Characterization of Citrullination Sites in Neutrophils and Mast Cells Activated by Ionomycin via Integration of Mass Spectrometry and Machine Learning

• Published in: Journal of proteome research Vol. 20; no. 6; pp. 3150 - 3164
• Main Authors: Chaerkady, Raghothama, Zhou, Yebin, Delmar, Jared A, Weng, Shao Huan Samuel, Wang, Junmin, Awasthi, Shivangi, Sims, Dorothy, Bowen, Michael A, Yu, Wen, Cazares, Lisa H, Sims, Gary P, Hess, Sonja
• Format: Journal Article
• Language: English
• Published: United States American Chemical Society 04.06.2021
• Subjects: ionomycin; citrullination; mast cell; mass spectrometry; deimination; protein-arginine deiminases; neutral loss; machine learning; neutrophil

Citrullination is an important post-translational modification implicated in many diseases including rheumatoid arthritis (RA), Alzheimer’s disease, and cancer. Neutrophil and mast cells have different expression profiles for protein-arginine deiminases (PADs), and ionomycin-induced activation makes...