Conformation and Orientation of the Retinyl Chromophore in Rhodopsin:  A Critical Evaluation of Recent NMR Data on the Basis of Theoretical Calculations Results in a Minimum Energy Structure Consistent with All Experimental Data

• Published in: Biochemistry (Easton) Vol. 40; no. 14; pp. 4201 - 4204
• Main Authors: Singh, Deepak, Hudson, Bruce S, Middleton, Chris, Birge, Robert R
• Format: Journal Article
• Language: English
• Published: United States American Chemical Society 10.04.2001
• Subjects: Animals; Cattle; Crystallization; Crystallography, X-Ray; Deuterium; Isomerism; Nuclear Magnetic Resonance, Biomolecular - methods; Protein Conformation; Retinal Pigments - chemistry; Retinoids - chemistry; Rhodopsin - chemistry; Thermodynamics

In the absence of a high-resolution diffraction structure, the orientation and conformation of the protonated Schiffs base retinylidinium chromophore of rhodopsin within the opsin matrix has been the subject of much speculation. There have been two recent reliable and precise NMR results that bear o...