Ultrasensitive Amplification-Free Quantification of a Methyl CpG-Rich Cancer Biomarker by Single-Molecule Kinetic Fingerprinting

• Published in: Analytical chemistry (Washington) Vol. 96; no. 43; pp. 17209 - 17216
• Main Authors: Dai, Liuhan, Johnson-Buck, Alexander, Laird, Peter W., Tewari, Muneesh, Walter, Nils G.
• Format: Journal Article
• Language: English
• Published: United States American Chemical Society 29.10.2024
• Subjects: Amino acids; analytical chemistry; Biomarkers; Biomarkers, Tumor - blood; Biomarkers, Tumor - genetics; Bisulfite; bisulfites; blood; Cancer; Chain branching; CpG Islands; Cytosine; Deoxyribonucleic acid; detection limit; DNA; DNA fingerprinting; DNA Methylation; DNA microarrays; Epigenetics; Gene sequencing; Genetic fingerprinting; Humans; Kinetics; Limit of Detection; microarray technology; Neoplasms - genetics; Nucleotide sequence; Polymerase chain reaction; Promoter Regions, Genetic; Sulfites - chemistry; Transaminase; Transaminases - genetics; Transaminases - metabolism
• ISSN: 0003-2700; 1520-6882; 1520-6882
• DOI: 10.1021/acs.analchem.4c03002

The most well-studied epigenetic marker in humans is the 5-methyl modification of cytosine in DNA, which has great potential as a disease biomarker. Currently, quantification of DNA methylation relies heavily on bisulfite conversion followed by PCR amplification and NGS or microarray analysis. PCR i...