Anti-CEA Antibody Fragments Labeled with [18F]AlF for PET Imaging of CEA-Expressing Tumors

• Published in: Bioconjugate chemistry Vol. 25; no. 2; pp. 335 - 341
• Main Authors: Lütje, S, Franssen, G. M, Sharkey, R. M, Laverman, P, Rossi, E. A, Goldenberg, D. M, Oyen, W. J. G, Boerman, O. C, McBride, W. J
• Format: Journal Article
• Language: English
• Published: WASHINGTON American Chemical Society 19.02.2014; Amer Chemical Soc
• Subjects: Aluminum Compounds; Animals; Biochemical Research Methods; Biochemistry & Molecular Biology; Carcinoembryonic Antigen - chemistry; Cell Line, Tumor; Cells; Chemistry; Chemistry, Multidisciplinary; Chemistry, Organic; Female; Fluorides; Fluorine Radioisotopes; Gene expression; Heterografts; Humans; Immunoglobulin Fragments - chemistry; Life Sciences & Biomedicine; Mice; Mice, Inbred BALB C; Mice, Nude; Neoplasms - diagnostic imaging; Physical Sciences; Positron-Emission Tomography; Rodents; Science & Technology; Tomography; Tumors; F-18; RESOLUTION; XENOGRAFTS

A facile and rapid method to label peptides with 18F based on chelation of [18F]AlF has been developed recently. Since this method requires heating to 100 °C, it cannot be used to label heat-sensitive proteins. Here, we used a two-step procedure to prepare 18F-labeled heat-labile proteins using the...