Genome Mining of a Fungal Polyketide Synthase–Nonribosomal Peptide Synthetase Hybrid Megasynthetase Pathway to Synthesize a Phytotoxic N‑Acyl Amino Acid

• Published in: Organic letters Vol. 26; no. 17; pp. 3597 - 3601
• Main Authors: Chen, Lin, Wang, Xin, Zou, Yi, Tang, Man-Cheng
• Format: Journal Article
• Language: English
• Published: WASHINGTON American Chemical Society 03.05.2024; Amer Chemical Soc
• Subjects: Amino Acids - chemistry; Amino Acids - metabolism; Arabidopsis; Chemistry; Chemistry, Organic; Leucine - chemistry; Leucine - metabolism; Molecular Structure; Peptide Synthases - genetics; Peptide Synthases - metabolism; Physical Sciences; Plant Roots; Polyketide Synthases - metabolism; Science & Technology; PERCEPTION; BIOSYNTHESIS; OCHRATOXIN; DIVERSITY; IDENTIFICATION; METABOLITE; PSEUROTIN

Guided by the retrobiosynthesis hypothesis, we characterized a fungal polyketide synthase–nonribosomal peptide synthetase (PKS–NRPS) hybrid megasynthetase pathway to generate 2-trans-4-trans-2-methylsorbyl-d-leucine (1), a polyketide amino acid conjugate that inhibits Arabidopsis root growth. The bi...