Dendronized Gold Nanoparticles as Carriers for gp160 (HIV-1) Peptides: Biophysical Insight into Complex Formation

The unavailability of effective and safe human immunodeficiency virus (HIV) vaccines incites several approaches for development of the efficient antigen/adjuvant vaccination composite. In this study, three different dendronized gold nanoparticles (AuNPs 13–15) were investigated for a complexation ab...

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Published inLangmuir Vol. 37; no. 4; pp. 1542 - 1550
Main Authors Garaiova, Zuzana, Melikishvili, Sopio, Michlewska, Sylwia, Ionov, Maksim, Pedziwiatr-Werbicka, Elzbieta, Waczulikova, Iveta, Hianik, Tibor, Gomez-Ramirez, Rafael, de la Mata, Francisco Javier, Bryszewska, Maria
Format Journal Article
LanguageEnglish
Published United States American Chemical Society 02.02.2021
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Abstract The unavailability of effective and safe human immunodeficiency virus (HIV) vaccines incites several approaches for development of the efficient antigen/adjuvant vaccination composite. In this study, three different dendronized gold nanoparticles (AuNPs 13–15) were investigated for a complexation ability with gp160 synthetic peptides derived from an HIV envelope. It has been shown that HIV peptides interacted with nanoparticles as evident from the changes in their secondary structures, restricted the mobility of the attached fluorescence dye, and enhanced peptide helicity confirmed by the fluorescence polarization and circular dichroism results. Transmission electron microscopy visualized complexes as cloud-like structures with attached nanoparticles. AuNP 13–15 nanoparticles bind negatively charged peptides depending on the number of functional groups; the fastest saturation and peptide retardation were observed for the most dendronized nanoparticle as indicated from dynamic light scattering, laser Doppler velocimetry, and agarose gel electrophoresis experiments. Dendronized gold nanoparticles can be considered one of the potential HIV peptide-based vaccination platforms.
AbstractList The unavailability of effective and safe human immunodeficiency virus (HIV) vaccines incites several approaches for development of the efficient antigen/adjuvant vaccination composite. In this study, three different dendronized gold nanoparticles (AuNPs 13-15) were investigated for a complexation ability with gp160 synthetic peptides derived from an HIV envelope. It has been shown that HIV peptides interacted with nanoparticles as evident from the changes in their secondary structures, restricted the mobility of the attached fluorescence dye, and enhanced peptide helicity confirmed by the fluorescence polarization and circular dichroism results. Transmission electron microscopy visualized complexes as cloud-like structures with attached nanoparticles. AuNP 13-15 nanoparticles bind negatively charged peptides depending on the number of functional groups; the fastest saturation and peptide retardation were observed for the most dendronized nanoparticle as indicated from dynamic light scattering, laser Doppler velocimetry, and agarose gel electrophoresis experiments. Dendronized gold nanoparticles can be considered one of the potential HIV peptide-based vaccination platforms.
Author Waczulikova, Iveta
Gomez-Ramirez, Rafael
de la Mata, Francisco Javier
Bryszewska, Maria
Ionov, Maksim
Hianik, Tibor
Melikishvili, Sopio
Garaiova, Zuzana
Michlewska, Sylwia
Pedziwiatr-Werbicka, Elzbieta
AuthorAffiliation Department of General Biophysics, Faculty of Biology and Environmental Protection
Department of Nuclear Physics and Biophysics, Faculty of Mathematics, Physics and Informatics
Laboratory of Microscopic Imaging and Specialized Biological Techniques, Faculty of Biology and Environmental Protection
University of Lodz
University Alcala
Networking Research Center on Bioengineering, Biomaterials and Nanomedicine (CIBER-BBN)
Ramón y Cajal Health Research Institute (IRYCIS)
Inorganic Chemistry Department, IQAR
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Title Dendronized Gold Nanoparticles as Carriers for gp160 (HIV-1) Peptides: Biophysical Insight into Complex Formation
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