Development of a Candidate Reference Method for the Simultaneous Quantitation of the Boar Taint Compounds Androstenone, 3α-Androstenol, 3β-Androstenol, Skatole, and Indole in Pig Fat by Means of Stable Isotope Dilution Analysis–Headspace Solid-Phase Microextraction–Gas Chromatography/Mass Spectrometry

• Published in: Analytical chemistry (Washington) Vol. 83; no. 17; pp. 6785 - 6791
• Main Authors: Fischer, Jochen, Elsinghorst, Paul W, Bücking, Mark, Tholen, Ernst, Petersen, Brigitte, Wüst, Matthias
• Format: Journal Article
• Language: English
• Published: Washington, DC American Chemical Society 01.09.2011
• Subjects: Adipose Tissue - chemistry; Analytical chemistry; Androstenes - analysis; Androstenes - isolation & purification; Androstenols - analysis; Androstenols - isolation & purification; Animals; Calibration; Chemistry; Chromatographic methods and physical methods associated with chromatography; Deuterium - chemistry; Exact sciences and technology; Gas chromatographic methods; Gas Chromatography-Mass Spectrometry - methods; Gas Chromatography-Mass Spectrometry - standards; Indoles - analysis; Indoles - isolation & purification; Isotope Labeling; Other chromatographic methods; Skatole - analysis; Skatole - isolation & purification; Solid Phase Microextraction - methods; Solid Phase Microextraction - standards; Spectrometric and optical methods; Swine; Solid gas chromatography; Internal standard; Indole; Microanalysis; HPLC chromatography; Time; Freezing; Chemical enrichment; Degradation; Accuracy; Sample preparation; Injector; Detection limit; Extraction; Sampling; Quantitative analysis; Fluorescence detector; Sample; Isotope dilution; Tryptophan; Method; Contamination; Vaporization; Solid phase microextraction; Gas chromatography; Fat; Headspace; Feasibility; Mass spectrometry; Aromatic amine

The steroidal pig pheromones androstenone (5α-androst-16-en-3-one), 3α-androstenol (5α-androst-16-en-3α-ol), and 3β-androstenol (5α-androst-16-en-3β-ol) as well as the heterocyclic aromatic amines skatole and indole, originating from microbial degradation of tryptophan in the intestine of pigs, are...