Novel Elements of the Chondrocyte Stress Response Identified Using an in Vitro Model of Mouse Cartilage Degradation

The destruction of articular cartilage in osteoarthritis involves chondrocyte dysfunction and imbalanced extracellular matrix (ECM) homeostasis. Pro-inflammatory cytokines such as interleukin-1α (IL-1α) contribute to osteoarthritis pathophysiology, but the effects of IL-1α on chondrocytes within the...

Full description

Saved in:
Bibliographic Details
Published inJournal of proteome research Vol. 15; no. 3; pp. 1033 - 1050
Main Authors Wilson, Richard, Golub, Suzanne B, Rowley, Lynn, Angelucci, Constanza, Karpievitch, Yuliya V, Bateman, John F, Fosang, Amanda J
Format Journal Article
LanguageEnglish
Published United States American Chemical Society 04.03.2016
Subjects
Animals
Cartilage, Articular - metabolism
Cartilage, Articular - pathology
Cells, Cultured
Chondrocytes - metabolism
Interleukin-1alpha - physiology
Mice, Inbred C57BL
Proteome - metabolism
Stress, Physiological
chondrocyte
osteoarthritis
quantitative proteomics
oxidative stress
cartilage
Online AccessGet full text

Cover

Abstract The destruction of articular cartilage in osteoarthritis involves chondrocyte dysfunction and imbalanced extracellular matrix (ECM) homeostasis. Pro-inflammatory cytokines such as interleukin-1α (IL-1α) contribute to osteoarthritis pathophysiology, but the effects of IL-1α on chondrocytes within their tissue microenvironment have not been fully evaluated. To redress this we used label-free quantitative proteomics to analyze the chondrocyte response to IL-1α within a native cartilage ECM. Mouse femoral heads were cultured with and without IL-1α, and both the tissue proteome and proteins released into the media were analyzed. New elements of the chondrocyte response to IL-1α related to cellular stress included markers for protein misfolding (Armet, Creld2, and Hyou1), enzymes involved in glutathione biosynthesis and regeneration (Gstp1, Gsto1, and Gsr), and oxidative stress proteins (Prdx2, Txn, Atox1, Hmox1, and Vnn1). Other proteins previously not associated with the IL-1α response in cartilage included ECM components (Smoc2, Kera, and Crispld1) and cysteine proteases (cathepsin Z and legumain), while chondroadherin and cartilage-derived C-type lectin (Clec3a) were identified as novel products of IL-1α-induced cartilage degradation. This first proteome-level view of the cartilage IL-1α response identified candidate biomarkers of cartilage destruction and novel targets for therapeutic intervention in osteoarthritis.
AbstractList The destruction of articular cartilage in osteoarthritis involves chondrocyte dysfunction and imbalanced extracellular matrix (ECM) homeostasis. Pro-inflammatory cytokines such as interleukin-1α (IL-1α) contribute to osteoarthritis pathophysiology, but the effects of IL-1α on chondrocytes within their tissue microenvironment have not been fully evaluated. To redress this we used label-free quantitative proteomics to analyze the chondrocyte response to IL-1α within a native cartilage ECM. Mouse femoral heads were cultured with and without IL-1α, and both the tissue proteome and proteins released into the media were analyzed. New elements of the chondrocyte response to IL-1α related to cellular stress included markers for protein misfolding (Armet, Creld2, and Hyou1), enzymes involved in glutathione biosynthesis and regeneration (Gstp1, Gsto1, and Gsr), and oxidative stress proteins (Prdx2, Txn, Atox1, Hmox1, and Vnn1). Other proteins previously not associated with the IL-1α response in cartilage included ECM components (Smoc2, Kera, and Crispld1) and cysteine proteases (cathepsin Z and legumain), while chondroadherin and cartilage-derived C-type lectin (Clec3a) were identified as novel products of IL-1α-induced cartilage degradation. This first proteome-level view of the cartilage IL-1α response identified candidate biomarkers of cartilage destruction and novel targets for therapeutic intervention in osteoarthritis.
Author Bateman, John F
Wilson, Richard
Angelucci, Constanza
Golub, Suzanne B
Karpievitch, Yuliya V
Fosang, Amanda J
Rowley, Lynn
AuthorAffiliation Department of Pediatrics
Department of Biochemistry and Molecular Biology
Centre of Excellence in Plant Energy Biology
Royal Children’s Hospital
University of Melbourne
University of Western Australia and Harry Perkins Institute of Medical Research
Murdoch Childrens Research Institute
University of Tasmania
School of Physical Sciences
Central Science Laboratory
AuthorAffiliation_xml – name: Royal Children’s Hospital
– name: Murdoch Childrens Research Institute
– name: School of Physical Sciences
– name: University of Melbourne
– name: University of Western Australia and Harry Perkins Institute of Medical Research
– name: Centre of Excellence in Plant Energy Biology
– name: University of Tasmania
– name: Central Science Laboratory
– name: Department of Pediatrics
– name: Department of Biochemistry and Molecular Biology
Author_xml – sequence: 1
  givenname: Richard
  surname: Wilson
  fullname: Wilson, Richard
  email: richard.wilson@utas.edu.au
– sequence: 2
  givenname: Suzanne B
  surname: Golub
  fullname: Golub, Suzanne B
– sequence: 3
  givenname: Lynn
  surname: Rowley
  fullname: Rowley, Lynn
– sequence: 4
  givenname: Constanza
  surname: Angelucci
  fullname: Angelucci, Constanza
– sequence: 5
  givenname: Yuliya V
  surname: Karpievitch
  fullname: Karpievitch, Yuliya V
– sequence: 6
  givenname: John F
  surname: Bateman
  fullname: Bateman, John F
– sequence: 7
  givenname: Amanda J
  surname: Fosang
  fullname: Fosang, Amanda J
BackLink https://www.ncbi.nlm.nih.gov/pubmed/26794603$$D View this record in MEDLINE/PubMed
BookMark eNqFkMtOwzAQRS0E4lH4BJCXbFo8MU6cJSqvSjwkXtvIjifFKLGL7SLx9xha2LIaL869Mz57ZNN5h4QcApsAK-BEtXHytgg-oR9wIjQDALFBdkFwMeY1qzZ_37LmO2QvxjfGQFSMb5Odoqzq05LxXRLv_Af29KLHAV2K1Hc0vSKdvnpngm8_E9LHFDBG-oBx4V1EOjOZtJ1FQ5-jdXOqHLWOvtgUPL31Jtflllu_zOxUhWR7NUd6jvOgjErWu32y1ak-4sF6jsjz5cXT9Hp8c381m57djBUXkMYgsZbAKiV022EpdVfmm1HIknccCtlKMFArEDXnVVUajVprI7Upsp_i1PAROV71Zk3vS4ypGWxsse-Vw3xdA1XFpOSlLDIqVmgbfIwBu2YR7KDCZwOs-fbdZN_Nn-9m7TvnjtYrlnpA85f6FZwBWAE_eb8MLv_4n9IvClyTSg
CitedBy_id crossref_primary_10_18632_oncotarget_24634
crossref_primary_10_1371_journal_pone_0161513
crossref_primary_10_1016_j_gep_2017_10_004
crossref_primary_10_1016_j_cbd_2017_02_005
crossref_primary_10_1074_jbc_M117_818930
crossref_primary_10_1002_pmic_201600051
crossref_primary_10_1016_j_mcn_2021_103627
crossref_primary_10_1080_03008207_2019_1665035
crossref_primary_10_1016_j_joca_2019_06_007
crossref_primary_10_3389_fimmu_2023_1103097
crossref_primary_10_1074_mcp_RA120_001998
crossref_primary_10_1186_s12864_018_4517_0
crossref_primary_10_1007_s00395_020_0784_4
crossref_primary_10_1111_nph_15843
crossref_primary_10_1016_j_matbio_2016_12_004
crossref_primary_10_1097_BOR_0000000000000460
crossref_primary_10_1096_fj_202101149R
crossref_primary_10_3389_fendo_2023_1117414
crossref_primary_10_1113_JP274970
crossref_primary_10_1093_humupd_dmaa016
crossref_primary_10_32604_biocell_2021_011220
crossref_primary_10_1093_ibd_izz179
crossref_primary_10_1302_2046_3758_107_BJR_2019_0251_R4
crossref_primary_10_1371_journal_pbio_3000382
crossref_primary_10_1111_cea_13224
crossref_primary_10_1080_08977194_2019_1570926
Cites_doi 10.1007/978-3-0348-7442-7_18
10.1021/pr200331k
10.1074/mcp.M800181-MCP200
10.1038/nature03417
10.1093/hmg/ddt383
10.1186/ar4415
10.1021/pr101065j
10.3389/fncel.2014.00213
10.1002/jcb.21963
10.1161/HYPERTENSIONAHA.111.189571
10.1016/j.joca.2013.01.014
10.1038/nprot.2010.179
10.1093/bioinformatics/btg455
10.1007/s12192-010-0193-y
10.1002/1529-0131(199807)41:7<1275::AID-ART19>3.0.CO;2-T
10.1002/art.33388
10.1016/S0006-2952(03)00543-4
10.3109/1354750X.2013.810667
10.1002/art.20567
10.2337/db13-0788
10.1074/jbc.M112.443010
10.1016/j.joca.2009.12.002
10.1016/j.cbpa.2012.11.025
10.1038/cr.2014.4
10.1021/pr501024p
10.1186/ar4423
10.1002/art.23120
10.1002/art.24654
10.1074/jbc.M112.444810
10.1248/bpb.b14-00825
10.1371/journal.pone.0021800
10.3892/ijmm.2015.2158
10.1038/nprot.2008.211
10.1016/S0014-5793(00)02110-4
10.1016/0167-4838(94)90171-6
10.1002/art.23789
10.1084/jem.182.6.2097
10.1002/art.37900
10.1074/mcp.M000014-MCP201
10.1038/nbt.1511
10.2174/138945010791011956
10.1016/j.matbio.2008.07.007
10.1021/pr400661g
10.2174/138945009788488404
10.1002/art.23654
10.1016/j.jprot.2011.02.017
10.1128/MCB.24.16.7214-7224.2004
10.1002/dvdy.24131
10.1186/s13075-014-0441-0
10.1242/jcs.128033
10.1371/journal.pone.0024600
10.1038/nrrheum.2014.162
10.1016/j.matbio.2014.08.012
10.1002/art.27265
10.1042/BST20140092
10.1002/art.34420
10.1111/j.1365-2613.2010.00758.x
10.1371/journal.pgen.1005505
10.1074/mcp.M111.014159
10.1177/1759720X12467868
10.1016/j.celrep.2014.09.006
10.1107/S1399004714022767
10.1093/nar/gki456
10.1016/j.jaci.2015.01.045
10.1002/jcp.20261
10.1136/annrheumdis-2013-204763
10.1182/blood-2007-12-129080
10.1074/mcp.M113.031591
10.1002/art.34453
10.1016/j.berh.2011.11.010
10.1016/S0161-5890(01)00113-4
10.1089/ars.2007.1782
10.1093/bioinformatics/18.1.207
10.1182/blood-2010-09-304931
10.1002/humu.21631
10.1016/j.yexmp.2014.04.007
10.1016/S0167-4781(99)00087-1
ContentType Journal Article
Copyright Copyright © 2016 American Chemical Society
Copyright_xml – notice: Copyright © 2016 American Chemical Society
DBID CGR
CUY
CVF
ECM
EIF
NPM
AAYXX
CITATION
7X8
DOI 10.1021/acs.jproteome.5b01115
DatabaseName Medline
MEDLINE
MEDLINE (Ovid)
MEDLINE
MEDLINE
PubMed
CrossRef
MEDLINE - Academic
DatabaseTitle MEDLINE
Medline Complete
MEDLINE with Full Text
PubMed
MEDLINE (Ovid)
CrossRef
MEDLINE - Academic
DatabaseTitleList MEDLINE

MEDLINE - Academic
Database_xml – sequence: 1
  dbid: NPM
  name: PubMed
  url: https://proxy.k.utb.cz/login?url=http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed
  sourceTypes: Index Database
– sequence: 2
  dbid: EIF
  name: MEDLINE
  url: https://proxy.k.utb.cz/login?url=https://www.webofscience.com/wos/medline/basic-search
  sourceTypes: Index Database
DeliveryMethod fulltext_linktorsrc
Discipline Chemistry
EISSN 1535-3907
EndPage 1050
ExternalDocumentID 10_1021_acs_jproteome_5b01115
26794603
b002698663
Genre Research Support, Non-U.S. Gov't
Journal Article
GroupedDBID -
53G
55A
5GY
7~N
AABXI
ABMVS
ABUCX
ACGFS
ACS
AEESW
AENEX
AFEFF
ALMA_UNASSIGNED_HOLDINGS
AQSVZ
CS3
DU5
EBS
ED
ED~
EJD
F5P
GNL
IH9
IHE
JG
JG~
P2P
RNS
ROL
UI2
VF5
VG9
W1F
ZA5
---
4.4
5VS
AAHBH
ABJNI
ABQRX
ADHLV
AHGAQ
BAANH
CGR
CUPRZ
CUY
CVF
ECM
EIF
GGK
NPM
AAYXX
CITATION
7X8
ID FETCH-LOGICAL-a351t-18e98107a5bcfe68bf6460e5863f3128c81d19a15933776dbebbbd8bd202124d3
IEDL.DBID ACS
ISSN 1535-3893
IngestDate Fri Aug 16 20:38:15 EDT 2024
Fri Dec 06 02:19:21 EST 2024
Sat Sep 28 08:04:52 EDT 2024
Thu Aug 27 13:42:06 EDT 2020
IsPeerReviewed true
IsScholarly true
Issue 3
Keywords chondrocyte
osteoarthritis
quantitative proteomics
oxidative stress
cartilage
Language English
LinkModel DirectLink
MergedId FETCHMERGED-LOGICAL-a351t-18e98107a5bcfe68bf6460e5863f3128c81d19a15933776dbebbbd8bd202124d3
Notes ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
PMID 26794603
PQID 1770883682
PQPubID 23479
PageCount 18
ParticipantIDs proquest_miscellaneous_1770883682
crossref_primary_10_1021_acs_jproteome_5b01115
pubmed_primary_26794603
acs_journals_10_1021_acs_jproteome_5b01115
ProviderPackageCode JG~
55A
AABXI
GNL
VF5
7~N
VG9
W1F
ACS
AEESW
AFEFF
ABMVS
ABUCX
IH9
AQSVZ
ED~
UI2
PublicationCentury 2000
PublicationDate 2016-03-04
PublicationDateYYYYMMDD 2016-03-04
PublicationDate_xml – month: 03
  year: 2016
  text: 2016-03-04
  day: 04
PublicationDecade 2010
PublicationPlace United States
PublicationPlace_xml – name: United States
PublicationTitle Journal of proteome research
PublicationTitleAlternate J. Proteome Res
PublicationYear 2016
Publisher American Chemical Society
Publisher_xml – name: American Chemical Society
References ref9/cit9
ref45/cit45
ref3/cit3
ref27/cit27
ref63/cit63
ref56/cit56
ref16/cit16
ref52/cit52
ref23/cit23
ref8/cit8
ref31/cit31
ref59/cit59
ref2/cit2
ref77/cit77
ref34/cit34
ref71/cit71
ref37/cit37
ref20/cit20
ref48/cit48
ref60/cit60
ref74/cit74
ref17/cit17
ref10/cit10
ref35/cit35
ref53/cit53
ref19/cit19
ref21/cit21
ref42/cit42
ref46/cit46
ref49/cit49
ref13/cit13
ref61/cit61
ref75/cit75
ref67/cit67
ref24/cit24
ref38/cit38
ref50/cit50
ref64/cit64
ref54/cit54
ref6/cit6
ref36/cit36
ref18/cit18
ref65/cit65
ref11/cit11
ref25/cit25
ref29/cit29
ref72/cit72
ref76/cit76
ref32/cit32
ref39/cit39
ref14/cit14
ref57/cit57
ref5/cit5
ref51/cit51
ref43/cit43
ref28/cit28
ref40/cit40
ref68/cit68
ref26/cit26
ref55/cit55
ref73/cit73
ref69/cit69
ref12/cit12
ref15/cit15
ref62/cit62
ref66/cit66
ref41/cit41
ref58/cit58
ref22/cit22
ref33/cit33
ref4/cit4
ref30/cit30
ref47/cit47
ref1/cit1
ref44/cit44
ref70/cit70
ref7/cit7
References_xml – ident: ref76/cit76
  doi: 10.1007/978-3-0348-7442-7_18
– ident: ref20/cit20
  doi: 10.1021/pr200331k
– ident: ref17/cit17
  doi: 10.1074/mcp.M800181-MCP200
– ident: ref13/cit13
  doi: 10.1038/nature03417
– ident: ref63/cit63
  doi: 10.1093/hmg/ddt383
– ident: ref59/cit59
  doi: 10.1186/ar4415
– ident: ref32/cit32
  doi: 10.1021/pr101065j
– ident: ref67/cit67
  doi: 10.3389/fncel.2014.00213
– ident: ref73/cit73
  doi: 10.1002/jcb.21963
– ident: ref47/cit47
  doi: 10.1161/HYPERTENSIONAHA.111.189571
– ident: ref22/cit22
  doi: 10.1016/j.joca.2013.01.014
– ident: ref28/cit28
  doi: 10.1038/nprot.2010.179
– ident: ref33/cit33
  doi: 10.1093/bioinformatics/btg455
– ident: ref44/cit44
  doi: 10.1007/s12192-010-0193-y
– ident: ref50/cit50
  doi: 10.1002/1529-0131(199807)41:7<1275::AID-ART19>3.0.CO;2-T
– ident: ref11/cit11
  doi: 10.1002/art.33388
– ident: ref45/cit45
  doi: 10.1016/S0006-2952(03)00543-4
– ident: ref10/cit10
  doi: 10.3109/1354750X.2013.810667
– ident: ref74/cit74
  doi: 10.1002/art.20567
– ident: ref55/cit55
  doi: 10.2337/db13-0788
– ident: ref71/cit71
  doi: 10.1074/jbc.M112.443010
– ident: ref70/cit70
  doi: 10.1016/j.joca.2009.12.002
– ident: ref77/cit77
  doi: 10.1016/j.cbpa.2012.11.025
– ident: ref39/cit39
  doi: 10.1038/cr.2014.4
– ident: ref9/cit9
  doi: 10.1021/pr501024p
– ident: ref14/cit14
  doi: 10.1186/ar4423
– ident: ref16/cit16
  doi: 10.1002/art.23120
– ident: ref24/cit24
  doi: 10.1002/art.24654
– ident: ref38/cit38
  doi: 10.1074/jbc.M112.444810
– ident: ref64/cit64
  doi: 10.1248/bpb.b14-00825
– ident: ref35/cit35
  doi: 10.1371/journal.pone.0021800
– ident: ref61/cit61
  doi: 10.3892/ijmm.2015.2158
– ident: ref36/cit36
  doi: 10.1038/nprot.2008.211
– ident: ref53/cit53
  doi: 10.1016/S0014-5793(00)02110-4
– ident: ref52/cit52
  doi: 10.1016/0167-4838(94)90171-6
– ident: ref19/cit19
  doi: 10.1002/art.23789
– ident: ref51/cit51
  doi: 10.1084/jem.182.6.2097
– ident: ref15/cit15
  doi: 10.1002/art.37900
– ident: ref26/cit26
  doi: 10.1074/mcp.M000014-MCP201
– ident: ref31/cit31
  doi: 10.1038/nbt.1511
– ident: ref3/cit3
  doi: 10.2174/138945010791011956
– ident: ref29/cit29
  doi: 10.1016/j.matbio.2008.07.007
– ident: ref30/cit30
  doi: 10.1021/pr400661g
– ident: ref8/cit8
  doi: 10.2174/138945009788488404
– ident: ref40/cit40
  doi: 10.1002/art.23654
– ident: ref18/cit18
  doi: 10.1016/j.jprot.2011.02.017
– ident: ref48/cit48
  doi: 10.1128/MCB.24.16.7214-7224.2004
– ident: ref58/cit58
  doi: 10.1002/dvdy.24131
– ident: ref23/cit23
  doi: 10.1186/s13075-014-0441-0
– ident: ref41/cit41
  doi: 10.1242/jcs.128033
– ident: ref43/cit43
  doi: 10.1371/journal.pone.0024600
– ident: ref6/cit6
  doi: 10.1038/nrrheum.2014.162
– ident: ref7/cit7
  doi: 10.1016/j.matbio.2014.08.012
– ident: ref21/cit21
  doi: 10.1002/art.27265
– ident: ref49/cit49
  doi: 10.1042/BST20140092
– ident: ref12/cit12
  doi: 10.1002/art.34420
– ident: ref60/cit60
  doi: 10.1111/j.1365-2613.2010.00758.x
– ident: ref62/cit62
  doi: 10.1371/journal.pgen.1005505
– ident: ref27/cit27
  doi: 10.1074/mcp.M111.014159
– ident: ref5/cit5
  doi: 10.1177/1759720X12467868
– ident: ref75/cit75
  doi: 10.1016/j.celrep.2014.09.006
– ident: ref57/cit57
  doi: 10.1107/S1399004714022767
– ident: ref34/cit34
  doi: 10.1093/nar/gki456
– ident: ref54/cit54
  doi: 10.1016/j.jaci.2015.01.045
– ident: ref68/cit68
  doi: 10.1002/jcp.20261
– ident: ref1/cit1
  doi: 10.1136/annrheumdis-2013-204763
– ident: ref42/cit42
  doi: 10.1182/blood-2007-12-129080
– ident: ref25/cit25
  doi: 10.1074/mcp.M113.031591
– ident: ref2/cit2
  doi: 10.1002/art.34453
– ident: ref4/cit4
  doi: 10.1016/j.berh.2011.11.010
– ident: ref46/cit46
  doi: 10.1016/S0161-5890(01)00113-4
– ident: ref66/cit66
  doi: 10.1089/ars.2007.1782
– ident: ref37/cit37
  doi: 10.1093/bioinformatics/18.1.207
– ident: ref56/cit56
  doi: 10.1182/blood-2010-09-304931
– ident: ref65/cit65
  doi: 10.1002/humu.21631
– ident: ref72/cit72
  doi: 10.1016/j.yexmp.2014.04.007
– ident: ref69/cit69
  doi: 10.1016/S0167-4781(99)00087-1
SSID ssj0015703
Score 2.3581216
Snippet The destruction of articular cartilage in osteoarthritis involves chondrocyte dysfunction and imbalanced extracellular matrix (ECM) homeostasis....
SourceID proquest
crossref
pubmed
acs
SourceType Aggregation Database
Index Database
Publisher
StartPage 1033
SubjectTerms Animals
Cartilage, Articular - metabolism
Cartilage, Articular - pathology
Cells, Cultured
Chondrocytes - metabolism
Interleukin-1alpha - physiology
Mice, Inbred C57BL
Proteome - metabolism
Stress, Physiological
Title Novel Elements of the Chondrocyte Stress Response Identified Using an in Vitro Model of Mouse Cartilage Degradation
URI http://dx.doi.org/10.1021/acs.jproteome.5b01115
https://www.ncbi.nlm.nih.gov/pubmed/26794603
https://search.proquest.com/docview/1770883682
Volume 15
hasFullText 1
inHoldings 1
isFullTextHit
isPrint
link http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwlV3PT90wDLYYO7DL2BiDB9sUpJ0mtby2adIcUQdCk4YQjIlblaSu-PFoJ9o3afz1OMkr0jQ9bbtWqdXaTvxFtj8DfBR2qpXIMVLIZcTrRkWaNzpShE6kVUnD0Vf5nojjC_7lMr9cgf0lGfw02de2j288aUF3h3Fu3HD0_Bk8TyVdwR0WKs-f0gaOTioQpOaRi8Rjy84yMS4k2f73kLQEZ_p4c7QOp2PXTigzuY3ng4ntw58kjv_6K6_g5QJ7soPgLK9hBdsNWCvHkW9voD_pfuKMHYaS8p51DSN8yMqrztEa2F8DsnPfW8LOQmktstDo2xCQZb76gOmWXbfs-_Vw3zE3aG3mpHzt5rS2dG46owOMfXYUFWGa0yZcHB1-K4-jxVSGSGd5MkRJgaqgS6POjW1QFKYRXEwxL0TWZBTtLCHgRGmCSVkmpagNGmPqwtSpY5PndfYWVtuuxW1gqq4t7f8UHUd8wWmp1IWRZopkXGXMBD6RyqrFruornzBPk8o_HPVYLfQ4gXi0YvUjMHX87YW90dYV6dklSnSLpI8qkZIO30wU6QS2ghM8iUyFo-SfZjv_82278IKAlvC1a_wdrA73c3xPYGYwH7wDPwL8HfIj
link.rule.ids 314,780,784,2765,27076,27924,27925,56738,56788
linkProvider American Chemical Society
linkToHtml http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwlV1LT9xADLYoHOiF0ifbQjtIPVXKNo95ZI4ogJYW9sCj5RbNJI5Ku00qkq3U_no8k81WIKGK62hiOR5n_EW2PwO8l0VotBQYaOQq4GWlA8MrE2hCJ6rQUcXRV_lO5eSCf7oUlysgh14YUqIlSa1P4v9jF4g-urXvnrug-YljYd2MdPEI1oQbW-kgUXa2zB44VqmeJ1UELiAPnTv3iXGRqWhvR6Z74KYPO4dP4OtSYV9t8mM87-y4-HuHy_Hhb7QJGwskyvZ613kKK1g_g_VsGAD3HNpp8xtn7KAvMG9ZUzFCiyz71jiSg-JPh-zMd5qw077QFlnf9lsRrGW-FoGZml3V7MtVd90wN3Zt5qScNHPamzmnndF1xvYdYUU_2-kFXBwenGeTYDGjITCJiLogSlGn9AtphC0qlKmtJJchilQmVUKxryA8HGlDoClJlJKlRWttmdoydtzyvExewmrd1LgFTJdlQbdBjI4xPuW0VZnUKhtirEJt7Qg-kMnyxTfW5j59Hke5XxzsmC_sOILxcJj5r563438P7A5HnpOdXdrE1Ej2yCOl6CpOZBqP4FXvC0uRsXQE_WHy-iG6vYP1yfnJcX58NP38Bh4TBJO-qo1vw2p3Pccdgjmdfet9-gZhXvqQ
linkToPdf http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwnV1ba9VAEB60gvri_XK8ruCTkNNc9pJ9LGkP9XYQa6XgQ9hNdrHtMSlNjqC_3plNckChiL6GzbCZnZ35wsx8A_BSVrHRUrhIO64iXnsdGe5NpBGdqEonnrtQ5buU-4f8zZE4GqsqqRcGN9GhpC4k8elWn9V-ZBhItun5SeAvaL-5ubA0J11chisCPS6Vc-0UB5sMAjFLDVypIqKgPHXvXCSGolPV_R6dLoCcIfQsbsKXzaZDxcnpfN3befXzDz7H__uqW3BjRKRsZzCh23DJNXfgWjENgrsL3bL97lZsbyg071jrGaJGVnxtieyg-tE7dhA6TtjHoeDWsaH91yO8ZaEmgZmGHTfs83F_3jIav7YiKe_bNa4tyHhX6NbYLhFXDDOe7sHhYu9TsR-Nsxoik4mkj5Lc6Rx_JY2wlXcyt15yGTuRy8xnGAMrxMWJNgieskwpWVtnra1zW6fEMc_r7D5sNW3jHgLTdV2hV0gdMcfnHJcqk1tlY5eqWFs7g1eosnK8a10Z0uhpUoaHkx7LUY8zmE8HWp4N_B1_e-HFdOwl6pnSJ6ZxqI8yUQpdcibzdAYPBnvYiEwlEfXH2aN_2dtzuPphd1G-e718-xiuIxKTobiNP4Gt_nztniLa6e2zYNa_AG35_RM
openUrl ctx_ver=Z39.88-2004&ctx_enc=info%3Aofi%2Fenc%3AUTF-8&rfr_id=info%3Asid%2Fsummon.serialssolutions.com&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Ajournal&rft.genre=article&rft.atitle=Novel+Elements+of+the+Chondrocyte+Stress+Response+Identified+Using+an+in+Vitro+Model+of+Mouse+Cartilage+Degradation&rft.jtitle=Journal+of+proteome+research&rft.au=Wilson%2C+Richard&rft.au=Golub%2C+Suzanne+B&rft.au=Rowley%2C+Lynn&rft.au=Angelucci%2C+Constanza&rft.date=2016-03-04&rft.pub=American+Chemical+Society&rft.issn=1535-3893&rft.eissn=1535-3907&rft.volume=15&rft.issue=3&rft.spage=1033&rft.epage=1050&rft_id=info:doi/10.1021%2Facs.jproteome.5b01115&rft.externalDocID=b002698663
thumbnail_l http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/lc.gif&issn=1535-3893&client=summon
thumbnail_m http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/mc.gif&issn=1535-3893&client=summon
thumbnail_s http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/sc.gif&issn=1535-3893&client=summon