CsGOGAT Is Important in Dynamic Changes of Theanine Content in Postharvest Tea Plant Leaves under Different Temperature and Shading Spreadings

• Published in: Journal of agricultural and food chemistry Vol. 65; no. 44; pp. 9693 - 9702
• Main Authors: Liu, Zhi-Wei, Li, Hui, Wang, Wen-Li, Wu, Zhi-Jun, Cui, Xin, Zhuang, Jing
• Format: Journal Article
• Language: English
• Published: United States American Chemical Society 08.11.2017
• Subjects: Camellia sinensis - chemistry; Camellia sinensis - genetics; Camellia sinensis - growth & development; Camellia sinensis - metabolism; Food Handling; Gene Expression Regulation, Plant; Glutamates - analysis; Glutamates - biosynthesis; Plant Leaves - chemistry; Plant Leaves - genetics; Plant Leaves - growth & development; Plant Leaves - metabolism; Plant Proteins - genetics; Plant Proteins - metabolism; Temperature; postharvest leaves; tea plant; spreading; differentially expressed protein; CsGOGAT protein; gene expression; protein−protein interaction
• ISSN: 0021-8561; 1520-5118
• DOI: 10.1021/acs.jafc.7b04552

We analyzed the changes of theanine content in postharvest tea leaves under high temperature (38 °C), low temperature (4 °C), and shading spreadings by using ultrahigh-performance liquid chromatography. The differentially expressed proteins (DEPs), CsFd-GOGAT and CsNADH-GOGAT, which are involved in theanine biosynthesis pathway, were identified from the corresponding proteome data. The protein–protein interactions of CsFd-GOGAT and CsNADH-GOGAT, CsTS1, or CsNiR were verified by yeast two-hybrid technology. The expression profiles of 17 genes in theanine metabolism, including CsFd-GOGAT and CsNADH-GOGAT, were analyzed by quantitative real-time polymerase chain reaction. The correlations between the dynamic changes of theanine content and expression profiles of related genes and DEPs were analyzed. This study preliminarily proved the importance of CsGOGAT in dynamic changes of theanine content in postharvest tea leaves during spreading.