Genetically Encoded Glutamate Indicators with Altered Color and Topology

• Published in: ACS chemical biology Vol. 13; no. 7; pp. 1832 - 1837
• Main Authors: Wu, Jiahui, Abdelfattah, Ahmed S, Zhou, Hang, Ruangkittisakul, Araya, Qian, Yong, Ballanyi, Klaus, Campbell, Robert E
• Format: Journal Article
• Language: English
• Published: United States American Chemical Society 20.07.2018

Glutamate is one of the 20 common amino acids and of utmost importance for chemically mediated synaptic transmission in nervous systems. To expand the color palette of genetically encoded indicators for glutamate, we used protein engineering to develop a red intensity-based glutamate-sensing fluores...