Capillary Liquid Chromatographic and Automated MALDI-TOF Mass Spectrometric Analysis of Complex Carbohydrate Mixtures

Reproducible microbore reversed-phase HPLC methodology has been developed, suitable for the analysis of carbohydrates derivatized with 2-aminoacridone. The high extinction coefficient of this label at around 260 nm, combined with the use of a new diode array microflow cell, has allowed detection at...

Full description

Saved in:
Bibliographic Details
Published inAnalytical chemistry (Washington) Vol. 70; no. 18; pp. 3840 - 3844
Main Authors Grimm, Rudi, Birrell, Helen, Ross, Gordon, Charlwood, Joanne, Camilleri, Patrick
Format Journal Article
LanguageEnglish
Published Washington, DC American Chemical Society 15.09.1998
Subjects
Analytical, structural and metabolic biochemistry
Biological and medical sciences
Carbohydrates
Chemistry
Fundamental and applied biological sciences. Psychology
Miscellaneous
Other biological molecules
Scientific imaging
HPLC chromatography
Carbohydrate
Mass spectrometry
Mixture
Analytical method
Online AccessGet full text

Cover

More Information
Summary:Reproducible microbore reversed-phase HPLC methodology has been developed, suitable for the analysis of carbohydrates derivatized with 2-aminoacridone. The high extinction coefficient of this label at around 260 nm, combined with the use of a new diode array microflow cell, has allowed detection at femtomole levels. Analytes were also robotically collected onto MALDI targets, and mass spectrometric information was acquired for carbohydrate present at low picomole levels. This technology was successfully applied to mixtures of linear and branched oligosaccharides, including sialylated species.
Bibliography:ark:/67375/TPS-C6MN571P-F
istex:8D683BE6778B4D9128A7399535FA17F54219513A
ISSN:0003-2700
1520-6882
DOI:10.1021/ac980235h