Thiophosphorylation of Histidine

• Published in: Journal of organic chemistry Vol. 65; no. 25; pp. 8448 - 8453
• Main Authors: Pirrung, Michael C, James, Kenneth D, Rana, Vipul S
• Format: Journal Article
• Language: English
• Published: WASHINGTON American Chemical Society 15.12.2000; Amer Chemical Soc
• Subjects: Chemistry; Chemistry, Organic; Chromatography, High Pressure Liquid; Histidine - chemistry; Kinetics; Magnetic Resonance Spectroscopy; Phosphorylation; Physical Sciences; Science & Technology; Spectrometry, Mass, Fast Atom Bombardment; Sulfhydryl Compounds - physiology; PHOSPHOHISTIDINE INTERMEDIATE; SIGNAL-TRANSDUCTION; PROTEIN; MECHANISM; PHOSPHOROTHIOATES; PHOSPHORYLATION; ESCHERICHIA-COLI; PHOSPHATE TRANSFER; NUCLEOSIDE DIPHOSPHATE KINASE; IDENTIFICATION

The preparation of a novel phosphorus species, thiophosphoramidate, has enabled the specific thiophosphorylation of histidine at its 3-position. The rates of phosphorylation and thiophosphorylation of histidine are reported, as well as the spectroscopic properties of both thiophosphoramidate and 3-t...