Interaction of Ascorbate Peroxidase with Substrates:  A Mechanistic and Structural Analysis

• Published in: Biochemistry (Easton) Vol. 45; no. 25; pp. 7808 - 7817
• Main Authors: Macdonald, Isabel K, Badyal, Sandip K, Ghamsari, Lila, Moody, Peter C. E, Raven, Emma Lloyd
• Format: Journal Article
• Language: English
• Published: United States American Chemical Society 27.06.2006
• Subjects: Amino Acid Sequence; Ascorbate Peroxidases; Binding Sites; Crystallization; Crystallography, X-Ray; Hydrogen Bonding; Kinetics; Models, Chemical; Molecular Sequence Data; Oxidation-Reduction; Peroxidases - chemistry; Peroxidases - metabolism

Previous work [Sharp, K. H., et al. (2003) Nat. Struct. Biol. 10, 303−307] has revealed the location of the ascorbate binding site in ascorbate peroxidase and has identified hydrogen-bonding interactions to Arg172, Lys30, and the heme 6-propionate as important in formation of the enzyme−substrate co...