Bacterial Derived Carbohydrates Bind Cyr1 and Trigger Hyphal Growth in Candida albicans
The dimorphic yeast Candida albicans is the most common pathogenic fungus found in humans. While this species is normally commensal, a morphological switch from budding yeast to filamentous hyphae allows the fungi to invade epithelial cells and cause infections. The phenotypic change is controlled b...
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Published in | ACS infectious diseases Vol. 4; no. 1; pp. 53 - 58 |
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Main Authors | , , , |
Format | Journal Article |
Language | English |
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American Chemical Society
12.01.2018
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Abstract | The dimorphic yeast Candida albicans is the most common pathogenic fungus found in humans. While this species is normally commensal, a morphological switch from budding yeast to filamentous hyphae allows the fungi to invade epithelial cells and cause infections. The phenotypic change is controlled by the adenylyl cyclase, Cyr1. Interestingly, this protein contains a leucine-rich repeat (LRR) domain, which is commonly found in innate immune receptors from plants and animals. A functional and pure LRR domain was obtained in high yields from E. coli expression. Utilizing a surface plasmon resonance assay, the LRR was found to bind diverse bacterial derived carbohydrates with high affinity. This domain is capable of binding fragments of peptidoglycan, a carbohydrate polymer component of the bacterial cell wall, as well as anthracyclines produced by Streptomyces, leading to hyphae formation. These findings add another dimension to the human microbiome, taking into account yeast–bacteria interactions that occur in the host. |
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AbstractList | The
dimorphic yeast
Candida albicans
is the most common
pathogenic fungus found in humans. While this species is normally
commensal, a morphological switch from budding yeast to filamentous
hyphae allows the fungi to invade epithelial cells and cause infections.
The phenotypic change is controlled by the adenylyl cyclase, Cyr1.
Interestingly, this protein contains a leucine-rich repeat (LRR) domain,
which is commonly found in innate immune receptors from plants and
animals. A functional and pure LRR domain was obtained in high yields
from
E. coli
expression. Utilizing a surface
plasmon resonance assay, the LRR was found to bind diverse bacterial
derived carbohydrates with high affinity. This domain is capable of
binding fragments of peptidoglycan, a carbohydrate polymer component
of the bacterial cell wall, as well as anthracyclines produced by
Streptomyces
, leading to hyphae formation. These findings
add another dimension to the human microbiome, taking into account
yeast–bacteria interactions that occur in the host. The dimorphic yeast Candida albicans is the most common pathogenic fungus found in humans. While this species is normally commensal, a morphological switch from budding yeast to filamentous hyphae allows the fungi to invade epithelial cells and cause infections. The phenotypic change is controlled by the adenylyl cyclase, Cyr1. Interestingly, this protein contains a leucine-rich repeat (LRR) domain, which is commonly found in innate immune receptors from plants and animals. A functional and pure LRR domain was obtained in high yields from E. coli expression. Utilizing a surface plasmon resonance assay, the LRR was found to bind diverse bacterial derived carbohydrates with high affinity. This domain is capable of binding fragments of peptidoglycan, a carbohydrate polymer component of the bacterial cell wall, as well as anthracyclines produced by Streptomyces, leading to hyphae formation. These findings add another dimension to the human microbiome, taking into account yeast-bacteria interactions that occur in the host. |
Author | Wykoff, Dennis D Grimes, Catherine L Mashayekh, Siavash Burch, Jason M |
AuthorAffiliation | Department of Chemistry and Biochemistry Department of Biological Sciences Department of Biology University of Delaware |
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Author_xml | – sequence: 1 givenname: Jason M surname: Burch fullname: Burch, Jason M organization: University of Delaware – sequence: 2 givenname: Siavash surname: Mashayekh fullname: Mashayekh, Siavash organization: University of Delaware – sequence: 3 givenname: Dennis D surname: Wykoff fullname: Wykoff, Dennis D organization: Department of Biology – sequence: 4 givenname: Catherine L orcidid: 0000-0002-0586-2879 surname: Grimes fullname: Grimes, Catherine L email: cgrimes@udel.edu organization: University of Delaware |
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Keywords | peptidoglycan leucine-rich-repeat domain hyphae formation anthracycline C. albicans |
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Snippet | The dimorphic yeast Candida albicans is the most common pathogenic fungus found in humans. While this species is normally commensal, a morphological switch... The dimorphic yeast Candida albicans is the most common pathogenic fungus found in humans. While this species is normally commensal, a morphological switch... |
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SubjectTerms | Bacteria - metabolism Candida albicans - physiology Carbohydrate Metabolism Carbohydrates Hyphae - growth & development Hyphae - metabolism Letter Mitochondrial Proteins - metabolism Protein Binding |
Title | Bacterial Derived Carbohydrates Bind Cyr1 and Trigger Hyphal Growth in Candida albicans |
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