Observations of Tunable Resistive Pulse Sensing for Exosome Analysis: Improving System Sensitivity and Stability

Size distribution and concentration measurements of exosomes are essential when investigating their cellular function and uptake. Recently, a particle size distribution and concentration measurement platform known as tunable resistive pulse sensing (TRPS) has seen increased use for the characterizat...

Full description

Saved in:
Bibliographic Details
Published inLangmuir Vol. 31; no. 23; pp. 6577 - 6587
Main Authors Anderson, Will, Lane, Rebecca, Korbie, Darren, Trau, Matt
Format Journal Article
LanguageEnglish
Published United States American Chemical Society 16.06.2015
Subjects
Online AccessGet full text

Cover

Loading…
Abstract Size distribution and concentration measurements of exosomes are essential when investigating their cellular function and uptake. Recently, a particle size distribution and concentration measurement platform known as tunable resistive pulse sensing (TRPS) has seen increased use for the characterization of exosome samples. TRPS measures the brief increase in electrical resistance (a resistive pulse) produced by individual submicrometer/nanoscale particles as they translocate through a size-tunable submicrometer/micrometer-sized pore, embedded in an elastic membrane. Unfortunately, TRPS measurements are susceptible to issues surrounding system stability, where the pore can become blocked by particles, and sensitivity issues, where particles are too small to be detected against the background noise of the system. Herein, we provide a comprehensive analysis of the parameters involved in TRPS exosome measurements and demonstrate the ability to improve system sensitivity and stability by the optimization of system parameters. We also provide the first analysis of system noise, sensitivity cutoff limits, and accuracy with respect to exosome measurements and offer an explicit definition of system sensitivity that indicates the smallest particle diameter that can be detected within the noise of the trans-membrane current. A comparison of exosome size measurements from both TRPS and cryo-electron microscopy is also provided, finding that a significant number of smaller exosomes fell below the detection limit of the TRPS platform and offering one potential insight as to why there is such large variability in the exosome size distribution reported in the literature. We believe the observations reported here may assist others in improving TRPS measurements for exosome samples and other submicrometer biological and nonbiological particles.
AbstractList Size distribution and concentration measurements of exosomes are essential when investigating their cellular function and uptake. Recently, a particle size distribution and concentration measurement platform known as tunable resistive pulse sensing (TRPS) has seen increased use for the characterization of exosome samples. TRPS measures the brief increase in electrical resistance (a resistive pulse) produced by individual submicrometer/nanoscale particles as they translocate through a size-tunable submicrometer/micrometer-sized pore, embedded in an elastic membrane. Unfortunately, TRPS measurements are susceptible to issues surrounding system stability, where the pore can become blocked by particles, and sensitivity issues, where particles are too small to be detected against the background noise of the system. Herein, we provide a comprehensive analysis of the parameters involved in TRPS exosome measurements and demonstrate the ability to improve system sensitivity and stability by the optimization of system parameters. We also provide the first analysis of system noise, sensitivity cutoff limits, and accuracy with respect to exosome measurements and offer an explicit definition of system sensitivity that indicates the smallest particle diameter that can be detected within the noise of the trans-membrane current. A comparison of exosome size measurements from both TRPS and cryo-electron microscopy is also provided, finding that a significant number of smaller exosomes fell below the detection limit of the TRPS platform and offering one potential insight as to why there is such large variability in the exosome size distribution reported in the literature. We believe the observations reported here may assist others in improving TRPS measurements for exosome samples and other submicrometer biological and nonbiological particles.
Author Trau, Matt
Korbie, Darren
Lane, Rebecca
Anderson, Will
AuthorAffiliation Centre for Personalized NanoMedicine
The University of Queensland
Australian Institute for Bioengineering and Nanotechnology
School of Chemistry and Molecular Biosciences
AuthorAffiliation_xml – name: Australian Institute for Bioengineering and Nanotechnology
– name:
– name: Centre for Personalized NanoMedicine
– name: School of Chemistry and Molecular Biosciences
– name: The University of Queensland
Author_xml – sequence: 1
  givenname: Will
  surname: Anderson
  fullname: Anderson, Will
  email: will.anderson@uqconnect.edu.au
– sequence: 2
  givenname: Rebecca
  surname: Lane
  fullname: Lane, Rebecca
– sequence: 3
  givenname: Darren
  surname: Korbie
  fullname: Korbie, Darren
– sequence: 4
  givenname: Matt
  surname: Trau
  fullname: Trau, Matt
  email: m.trau@uq.edu.au
BackLink https://www.ncbi.nlm.nih.gov/pubmed/25970769$$D View this record in MEDLINE/PubMed
BookMark eNp9kMFO3DAQhq0KVBboG1SVj71kO3ac2O4NIdoiIVGxcI4c7xgZJfbWTlbs29erXThyskb-_n9G3zk5CTEgIV8ZLBlw9sPYvBxMeB5nn5ZND0wA_0QWrOFQNYrLE7IAKepKirY-I-c5vwCAroX-TM54oyXIVi_I5r7PmLZm8jFkGh19nIPpB6QPmH2e_Bbp33nISFcYsg_P1MVEb15jjiPSq2CGXcF-0ttxk-J2_7_a5QnHA17iftpRE9Z0NZneD2W6JKfOlMIvx_eCPP26ebz-U93d_769vrqrTC3UVElnrJTKcdUY1QqjuFBsrW1tOddGKo7InTVSWwcMrG4RuG6ZXFsLTvWiviDfD73lsH8z5qkbfbY4FGUY59yxVumWg9B1QcUBtSnmnNB1m-RHk3Ydg27vuiuuuzfX3dF1iX07bpj7EdfvoTe5BYADsI-_xDkVXfnjzv8CkJJv
CitedBy_id crossref_primary_10_1021_acsnano_0c10887
crossref_primary_10_1186_s40169_018_0192_7
crossref_primary_10_1002_ijc_30179
crossref_primary_10_3402_jev_v5_31242
crossref_primary_10_1016_j_xphs_2022_01_012
crossref_primary_10_3389_fmicb_2016_01500
crossref_primary_10_3389_fchem_2019_00396
crossref_primary_10_1186_s40486_022_00156_5
crossref_primary_10_2217_nnm_2016_0192
crossref_primary_10_3390_bioengineering6010007
crossref_primary_10_1016_j_jhazmat_2023_133014
crossref_primary_10_1021_acs_analchem_7b05106
crossref_primary_10_1016_j_jconrel_2020_10_020
crossref_primary_10_1111_prd_12520
crossref_primary_10_1002_jcp_25387
crossref_primary_10_1039_D0TB01499K
crossref_primary_10_1002_jev2_12052
crossref_primary_10_1016_j_biotechadv_2023_108122
crossref_primary_10_1021_acs_analchem_6b01818
crossref_primary_10_1021_acsanm_8b00267
crossref_primary_10_1016_j_bbagen_2016_07_028
crossref_primary_10_1002_advs_202401069
crossref_primary_10_1021_acssensors_9b01869
crossref_primary_10_1080_09537104_2016_1260704
crossref_primary_10_1016_j_chroma_2016_01_017
crossref_primary_10_1021_acs_chemrev_7b00777
crossref_primary_10_3390_nano11061481
crossref_primary_10_3390_ijms222312946
crossref_primary_10_1111_aji_13367
crossref_primary_10_1039_C8AN01270A
crossref_primary_10_1002_cbin_11620
crossref_primary_10_1016_j_vetpar_2016_03_008
crossref_primary_10_1063_5_0191456
crossref_primary_10_1007_s13233_016_4045_1
crossref_primary_10_3390_s21227645
crossref_primary_10_3390_bios13010129
crossref_primary_10_1021_acsami_7b18029
crossref_primary_10_1016_j_cytogfr_2023_08_003
crossref_primary_10_1016_j_biopha_2021_112270
crossref_primary_10_1016_j_nanoen_2021_106781
crossref_primary_10_1002_adbi_201700040
crossref_primary_10_1007_s10911_020_09473_0
crossref_primary_10_1021_acssensors_0c02728
crossref_primary_10_3390_vetsci11050187
crossref_primary_10_1155_2016_5741518
crossref_primary_10_1007_s10616_024_00631_4
crossref_primary_10_1016_j_bbagen_2018_07_016
crossref_primary_10_1021_acs_analchem_1c00693
crossref_primary_10_1039_C7NR07576F
crossref_primary_10_3390_diagnostics10110943
crossref_primary_10_1016_j_addr_2021_04_027
crossref_primary_10_1021_acsapm_0c01087
crossref_primary_10_1007_s00249_017_1252_4
crossref_primary_10_2217_nnm_2016_0097
crossref_primary_10_1016_j_jconhyd_2018_10_008
crossref_primary_10_1039_C7NR03998K
crossref_primary_10_1002_elan_202200091
crossref_primary_10_1016_j_cellsig_2024_111263
crossref_primary_10_1039_D0AY00170H
crossref_primary_10_1002_adhm_202202437
crossref_primary_10_3390_cells8040307
crossref_primary_10_1186_s13287_021_02697_9
crossref_primary_10_1016_j_bios_2020_112056
crossref_primary_10_1002_ppsc_202200130
crossref_primary_10_1016_j_sna_2022_113832
crossref_primary_10_1042_CS20220793
crossref_primary_10_1016_j_jchromb_2021_122604
crossref_primary_10_3390_mi9010020
crossref_primary_10_1002_elps_201900323
crossref_primary_10_3389_fimmu_2020_01509
crossref_primary_10_1002_advs_202103222
crossref_primary_10_1002_elps_202100202
crossref_primary_10_2174_1568026620666201019112557
crossref_primary_10_1016_j_ymeth_2020_02_003
crossref_primary_10_1021_acs_analchem_6b00796
crossref_primary_10_1002_elps_201800311
crossref_primary_10_1186_s12943_021_01312_y
crossref_primary_10_1109_ACCESS_2019_2907123
crossref_primary_10_1016_j_xphs_2021_07_012
crossref_primary_10_1016_j_ejpb_2021_12_003
crossref_primary_10_1016_j_jhip_2024_06_001
crossref_primary_10_1007_s12033_021_00300_3
crossref_primary_10_1063_5_0203512
crossref_primary_10_1016_j_bbamcr_2018_09_008
crossref_primary_10_1016_j_jddst_2022_103579
crossref_primary_10_1021_acsomega_8b03164
crossref_primary_10_3724_SP_J_1123_2021_07005
Cites_doi 10.1095/biolreprod.113.113266
10.1038/srep07639
10.1111/jth.12254
10.1016/j.jcis.2013.02.030
10.1038/nbt.1807
10.1063/1.3673596
10.1074/mcp.M111.012245
10.1016/j.nano.2011.04.003
10.1063/1.1684724
10.1021/ac200195n
10.1002/smll.201001129
10.1016/j.bbamcr.2013.03.013
10.1038/nm0598-594
10.1016/j.bios.2011.09.040
10.1016/j.colsurfb.2011.05.013
10.1016/j.addr.2012.07.002
10.1371/journal.pone.0082589
10.1002/smll.201200058
10.1002/ppsc.19910080134
10.1021/la2038763
10.1111/j.1538-7836.2010.04074.x
10.1016/j.transproceed.2013.08.079
10.1071/CH11200
10.1371/journal.pone.0067554
10.1097/QAD.0000000000000159
10.1002/cyto.a.20886
10.1016/j.ab.2012.06.004
10.1038/85438
10.1038/nprot.2012.065
10.1016/j.bios.2013.01.044
10.2217/nnm.12.173
10.1007/978-1-61779-068-3_15
10.1016/j.ymeth.2012.01.002
10.1021/la301351k
10.1038/ncb1596
10.1038/mt.2011.164
10.1155/2014/619829
10.3402/jev.v3.23784
ContentType Journal Article
Copyright Copyright © American Chemical Society
Copyright_xml – notice: Copyright © American Chemical Society
DBID CGR
CUY
CVF
ECM
EIF
NPM
AAYXX
CITATION
7X8
DOI 10.1021/acs.langmuir.5b01402
DatabaseName Medline
MEDLINE
MEDLINE (Ovid)
MEDLINE
MEDLINE
PubMed
CrossRef
MEDLINE - Academic
DatabaseTitle MEDLINE
Medline Complete
MEDLINE with Full Text
PubMed
MEDLINE (Ovid)
CrossRef
MEDLINE - Academic
DatabaseTitleList MEDLINE
MEDLINE - Academic

Database_xml – sequence: 1
  dbid: NPM
  name: PubMed
  url: https://proxy.k.utb.cz/login?url=http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed
  sourceTypes: Index Database
– sequence: 2
  dbid: EIF
  name: MEDLINE
  url: https://proxy.k.utb.cz/login?url=https://www.webofscience.com/wos/medline/basic-search
  sourceTypes: Index Database
DeliveryMethod fulltext_linktorsrc
Discipline Chemistry
EISSN 1520-5827
EndPage 6587
ExternalDocumentID 10_1021_acs_langmuir_5b01402
25970769
c559883635
Genre Research Support, Non-U.S. Gov't
Journal Article
GroupedDBID -
.K2
02
186
1WB
4.4
53G
55A
5GY
5VS
7~N
AABXI
ABDEX
ABFLS
ABMVS
ABPTK
ABUCX
ACGFS
ACJ
ACNCT
ACS
AEESW
AENEX
AFEFF
AFFNX
AGXLV
ALMA_UNASSIGNED_HOLDINGS
ANTXH
AQSVZ
BAANH
CS3
DU5
EBS
ED
ED~
EJD
F20
F5P
GNL
HR
IH9
IHE
JG
JG~
K2
LG6
OHM
RNS
ROL
TN5
UI2
UPT
VF5
VG9
W1F
X
---
-~X
AAHBH
ABJNI
ABQRX
ADHLV
AHGAQ
CGR
CUPRZ
CUY
CVF
ECM
EIF
GGK
NPM
YQT
~02
AAYXX
CITATION
7X8
ID FETCH-LOGICAL-a348t-7fac778f285a864a82481d9c3c229a782ee2fca79cf010c96e029617dcc0f8b43
IEDL.DBID ACS
ISSN 0743-7463
IngestDate Fri Aug 16 21:55:50 EDT 2024
Fri Dec 06 02:11:11 EST 2024
Sat Sep 28 08:13:29 EDT 2024
Thu Aug 27 13:42:35 EDT 2020
IsPeerReviewed true
IsScholarly true
Issue 23
Language English
LinkModel DirectLink
MergedId FETCHMERGED-LOGICAL-a348t-7fac778f285a864a82481d9c3c229a782ee2fca79cf010c96e029617dcc0f8b43
Notes ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
PMID 25970769
PQID 1689620493
PQPubID 23479
PageCount 11
ParticipantIDs proquest_miscellaneous_1689620493
crossref_primary_10_1021_acs_langmuir_5b01402
pubmed_primary_25970769
acs_journals_10_1021_acs_langmuir_5b01402
ProviderPackageCode JG~
55A
AABXI
GNL
VF5
7~N
ACJ
AGXLV
VG9
W1F
ANTXH
ACS
AEESW
AFEFF
.K2
ABMVS
ABUCX
IH9
1WB
BAANH
AQSVZ
ED~
UI2
PublicationCentury 2000
PublicationDate 2015-06-16
PublicationDateYYYYMMDD 2015-06-16
PublicationDate_xml – month: 06
  year: 2015
  text: 2015-06-16
  day: 16
PublicationDecade 2010
PublicationPlace United States
PublicationPlace_xml – name: United States
PublicationTitle Langmuir
PublicationTitleAlternate Langmuir
PublicationYear 2015
Publisher American Chemical Society
Publisher_xml – name: American Chemical Society
References Duijvesz D. (ref6/cit6) 2013; 8
Alvarez S. (ref5/cit5) 2013; 45
Dragovic R. A. (ref18/cit18) 2011; 7
Sun D. (ref3/cit3) 2013; 65
Anderson W. (ref21/cit21) 2013; 405
Valadi H. (ref2/cit2) 2007; 9
Sokolova V. (ref19/cit19) 2011; 87
Zitvogel L. (ref9/cit9) 1998; 4
Roberts G. S. (ref28/cit28) 2012; 31
van der Vlist E. J. (ref20/cit20) 2012; 7
Skogberg G. (ref41/cit41) 2013; 8
Gercel-Taylor C. (ref4/cit4) 2012; 428
Wolfers J. (ref8/cit8) 2001; 7
Dragovic R. A. (ref22/cit22) 2013; 89
Bell N. C. (ref31/cit31) 2012; 28
Taylor D. D. (ref42/cit42) 2011; 728
Low M. (ref27/cit27) 2011; 64
Vogel R. (ref29/cit29) 2011; 83
De Jaeger N. (ref34/cit34) 1991; 8
Roberts G. S. (ref25/cit25) 2010; 6
Willmott G. (ref23/cit23) 2012; 6
Maas S. L. N. (ref37/cit37) 2014; 92
Inder K. L. (ref40/cit40) 2012; 11
Naslund T. I. (ref7/cit7) 2014; 28
Boing A. N. (ref32/cit32) 2013; 1833
Lane R. E. (ref38/cit38) 2015; 5
Inder K. L. (ref39/cit39) 2014; 3
Roberts G. S. (ref36/cit36) 2012; 31
van der Pol E. (ref16/cit16) 2013; 11
Colombo M. (ref15/cit15) 2013; 126
Booth M. A. (ref24/cit24) 2013; 45
Franzen C. A. (ref30/cit30) 2014; 2014
Tauro B. J. (ref12/cit12) 2012; 56
Orozco A. F. (ref17/cit17) 2010; 77
Sharma S. (ref14/cit14) 2011; 27
van der Pol E. (ref1/cit1) 2010; 8
Platt M. (ref26/cit26) 2012; 8
Théry C. (ref13/cit13) 2001
de Vrij J. (ref33/cit33) 2013; 8
Alvarez-Erviti L. (ref10/cit10) 2011; 29
DeBlois R. W. (ref35/cit35) 1970; 41
Zhuang X. (ref11/cit11) 2011; 19
References_xml – volume: 89
  start-page: 151
  year: 2013
  ident: ref22/cit22
  publication-title: Biol. Reprod.
  doi: 10.1095/biolreprod.113.113266
  contributor:
    fullname: Dragovic R. A.
– volume: 5
  start-page: 7639
  year: 2015
  ident: ref38/cit38
  publication-title: Sci. Rep.
  doi: 10.1038/srep07639
  contributor:
    fullname: Lane R. E.
– volume: 11
  start-page: 36
  year: 2013
  ident: ref16/cit16
  publication-title: J. Thromb. Haemost.
  doi: 10.1111/jth.12254
  contributor:
    fullname: van der Pol E.
– volume: 405
  start-page: 322
  year: 2013
  ident: ref21/cit21
  publication-title: J. Colloid Interface Sci.
  doi: 10.1016/j.jcis.2013.02.030
  contributor:
    fullname: Anderson W.
– volume: 29
  start-page: 341
  year: 2011
  ident: ref10/cit10
  publication-title: Nat. Biotechnol.
  doi: 10.1038/nbt.1807
  contributor:
    fullname: Alvarez-Erviti L.
– volume: 6
  start-page: 014103
  year: 2012
  ident: ref23/cit23
  publication-title: Biomicrofluidics
  doi: 10.1063/1.3673596
  contributor:
    fullname: Willmott G.
– volume: 11
  start-page: M111 012245
  year: 2012
  ident: ref40/cit40
  publication-title: Mol. Cell Proteomics
  doi: 10.1074/mcp.M111.012245
  contributor:
    fullname: Inder K. L.
– volume: 7
  start-page: 780
  year: 2011
  ident: ref18/cit18
  publication-title: Nanomedicine
  doi: 10.1016/j.nano.2011.04.003
  contributor:
    fullname: Dragovic R. A.
– volume: 41
  start-page: 909
  year: 1970
  ident: ref35/cit35
  publication-title: Rev. Sci. Instrum.
  doi: 10.1063/1.1684724
  contributor:
    fullname: DeBlois R. W.
– volume: 83
  start-page: 3499
  year: 2011
  ident: ref29/cit29
  publication-title: Anal. Chem.
  doi: 10.1021/ac200195n
  contributor:
    fullname: Vogel R.
– volume: 126
  start-page: 5553
  year: 2013
  ident: ref15/cit15
  publication-title: J. Cell Sci.
  contributor:
    fullname: Colombo M.
– volume: 6
  start-page: 2653
  year: 2010
  ident: ref25/cit25
  publication-title: Small
  doi: 10.1002/smll.201001129
  contributor:
    fullname: Roberts G. S.
– volume: 1833
  start-page: 1844
  year: 2013
  ident: ref32/cit32
  publication-title: Biochim. Biophys. Acta
  doi: 10.1016/j.bbamcr.2013.03.013
  contributor:
    fullname: Boing A. N.
– volume: 4
  start-page: 594
  year: 1998
  ident: ref9/cit9
  publication-title: Nat. Med.
  doi: 10.1038/nm0598-594
  contributor:
    fullname: Zitvogel L.
– volume: 31
  start-page: 17
  year: 2012
  ident: ref28/cit28
  publication-title: Biosens. Bioelectron.
  doi: 10.1016/j.bios.2011.09.040
  contributor:
    fullname: Roberts G. S.
– volume: 87
  start-page: 146
  year: 2011
  ident: ref19/cit19
  publication-title: Colloids Surf., B
  doi: 10.1016/j.colsurfb.2011.05.013
  contributor:
    fullname: Sokolova V.
– volume: 65
  start-page: 342
  year: 2013
  ident: ref3/cit3
  publication-title: Adv. Drug Delivery Rev.
  doi: 10.1016/j.addr.2012.07.002
  contributor:
    fullname: Sun D.
– volume: 8
  start-page: e82589
  year: 2013
  ident: ref6/cit6
  publication-title: PLoS One
  doi: 10.1371/journal.pone.0082589
  contributor:
    fullname: Duijvesz D.
– volume: 8
  start-page: 2436
  year: 2012
  ident: ref26/cit26
  publication-title: Small
  doi: 10.1002/smll.201200058
  contributor:
    fullname: Platt M.
– volume-title: Current Protocols in Cell Biology
  year: 2001
  ident: ref13/cit13
  contributor:
    fullname: Théry C.
– volume: 8
  start-page: 179
  year: 1991
  ident: ref34/cit34
  publication-title: Part. Part. Syst. Charact.
  doi: 10.1002/ppsc.19910080134
  contributor:
    fullname: De Jaeger N.
– volume: 27
  start-page: 14394
  year: 2011
  ident: ref14/cit14
  publication-title: Langmuir
  doi: 10.1021/la2038763
  contributor:
    fullname: Sharma S.
– volume: 8
  start-page: 2596
  year: 2010
  ident: ref1/cit1
  publication-title: J. Thromb. Haemost.
  doi: 10.1111/j.1538-7836.2010.04074.x
  contributor:
    fullname: van der Pol E.
– volume: 45
  start-page: 3719
  year: 2013
  ident: ref5/cit5
  publication-title: Transplant Proc.
  doi: 10.1016/j.transproceed.2013.08.079
  contributor:
    fullname: Alvarez S.
– volume: 64
  start-page: 1229
  year: 2011
  ident: ref27/cit27
  publication-title: Aust. J. Chem.
  doi: 10.1071/CH11200
  contributor:
    fullname: Low M.
– volume: 8
  start-page: e67554
  year: 2013
  ident: ref41/cit41
  publication-title: PLoS One
  doi: 10.1371/journal.pone.0067554
  contributor:
    fullname: Skogberg G.
– volume: 28
  start-page: 171
  year: 2014
  ident: ref7/cit7
  publication-title: AIDS
  doi: 10.1097/QAD.0000000000000159
  contributor:
    fullname: Naslund T. I.
– volume: 77
  start-page: 502
  year: 2010
  ident: ref17/cit17
  publication-title: Cytometry A
  doi: 10.1002/cyto.a.20886
  contributor:
    fullname: Orozco A. F.
– volume: 31
  start-page: 17
  year: 2012
  ident: ref36/cit36
  publication-title: Biosen.s Bioelectron.
  doi: 10.1016/j.bios.2011.09.040
  contributor:
    fullname: Roberts G. S.
– volume: 428
  start-page: 44
  year: 2012
  ident: ref4/cit4
  publication-title: Anal. Biochem.
  doi: 10.1016/j.ab.2012.06.004
  contributor:
    fullname: Gercel-Taylor C.
– volume: 7
  start-page: 297
  year: 2001
  ident: ref8/cit8
  publication-title: Nat. Med.
  doi: 10.1038/85438
  contributor:
    fullname: Wolfers J.
– volume: 92
  start-page: e51623
  year: 2014
  ident: ref37/cit37
  publication-title: J. Vis. Exp.
  contributor:
    fullname: Maas S. L. N.
– volume: 7
  start-page: 1311
  year: 2012
  ident: ref20/cit20
  publication-title: Nat. Protoc.
  doi: 10.1038/nprot.2012.065
  contributor:
    fullname: van der Vlist E. J.
– volume: 45
  start-page: 136
  year: 2013
  ident: ref24/cit24
  publication-title: Biosens. Bioelectron.
  doi: 10.1016/j.bios.2013.01.044
  contributor:
    fullname: Booth M. A.
– volume: 8
  start-page: 1443
  year: 2013
  ident: ref33/cit33
  publication-title: Nanomedicine
  doi: 10.2217/nnm.12.173
  contributor:
    fullname: de Vrij J.
– volume: 728
  start-page: 235
  year: 2011
  ident: ref42/cit42
  publication-title: Methods Mol. Biol.
  doi: 10.1007/978-1-61779-068-3_15
  contributor:
    fullname: Taylor D. D.
– volume: 56
  start-page: 293
  year: 2012
  ident: ref12/cit12
  publication-title: Methods
  doi: 10.1016/j.ymeth.2012.01.002
  contributor:
    fullname: Tauro B. J.
– volume: 28
  start-page: 10860
  year: 2012
  ident: ref31/cit31
  publication-title: Langmuir
  doi: 10.1021/la301351k
  contributor:
    fullname: Bell N. C.
– volume: 9
  start-page: 654
  year: 2007
  ident: ref2/cit2
  publication-title: Nat. Cell Biol.
  doi: 10.1038/ncb1596
  contributor:
    fullname: Valadi H.
– volume: 19
  start-page: 1769
  year: 2011
  ident: ref11/cit11
  publication-title: Mol. Ther.
  doi: 10.1038/mt.2011.164
  contributor:
    fullname: Zhuang X.
– volume: 2014
  start-page: 619829
  year: 2014
  ident: ref30/cit30
  publication-title: Biomed Res. Int.
  doi: 10.1155/2014/619829
  contributor:
    fullname: Franzen C. A.
– volume: 3
  year: 2014
  ident: ref39/cit39
  publication-title: J. Extracell. Vesicles
  doi: 10.3402/jev.v3.23784
  contributor:
    fullname: Inder K. L.
SSID ssj0009349
Score 2.5249913
Snippet Size distribution and concentration measurements of exosomes are essential when investigating their cellular function and uptake. Recently, a particle size...
SourceID proquest
crossref
pubmed
acs
SourceType Aggregation Database
Index Database
Publisher
StartPage 6577
SubjectTerms Cryoelectron Microscopy
Electrochemical Techniques
Exosomes - ultrastructure
Membranes, Artificial
Nanopores
Organelle Size
Porosity
Sensitivity and Specificity
Title Observations of Tunable Resistive Pulse Sensing for Exosome Analysis: Improving System Sensitivity and Stability
URI http://dx.doi.org/10.1021/acs.langmuir.5b01402
https://www.ncbi.nlm.nih.gov/pubmed/25970769
https://search.proquest.com/docview/1689620493
Volume 31
hasFullText 1
inHoldings 1
isFullTextHit
isPrint
link http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwlV3dS8MwEA8yH_TF74_5RQRffOhc0zRtfJOxMQSdqIO9lSRNRGTtWFtR_3ov7TpREfW1hKS9u-Z-l7v7BaETxaXLJLU5dd84gIg17IOaOZQo4-o4IJrbbuSra9Yf0suRP_oIFL9m8Il7JlTWsmd34-Jx2vKljQhgy10kAUTgFgp17j5Idr0K7lrazYAyr26V-2EW65BU9tkh_YAyS2_TW0WDumenKjJ5ahW5bKm37xSOf_yQNbQyA574orKUdbSgkw201Knve9tEk4Gcn9BmODX4vij7qvCtzuxG8KzxTQGOFN_ZmvfkAQPcxd2XNEvHGtfcJud4fkqBKzL0anh1RwUWSYwB3pYFua9baNjr3nf6zuw-Bkd4NMydwAgVBKEhoS9CRkVIKKBdrjxFCBcANbQmRomAKwNRnuJMtwkHhBQr1TahpN42aiRponcRDmjsaSKYjEFZlBopvTYnIcRWvgG98iY6BXFFs_8pi8pUOXEj-7CWYTSTYRM5tQKjSUXR8cv441rLEYjYJkhEotMCVmEht_z83GuinUr98xkhTLQWx_f-8Wb7aBnwlW8ry1x2gBr5tNCHgGFyeVQa7jtSqe8I
link.rule.ids 314,780,784,2765,27076,27924,27925,56738,56788
linkProvider American Chemical Society
linkToHtml http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwlV1LT9wwEB4heoALFMpjgYKReuGQZeM4dsytWu1qebaCBXGLbMdGVbUJIhvU9td3nGwWUQlVXCPLmYwdzzeemW8AvhipQ66Zj6nHLkBEbPEctDxg1LjQZoJa6auRL6_46Jad3cf3CxC3tTAoRIkzlXUQ_4VdIDz2z_wV3qT68dSNtXcM8OT9EPuulR4R9W9euHajBvV69k3BeNRWzL0xi7dLpnxtl94Am7XRGa7C3VzcOtfkZ7ea6q758w-T47u_5yOszGAo-drsmzVYsPk6LPXb7m-f4PGbnt_XlqRwZFzVVVbk2pb-WHi25HuFZpXc-Az4_IEg-CWDX0VZTCxpmU5OyPzOgjTU6M3wpmMFUXlGEOzW6bm_N-B2OBj3R8GsO0OgIpZMA-GUESJxNIlVwplKKEPsK01kKJUKgYe11BklpHHo8xnJbY9KxEuZMT2XaBZtwmJe5HYbiGBZZKniOsM1Y8xpHfUkTdDTil1PcNmBI1RXOvu7yrQOnNMw9Q9bHaYzHXYgaNcxfWwIO_4z_rBd7BRV7MMlKrdFhW_hifRs_TLqwFazC-YzotMovGQ775DsAJZG48uL9OL06nwXlhF5xT7nLOR7sDh9quxnRDdTvV_v5b9fUfd1
linkToPdf http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwnV1ZSyQxEA6i4Pqyq67HrFeEffGhx-l0Oun4JqODt-IB4kuTpBORZboHe1rUX2-lj3EVRPQ1hHR1jqqvUpWvEPqrhfKZoi6mHloPELEBPWiYR4m2vkk4McK9Rj4-YXtX9OA6vP6v1BcIkcNIeRnEd6d6kNiaYcDfdO3uGq9f3N23Q-WcA9C-EyEoXJfNtd29eOXbDSrk6xg4OWVB82rug1GcbdL5W9v0AeAsDU_vF7oZiVzmm_xrF0PV1s_v2By_9U_T6GcNR_F2tX9m0JhJZ9GPblMF7jcanKrRvW2OM4svi_K1FT43uVMPDwafFWBe8YXLhE9vMYBgvPuY5Vnf4IbxZAuP7i5wRZFeda8qV2CZJhhAb5mm-zSHrnq7l909r67S4MmARkOPW6k5jyyJQhkxKiNCAQMLHWhChAQAYgyxWnKhLfh-WjDTIQJwU6J1x0aKBvNoPM1Ss4gwp0lgiGQqgXWj1CoVdASJwOMKbYcz0UIbMF1xfcryuAygEz92jc0cxvUctpDXrGU8qIg7Pum_3ix4DFPswiYyNVkBX2GRcKz9ImihhWonjEYE55E7yf58QbI1NHm204uP9k8Ol9AUALDQpZ75bBmND-8LswIgZ6hWy-38AlQc-fg
openUrl ctx_ver=Z39.88-2004&ctx_enc=info%3Aofi%2Fenc%3AUTF-8&rfr_id=info%3Asid%2Fsummon.serialssolutions.com&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Ajournal&rft.genre=article&rft.atitle=Observations+of+Tunable+Resistive+Pulse+Sensing+for+Exosome+Analysis%3A+Improving+System+Sensitivity+and+Stability&rft.jtitle=Langmuir&rft.au=Anderson%2C+Will&rft.au=Lane%2C+Rebecca&rft.au=Korbie%2C+Darren&rft.au=Trau%2C+Matt&rft.date=2015-06-16&rft.eissn=1520-5827&rft.volume=31&rft.issue=23&rft.spage=6577&rft_id=info:doi/10.1021%2Facs.langmuir.5b01402&rft_id=info%3Apmid%2F25970769&rft.externalDocID=25970769
thumbnail_l http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/lc.gif&issn=0743-7463&client=summon
thumbnail_m http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/mc.gif&issn=0743-7463&client=summon
thumbnail_s http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/sc.gif&issn=0743-7463&client=summon