Ligand-Binding Cooperativity Effects in Polymer–Protein Conjugation

• Published in: Biomacromolecules Vol. 20; no. 2; pp. 1118 - 1131
• Main Authors: Reichenwallner, Jörg, Thomas, Anja, Steinbach, Tobias, Eisermann, Jana, Schmelzer, Christian E. H, Wurm, Frederik, Hinderberger, Dariush
• Format: Journal Article
• Language: English
• Published: United States American Chemical Society 11.02.2019
• Subjects: Binding Sites; Cyclic N-Oxides - chemistry; Drug Delivery Systems - methods; Dynamic Light Scattering - methods; Electron Spin Resonance Spectroscopy - methods; Ethylene Glycol - chemistry; Ligands; Methacrylates - chemistry; Polyethylene Glycols - chemistry; Polymers - chemistry; Serum Albumin, Bovine - chemistry

We present an electron paramagnetic resonance (EPR) spectroscopic characterization of structural and dynamic effects that stem from post-translational modifications of bovine serum albumin (BSA), an established model system for polymer–protein conjugation. Beyond the typical drug delivery and biocom...