Reshaping the Binding Pocket of Cellobiose 2‑Epimerase for Improved Substrate Affinity and Isomerization Activity for Enabling Green Synthesis of Lactulose

• Published in: Journal of agricultural and food chemistry Vol. 70; no. 50; pp. 15879 - 15893
• Main Authors: Wang, Lu, Gu, Jiali, Zhao, Wei, Wang, Mingming, Ng, Kuan Rei, Lyu, Xiaomei, Yang, Ruijin
• Format: Journal Article
• Language: English
• Published: United States American Chemical Society 21.12.2022
• Subjects: Biotechnology and Biological Transformations; Cellobiose - metabolism; Isomerism; Lactose - chemistry; Lactulose - chemistry; Racemases and Epimerases - genetics; Racemases and Epimerases - metabolism; Substrate Specificity; binding region; cellobiose 2-epimerase; substrate affinity; isomerization activity; molecular dynamics simulation

Enzymatic isomerization of lactose into lactulose via cellobiose 2-epimerase (CE) could provide an eco-friendly route for the industrial production of lactulose, a valuable food prebiotic. However, poor substrate affinity for lactose and preference for epimerization over isomerization hinder this ap...