_1
  publication-title: J. Biol. Chem.
  doi: 10.1016/S0021-9258(19)89479-6
  contributor:
    fullname: Degols G.
– volume-title: Mol. Cell. Biol. 15, 1759−1768
  year: 1995
  ident: bi9902743b00023/bi9902743b00023_1
  contributor:
    fullname: Wang G.
– volume: 40
  start-page: 34
  year: 1984
  ident: bi9902743b00048/bi9902743b00048_1
  publication-title: Photochem. Photobiol.
  doi: 10.1111/j.1751-1097.1984.tb04549.x
  contributor:
    fullname: Gamper H.
– volume: 319
  year: 1996
  ident: bi9902743b00063/bi9902743b00063_1
  publication-title: Biochem. J.
  contributor:
    fullname: Brown P. M.
– volume-title: Nucleic Acids Res. 24, 1136−1143
  year: 1996
  ident: bi9902743b00042/bi9902743b00042_1
  contributor:
    fullname: de Bizemont T.
– volume-title: Nucleic Acids Res. 25, 633−640
  year: 1997
  ident: bi9902743b00065/bi9902743b00065_1
  contributor:
    fullname: Faruqi A. F.
– volume-title: Nucleic Acids Res. 24, 4210−4216
  year: 1996
  ident: bi9902743b00056/bi9902743b00056_1
  contributor:
    fullname: Guieysse A. L.
– volume: 271
  year: 1996
  ident: bi9902743b00009/bi9902743b00009_1
  publication-title: J. Biol. Chem.
  doi: 10.1074/jbc.271.3.1805
  contributor:
    fullname: Kovacs A.
– volume: 267
  year: 1992
  ident: bi9902743b00014/bi9902743b00014_1
  publication-title: J. Biol. Chem.
  doi: 10.1016/S0021-9258(18)42824-4
  contributor:
    fullname: McShan W. M.
– volume: 263
  year: 1988
  ident: bi9902743b00029/bi9902743b00029_1
  publication-title: J. Biol. Chem.
  doi: 10.1016/S0021-9258(18)37980-8
  contributor:
    fullname: Esposito F.
– volume-title: Molecular Cloning:  A Laboratory Manual
  year: 1989
  ident: bi9902743b00038/bi9902743b00038_1
  contributor:
    fullname: Sambrook J.
– volume-title: Biochemistry 35, 15038−15048
  year: 1996
  ident: bi9902743b00040/bi9902743b00040_1
  contributor:
    fullname: Chandler S. P.
– volume: 23
  start-page: 9
  year: 1998
  ident: bi9902743b00007/bi9902743b00007_1
  publication-title: Trends Biochem. Sci.
  doi: 10.1016/S0968-0004(97)01158-4
  contributor:
    fullname: Vasquez K. M.
– volume-title: Cancer Res. 56, 515−522
  year: 1996
  ident: bi9902743b00019/bi9902743b00019_1
  contributor:
    fullname: Porumb H.
– volume: 94
  start-page: 84
  year: 1997
  ident: bi9902743b00055/bi9902743b00055_1
  publication-title: Proc. Natl. Acad. Sci. U.S.A.
  doi: 10.1073/pnas.94.1.79
  contributor:
    fullname: Giovannangeli C.
– volume-title: Nucleic Acids Res. 25
  year: 1997
  ident: bi9902743b00066/bi9902743b00066_1
  contributor:
    fullname: Lacoste J.
– volume-title: triplex-forming oligonucleotide
  ident: bi9902743n00001/bi9902743n00001_1
  contributor:
    fullname: Abbreviations TFO
– volume-title: Proc. Natl. Acad. Sci. U.S.A. 93, 4526−4528
  year: 1996
  ident: bi9902743b00003/bi9902743b00003_1
  contributor:
    fullname: Berson E. L.
– volume-title: Proc. Natl. Acad. Sci. U.S.A. 88, 8227−8231
  year: 1991
  ident: bi9902743b00013/bi9902743b00013_1
  contributor:
    fullname: Postel E. H.
– volume-title: Nucleic Acids Res. 23, 3594−3599
  year: 1995
  ident: bi9902743b00017/bi9902743b00017_1
  contributor:
    fullname: Thomas T. J.
– volume-title: Biochemistry 27, 3011−3018
  year: 1988
  ident: bi9902743b00030/bi9902743b00030_1
  contributor:
    fullname: Boyer V.
– volume-title: Nucleic Acids Res. 15, 6843−6854
  year: 1987
  ident: bi9902743b00051/bi9902743b00051_1
  contributor:
    fullname: Shi Y. B.
– volume-title: Nucleic Acids Res. 24, 3858−3865
  year: 1996
  ident: bi9902743b00064/bi9902743b00064_1
  contributor:
    fullname: Svinarchuk F.
– volume-title: Biochemistry 34, 7243−7251
  year: 1995
  ident: bi9902743b00036/bi9902743b00036_1
  contributor:
    fullname: Vasquez K. M.
– volume-title: Science 271, 802−805
  year: 1996
  ident: bi9902743b00024/bi9902743b00024_1
  contributor:
    fullname: Wang G.
– volume-title: Biochemistry 35, 10712−10719
  year: 1996
  ident: bi9902743b00046/bi9902743b00046_1
  contributor:
    fullname: Vasquez K. M.
– volume-title: Biochemistry 26, 3307−3314
  year: 1987
  ident: bi9902743b00032/bi9902743b00032_1
  contributor:
    fullname: Sage E.
– volume: 54
  year: 1985
  ident: bi9902743b00028/bi9902743b00028_1
  publication-title: Annu. Rev. Biochem.
  doi: 10.1146/annurev.bi.54.070185.005443
  contributor:
    fullname: Cimino G. D.
– volume-title: Biochemistry 31, 11818−11822
  year: 1992
  ident: bi9902743b00031/bi9902743b00031_1
  contributor:
    fullname: Gia O.
– volume: 352
  year: 1994
  ident: bi9902743b00015/bi9902743b00015_1
  publication-title: FEBS Lett.
  doi: 10.1016/0014-5793(94)00995-3
  contributor:
    fullname: Scaggiante B.
– volume: 271
  year: 1996
  ident: bi9902743b00062/bi9902743b00062_1
  publication-title: J. Biol. Chem.
  doi: 10.1074/jbc.271.50.31807
  contributor:
    fullname: Espinas M. L.
– volume-title: Nucleic Acids Res. 20, 5279−5288
  year: 1992
  ident: bi9902743b00041/bi9902743b00041_1
  contributor:
    fullname: Jayasena S. D.
– volume-title: Biochemistry 31, 4923−4931
  year: 1992
  ident: bi9902743b00001/bi9902743b00001_1
  contributor:
    fullname: Nathans J.
– volume-title: Proc. Natl. Acad. Sci. U.S.A. 94, 5854−5859
  year: 1997
  ident: bi9902743b00020/bi9902743b00020_1
  contributor:
    fullname: Rininsland F.
– volume: 248
  year: 1997
  ident: bi9902743b00037/bi9902743b00037_1
  publication-title: Eur. J. Biochem.
  contributor:
    fullname: Xodo L. E.
– volume-title: III (1995) Nucleic Acids Res. 23
  year: 1936
  ident: bi9902743b00067/bi9902743b00067_1
  contributor:
    fullname: Olivas W. M.
– volume-title: Nucleic Acids Res. 19, 3435−3441
  year: 1991
  ident: bi9902743b00012/bi9902743b00012_1
  contributor:
    fullname: Orson F. M.
– volume: 271
  year: 1996
  ident: bi9902743b00011/bi9902743b00011_1
  publication-title: J. Biol. Chem.
  doi: 10.1074/jbc.271.24.14438
  contributor:
    fullname: Kochetkova M.
– volume-title: Biochemistry 37, 16139−16151
  year: 1998
  ident: bi9902743b00070/bi9902743b00070_1
  contributor:
    fullname: Brown P. M.
– volume-title: Growth Factors 11, 259−270
  year: 1994
  ident: bi9902743b00016/bi9902743b00016_1
  contributor:
    fullname: Okada T.
– volume-title: Nucleic Acids Res. 22, 2845−2852
  year: 1994
  ident: bi9902743b00049/bi9902743b00049_1
  contributor:
    fullname: Gasparro F. P.
– volume: 25
  year: 1996
  ident: bi9902743b00002/bi9902743b00002_1
  publication-title: Annu. Rev. Biophys. Biomol. Struct.
  contributor:
    fullname: Rao V. R.
– volume-title: Nucleic Acids Res. 24, 4924−4932
  year: 1996
  ident: bi9902743b00058/bi9902743b00058_1
  contributor:
    fullname: Musso M.
– volume-title: Mutat. Res. 151, 217−233
  year: 1985
  ident: bi9902743b00052/bi9902743b00052_1
  contributor:
    fullname: Averbeck D.
– volume: 270
  year: 1995
  ident: bi9902743b00018/bi9902743b00018_1
  publication-title: J. Biol. Chem.
  contributor:
    fullname: Tu G. C.
– volume-title: Biochemistry 33, 15338−15347
  year: 1994
  ident: bi9902743b00044/bi9902743b00044_1
  contributor:
    fullname: Cassidy S. A.
– volume-title: Proc. Natl. Acad. Sci. U.S.A. 88, 5602−5606
  year: 1991
  ident: bi9902743b00050/bi9902743b00050_1
  contributor:
    fullname: Takasugi M.
– volume-title: Biochem. Biophys. Res. Commun. 233, 742−747
  year: 1997
  ident: bi9902743b00069/bi9902743b00069_1
  contributor:
    fullname: Tsukahara S.
– volume-title: Nucleic Acids Res. 23, 4283−4289
  year: 1995
  ident: bi9902743b00057/bi9902743b00057_1
  contributor:
    fullname: Bates P. J.
– volume-title: Mol. Cell. Biol. 14, 8096−8106
  year: 1994
  ident: bi9902743b00033/bi9902743b00033_1
  contributor:
    fullname: Rouet P.
– volume-title: Proc. Natl. Acad. Sci. U.S.A. 95, 11047−11052
  year: 1998
  ident: bi9902743b00061/bi9902743b00061_1
  contributor:
    fullname: Barawkar D. A.
– volume-title: Nucleic Acids Res. 23, 4510−4517
  year: 1995
  ident: bi9902743b00060/bi9902743b00060_1
  contributor:
    fullname: Kandimalla E. R.
– volume-title: Mol. Cell. Biol. 16, 6820−6828
  year: 1996
  ident: bi9902743b00026/bi9902743b00026_1
  contributor:
    fullname: Faruqi A. F.
– volume-title: Biochemistry 25, 6598−6603
  year: 1986
  ident: bi9902743b00047/bi9902743b00047_1
  contributor:
    fullname: Zhen W. P.
– year: 1988
  ident: bi9902743b00004/bi9902743b00004_1
  publication-title: Retinitis Pigmentosa, J. B. Lippincott, Philadelphia.
  contributor:
    fullname: Heckenlively J. R.
– volume-title: Nucleic Acids Res. 22, 3977−3982
  year: 1994
  ident: bi9902743b00043/bi9902743b00043_1
  contributor:
    fullname: Washbrook E.
– volume: 6
  start-page: 61
  year: 1995
  ident: bi9902743b00068/bi9902743b00068_1
  publication-title: Bioconjugate Chem.
  doi: 10.1021/bc00031a005
  contributor:
    fullname: Nara H.
– volume-title: Nat. Genet. 20, 212−214
  year: 1998
  ident: bi9902743b00025/bi9902743b00025_1
  contributor:
    fullname: Majumdar A.
– volume-title: Nat. Genet. 1, 209−213
  year: 1992
  ident: bi9902743b00006/bi9902743b00006_1
  contributor:
    fullname: Rosenfeld P. J.
– volume-title: Nucleic Acids Res. 24, 3181−3188
  year: 1996
  ident: bi9902743b00059/bi9902743b00059_1
  contributor:
    fullname: Faucon B.
– volume: 314
  year: 1996
  ident: bi9902743b00045/bi9902743b00045_1
  publication-title: Biochem. J.
  doi: 10.1042/bj3140427
  contributor:
    fullname: Brown P. M.
– volume: 372
  year: 1995
  ident: bi9902743b00054/bi9902743b00054_1
  publication-title: FEBS Lett.
  doi: 10.1016/0014-5793(95)00987-K
  contributor:
    fullname: Macaulay V. M.
– volume-title: Nat. Genet. 15, 216−219
  year: 1997
  ident: bi9902743b00005/bi9902743b00005_1
  contributor:
    fullname: Humphries M. M.
– volume-title: Nucleic Acids Res. 21, 2789−2796
  year: 1993
  ident: bi9902743b00008/bi9902743b00008_1
  contributor:
    fullname: Ing N. H.
– volume-title: Nucleic Acids Res. 24, 295−302
  year: 1996
  ident: bi9902743b00010/bi9902743b00010_1
  contributor:
    fullname: Svinarchuk F.
SSID ssj0004074
Score 1.7504141
Snippet Targeting DNA damage by triplex-forming oligonucleotides (TFOs) represents a way of modifying gene expression and structure and a possible approach to gene...
SourceID proquest
crossref
pubmed
istex
acs
SourceType Aggregation Database
Index Database
Publisher
StartPage 12850
SubjectTerms Base Sequence
DNA Adducts
DNA Footprinting
Furocoumarins - chemistry
Humans
Kinetics
Oligonucleotides
Oligonucleotides - chemistry
Photochemistry
Psoralen
Rhodopsin - genetics
Title Psoralen Photo-Cross-Linking by Triplex-Forming Oligonucleotides at Multiple Sites in the Human Rhodopsin Gene
URI http://dx.doi.org/10.1021/bi9902743
https://api.istex.fr/ark:/67375/TPS-NPT2P5NB-R/fulltext.pdf
https://www.ncbi.nlm.nih.gov/pubmed/10504255
https://search.proquest.com/docview/17425808
https://search.proquest.com/docview/70775413
Volume 38
hasFullText 1
inHoldings 1
isFullTextHit
isPrint
link http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwhV3db9MwED-N7QFeYGzACmNYgPbmkS_HzmMpVBOCUq2dtLfIdpytGjhTk0obfz1nJxlDUHiNLvLHnX2_851_BnjL0IUEZZBRxWJGkzKQVMSFoqJQ6C5NHDPlq3wn6fFp8umMnW3AmzUZ_Ch8pxa4YWLsFN-DrQj9oYuwhqPZr8uPQUe1jKFxhHi8pw-6-6tzPbr-zfVsuVm8Xo8rvX8ZP4IP_S2dtqzk8mjVqCP940_Sxn91fRsedviSDFuDeAwbxu7A7tBibP39hhwSX_Hpj9J34P6of-1tF-y0dnf1jUWJqqnoyA2Bfm5fViDqhsyX7kj-mo4rVz1zTr5-W5xX1rEhV82iMDWRDfnSVSeSGQLZmiwsQXhJfJ6AnFxgAHxV4zfHdP0ETscf56Nj2r3FQGWchg2VmeQ8S5mWKYIIJhxO5DIVBeo5kmHJUatlnCWZTgsEBYUMyqR0doBioS7K-Cls2sqaPSBShyYTQgbSJIniTHKJKE2rgGudobscwAEqK-_WUp37NHkU5rezOYDXvR7zq5aT429Ch17DtxJyeemK2DjL59NZPpnOoymbvM9PBvCqN4Ec59zlS6Q11Qob5rihiUCsl-CeQDDExp61tnOnP8xth-z5_wbzAh60TBAZjcQ-bDbLlXmJOKdRB97OfwKNC_Qo
link.rule.ids 315,786,790,2782,27107,27955,27956,57091,57141
linkProvider American Chemical Society
linkToHtml http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwjV1LT9wwELZaONBLH9DH9gEWQtxM83LsHGHV1dIuy4oNEjfLdhy6onXQJitBfz1jJwu0ApVrNInt8cTzjWf8GaEdCi4kKIOMKBpTkpSBJDwuFOGFAndp4pgqX-U7ToenyfczetbR5LizMNCJGr5U-yT-HbtA-FXNYN2EECp-jlYpAy_nYFB_encGMugYlyFCjgCWL1mE7r_qPJCu__JAq06ZV4_DS-9mBq_a-4p8B311ycXeolF7-s8_3I1PG8Fr9LJDm3i_NY836Jmx62hj30Kk_fsa72Jf_-k31tfRWn9599sGspPandw3FiSqpiJ9NxIyau9ZwOoa53O3QX9FBpWrpTnHx79m55V13MhVMytMjWWDj7paRTwFWFvjmcUANrHPGuCTnxAOX9bwzPFev0Wng295f0i6mxmIjNOwITKTjGUp1TIFSEG5Q41MpryAWY9kWDKY4zLOkkynBUCEQgZlUjqrALFQF2X8Dq3YypoPCEsdmoxzGUiTJIpRySRgNq0CpnUGzrOHNkGZovuzauGT5lEobrXZQ9vL6RSXLUPHQ0K7fqJvJeT8wpW0MSryyVSMJ3k0oeMDcdJDW0tLEKBzlz2R1lQLaJjB8sYD_rgE83SCITT2vjWhe_2hbnGkH_83mC20NsyPRmJ0OP7xCb1oOSIyEvHPaKWZL8wXQECN2vSmfwOI8PyT
linkToPdf http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwjV3dT9swELc2kDZe9gEb6z7AmibezPLl2HnsulVsYyWiReLNsh0HKphTNakE_PWcnbRj09D2Gl0S23f2_c53_hmhDxRcSFAGGVE0piQpA0l4XCjCCwXu0sQxVb7Kd5QenCTfTulpFyi6szDQiBq-VPskvpvVs6LsGAbCj2oKayeEUfFDtE5ZmLiJ2B-Mf52DDDrWZYiSI4DmSyahu686L6Tr37zQuhvQq_shpnc1w6foaNVIX2Fysb9o1L6--YO_8f978Qw96VAn7rdm8hw9MHYTbfUtRNw_r_Ee9nWgfoN9Ez0eLO-A20I2r90JfmNBomoqMnC9IYftfQtYXePJ3G3UX5Fh5WpqzvDR5fSsso4juWqmhamxbPCPrmYRjwHe1nhqMYBO7LMH-PgcwuJZDc8c__ULdDL8MhkckO6GBiLjNGyIzCRjWUq1TAFaUO7QI5MpL0D7kQxLBrou4yzJdFoAVChkUCalsw4QC3VRxi_Rmq2seYWw1KHJOJeBNEmiGJVMAnbTKmBaZ-BEe2gHBlR0M6wWPnkehWI1mj30fqlSMWuZOv4mtOeVvZKQ8wtX2saomORjMconUU5Hn8RxD-0urUHAmLssirSmWsCPGSxzPOD3SzBPKxjCz7ZbM7rTHuoWSfr6X53ZRY_yz0Nx-HX0_Q3aaKkiMhLxt2itmS_MOwBCjdrx1n8Luy__DQ
openUrl ctx_ver=Z39.88-2004&ctx_enc=info%3Aofi%2Fenc%3AUTF-8&rfr_id=info%3Asid%2Fsummon.serialssolutions.com&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Ajournal&rft.genre=article&rft.atitle=Psoralen+photo-cross-linking+by+triplex-forming+oligonucleotides+at+multiple+sites+in+the+human+rhodopsin+gene&rft.jtitle=Biochemistry+%28Easton%29&rft.au=Perkins%2C+B+D&rft.au=Wensel%2C+T+G&rft.au=Vasquez%2C+K+M&rft.au=Wilson%2C+J+H&rft.date=1999-09-28&rft.issn=0006-2960&rft.volume=38&rft.issue=39&rft.spage=12850&rft_id=info:doi/10.1021%2Fbi9902743&rft_id=info%3Apmid%2F10504255&rft.externalDocID=10504255
thumbnail_l http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/lc.gif&issn=0006-2960&client=summon
thumbnail_m http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/mc.gif&issn=0006-2960&client=summon
thumbnail_s http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/sc.gif&issn=0006-2960&client=summon