Enrichment of (E)-Resveratrol from Peanut Byproduct with Molecularly Imprinted Polymers

• Published in: Journal of agricultural and food chemistry Vol. 59; no. 8; pp. 3539 - 3543
• Main Authors: Schwarz, Lachlan J, Danylec, Basil, Yang, Yuanzhong, Harris, Simon J, Boysen, Reinhard I, Hearn, Milton T. W
• Format: Journal Article
• Language: English
• Published: Washington, DC American Chemical Society 27.04.2011
• Subjects: Analytical Methods; Arachis - chemistry; Biological and medical sciences; Chromatography, High Pressure Liquid; Food industries; Fundamental and applied biological sciences. Psychology; Magnetic Resonance Spectroscopy; Polymers - chemistry; Solid Phase Microextraction; Spectrometry, Mass, Electrospray Ionization; Stilbenes - analysis; Tandem Mass Spectrometry; polyphenolics; peanut meal; resveratrol; solid phase extraction; procyanidins; molecularly imprinted polymers; Enrichment; Peanut meal; Peanut; Solid phase extraction; Polyphenol; Resveratrol; Polymer; Proanthocyanidin; Flavonoid
• DOI: 10.1021/jf104230f
• ISSN: 0021-8561

Molecularly imprinted solid phase extraction (MISPE) has been employed to isolate and concentrate bioactive polyphenols from peanut press waste. To this end, a molecularly imprinted polymer (MIP) templated with the phytoalexin (E)-resveratrol has been prepared via self-assembly with the functional monomer 4-vinylpyridine (4VP) in a 1:3 molar ratio. Subsequent molecular interrogation of the MIP binding sites demonstrated preferential structural selectivity for (E)-resveratrol with respect to other structurally related naturally occurring compounds. This selectivity was subsequently exploited to achieve substantial sample cleanup of peanut press waste under aqueous conditions with significant enrichment of (E)-resveratrol (>60 fold) requiring minimal sample preparation.