Resolution of Michaelis Complex, Acylation, and Conformational Change Steps in the Reactions of the Serpin, Plasminogen Activator Inhibitor-1, with Tissue Plasminogen Activator and Trypsin

• Published in: Biochemistry (Easton) Vol. 40; no. 39; pp. 11742 - 11756
• Main Authors: Olson, Steven T, Swanson, Richard, Day, Duane, Verhamme, Ingrid, Kvassman, Jan, Shore, Joseph D
• Format: Journal Article
• Language: English
• Published: United States American Chemical Society 02.10.2001
• Subjects: Acylation; Electrophoresis, Polyacrylamide Gel; Humans; Kinetics; Plasminogen Activator Inhibitor 1 - chemistry; Plasminogen Activator Inhibitor 1 - genetics; Plasminogen Activator Inhibitor 1 - metabolism; Protein Conformation; Serpins - chemistry; Serpins - metabolism; Spectrometry, Fluorescence; Tissue Plasminogen Activator - chemistry; Tissue Plasminogen Activator - metabolism; Trypsin - chemistry; Trypsin - metabolism

Michaelis complex, acylation, and conformational change steps were resolved in the reactions of the serpin, plasminogen activator inhibitor-1 (PAI-1), with tissue plasminogen activator (tPA) and trypsin by comparing the reactions of active and Ser 195-inactivated enzymes with site-specific fluoresce...