Kinetics and Mechanism of Long-Chain Fatty Acid Transport into Phosphatidylcholine Vesicles from Various Donor Systems

• Published in: Biochemistry (Easton) Vol. 41; no. 5; pp. 1591 - 1601
• Main Authors: Thomas, Richard M, Baici, Antonio, Werder, Moritz, Schulthess, Georg, Hauser, Helmut
• Format: Journal Article
• Language: English
• Published: United States American Chemical Society 05.02.2002
• Subjects: Arylsulfonates - chemistry; Biological Transport; Buffers; Carrier Proteins - chemistry; Carrier Proteins - metabolism; Fatty Acid Transport Proteins; Fatty Acids - metabolism; Fluorescent Dyes - chemistry; HEPES; Hydrogen-Ion Concentration; Kinetics; Lipid Bilayers - chemistry; Lipid Bilayers - metabolism; Macromolecular Substances; Membrane Proteins - chemistry; Membrane Proteins - metabolism; Membrane Transport Proteins; Oleic Acid - chemistry; Osmolar Concentration; Phosphatidylcholines - chemistry; Phosphatidylcholines - metabolism; Serum Albumin, Bovine - chemistry; Spectrometry, Fluorescence

The kinetics of long-chain fatty acid (FA) transfer from three different donor systems to unilamellar egg phosphatidylcholine (EPC) vesicles containing the pH-sensitive fluorophore pyranine in the vesicle cavity were determined. The transfer of long-chain FA from three FA donors, FA vesicles, unilam...