Intrinsic Fluorescence Study of the Interaction of Human Apolipoprotein H with Phospholipid Vesicles

• Published in: Biochemistry (Easton) Vol. 38; no. 29; pp. 9477 - 9484
• Main Authors: Wang, Shao-Xiong, Cai, Guo-ping, Sui, Sen-fang
• Format: Journal Article
• Language: English
• Published: United States American Chemical Society 20.07.1999
• Subjects: Apolipoproteins - chemistry; Apolipoproteins - metabolism; beta 2-Glycoprotein I; Calcium - chemistry; Citraconic Anhydrides - chemistry; Disulfides - chemistry; Glycoproteins - chemistry; Glycoproteins - metabolism; Hot Temperature; Humans; Liposomes - chemistry; Liposomes - metabolism; Models, Chemical; Osmolar Concentration; Oxidation-Reduction; Phosphatidylglycerols - chemistry; Phosphatidylglycerols - metabolism; Phospholipids - chemistry; Phospholipids - metabolism; Protein Binding; Spectrometry, Fluorescence; Tryptophan - chemistry; Tryptophan - metabolism

Apolipoprotein H (ApoH) is a plasma glycoprotein with its in vivo physiological and pathogenic roles being closely related to its interaction with negatively charged membranes. In this paper, the interaction of ApoH with phospholipid vesicles was characterized by (i) detecting the wavelength shift o...