Evaluating Capture Sequence Performance for Single-Cell CRISPR Activation Experiments

• Published in: ACS synthetic biology Vol. 10; no. 3; pp. 640 - 645
• Main Authors: Choo, Xin Yi, Lim, Yu Ming, Katwadi, Khairunnisa, Yap, Lynn, Tryggvason, Karl, Sun, Alfred Xuyang, Li, Shang, Handoko, Lusy, Ouyang, John F, Rackham, Owen J. L
• Format: Journal Article
• Language: English
• Published: United States American Chemical Society 19.03.2021
• Subjects: Clustered Regularly Interspaced Short Palindromic Repeats - genetics; Human Embryonic Stem Cells; Humans; RNA, Guide, CRISPR-Cas Systems - metabolism; RNA, Messenger - metabolism; Single-Cell Analysis - methods; Transcription Factors - genetics; Transcription Factors - metabolism; feature barcoding technology; single-cell RNA-seq; CRISPR activation; overexpression; transcription factors; hESC

The combination of single-cell RNA sequencing with CRISPR inhibition/activation provides a high-throughput approach to simultaneously study the effects of hundreds if not thousands of gene perturbations in a single experiment. One recent development in CRISPR-based single-cell techniques introduces...