Incorporation of Sulfide Ions into the Cadmium(II) Thiolate Cluster of Cicer arietinum Metallothionein2

The plant metallothionein2 from Cicer arietinum (chickpea), cic-MT2, is known to coordinate five divalent metal ions such as ZnII or CdII, which are arranged in a single metal thiolate cluster. When the ZnII form of the protein is titrated with CdII ions in the presence of sulfide ions, an increased...

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Published inInorganic chemistry Vol. 52; no. 2; pp. 785 - 792
Main Authors Wan, Xiaoqiong, Freisinger, Eva
Format Journal Article
LanguageEnglish
Published United States American Chemical Society 18.01.2013
Subjects
Cadmium - chemistry
Cicer
Circular Dichroism
Coordination Complexes - chemistry
Coordination Complexes - metabolism
Escherichia - genetics
Escherichia - metabolism
Hydrogen-Ion Concentration
Ions
Metallothionein - chemistry
Metallothionein - genetics
Metallothionein - metabolism
Recombinant Proteins - genetics
Recombinant Proteins - metabolism
Spectrometry, Mass, Electrospray Ionization
Sulfhydryl Compounds - chemistry
Sulfides - chemistry
Zinc - chemistry
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Abstract The plant metallothionein2 from Cicer arietinum (chickpea), cic-MT2, is known to coordinate five divalent metal ions such as ZnII or CdII, which are arranged in a single metal thiolate cluster. When the ZnII form of the protein is titrated with CdII ions in the presence of sulfide ions, an increased CdII binding capacity and concomitant incorporation of sulfide ions into the cluster are observed. The exact stoichiometry of this novel cluster, its spectroscopic properties, and the significantly increased pH stability are analyzed with different techniques, including UV and circular dichroism spectroscopy and colorimetric assays. Limited proteolytic digestion provides information about the spacial arrangement of the cluster within the protein. Increasing the CdII scavenging properties of a metallothionein by additionally recruiting sulfide ions might be an economic and very efficient detoxification strategy for plants.
AbstractList The plant metallothionein2 from Cicer arietinum (chickpea), cic-MT2, is known to coordinate five divalent metal ions such as Zn(II) or Cd(II), which are arranged in a single metal thiolate cluster. When the Zn(II) form of the protein is titrated with Cd(II) ions in the presence of sulfide ions, an increased Cd(II) binding capacity and concomitant incorporation of sulfide ions into the cluster are observed. The exact stoichiometry of this novel cluster, its spectroscopic properties, and the significantly increased pH stability are analyzed with different techniques, including UV and circular dichroism spectroscopy and colorimetric assays. Limited proteolytic digestion provides information about the spacial arrangement of the cluster within the protein. Increasing the Cd(II) scavenging properties of a metallothionein by additionally recruiting sulfide ions might be an economic and very efficient detoxification strategy for plants.
The plant metallothionein2 from Cicer arietinum (chickpea), cic-MT2, is known to coordinate five divalent metal ions such as ZnII or CdII, which are arranged in a single metal thiolate cluster. When the ZnII form of the protein is titrated with CdII ions in the presence of sulfide ions, an increased CdII binding capacity and concomitant incorporation of sulfide ions into the cluster are observed. The exact stoichiometry of this novel cluster, its spectroscopic properties, and the significantly increased pH stability are analyzed with different techniques, including UV and circular dichroism spectroscopy and colorimetric assays. Limited proteolytic digestion provides information about the spacial arrangement of the cluster within the protein. Increasing the CdII scavenging properties of a metallothionein by additionally recruiting sulfide ions might be an economic and very efficient detoxification strategy for plants.
Author Wan, Xiaoqiong
Freisinger, Eva
AuthorAffiliation Institute of Inorganic Chemistry
Huazhong Agricultural University
University of Zurich
Ministry of Agriculture
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Snippet The plant metallothionein2 from Cicer arietinum (chickpea), cic-MT2, is known to coordinate five divalent metal ions such as ZnII or CdII, which are arranged...
The plant metallothionein2 from Cicer arietinum (chickpea), cic-MT2, is known to coordinate five divalent metal ions such as Zn(II) or Cd(II), which are...
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SubjectTerms Cadmium - chemistry
Cicer
Circular Dichroism
Coordination Complexes - chemistry
Coordination Complexes - metabolism
Escherichia - genetics
Escherichia - metabolism
Hydrogen-Ion Concentration
Ions
Metallothionein - chemistry
Metallothionein - genetics
Metallothionein - metabolism
Recombinant Proteins - genetics
Recombinant Proteins - metabolism
Spectrometry, Mass, Electrospray Ionization
Sulfhydryl Compounds - chemistry
Sulfides - chemistry
Zinc - chemistry
Title Incorporation of Sulfide Ions into the Cadmium(II) Thiolate Cluster of Cicer arietinum Metallothionein2
URI http://dx.doi.org/10.1021/ic301907j
https://www.ncbi.nlm.nih.gov/pubmed/23272701
https://search.proquest.com/docview/1273655557
Volume 52
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