Identification and Purification of Diphosphoinositol Pentakisphosphate Kinase, Which Synthesizes the Inositol Pyrophosphate Bis(diphospho)inositol Tetrakisphosphate

• Published in: Biochemistry (Easton) Vol. 37; no. 42; pp. 14998 - 15004
• Main Authors: Huang, Chang-Fen, Voglmaier, Susan M, Bembenek, Michael E, Saiardi, Adolfo, Snyder, Solomon H
• Format: Journal Article
• Language: English
• Published: United States American Chemical Society 20.10.1998
• Subjects: Animals; Brain - enzymology; Enzyme Activation; Hydrogen-Ion Concentration; Inositol Phosphates - biosynthesis; Kinetics; Phosphotransferases (Alcohol Group Acceptor) - chemistry; Phosphotransferases (Alcohol Group Acceptor) - isolation & purification; Phosphotransferases (Alcohol Group Acceptor) - metabolism; Phosphotransferases (Phosphate Group Acceptor) - metabolism; Proton-Translocating ATPases - metabolism; Rabbits; Rats

Diphosphoinositol pentakisphosphate (PP-IP5) and bis(diphospho)inositol tetrakisphosphate (bis-PP-IP4) were recently identified as inositol phosphates which possess pyrophosphate bonds. The molecular mechanisms that regulate the cellular levels of these compounds are not yet characterized. To pursue...