Characterization of a Novel Polysaccharide Derived from Rhizospheric Paecilomyces vaniformisi and Its Mechanism for Enhancing Salinity Resistance in Rice Seedlings

• Published in: Journal of agricultural and food chemistry Vol. 71; no. 51; pp. 20585 - 20601
• Main Authors: Ju, Xiu-yun, Gan, Shu, Yang, Ke-xin, Xu, Quan-bin, Dai, Wei-wei, Yangchen, Yongten Tsring, Zhang, Jie, Wang, Yue-nan, Li, Rong-peng, Yuan, Bo
• Format: Journal Article
• Language: English
• Published: United States American Chemical Society 27.12.2023
• Subjects: Agricultural and Environmental Chemistry; Oryza - metabolism; Paecilomyces; Polysaccharides - metabolism; Salinity; Seedlings; Soil - chemistry; Stress, Physiological; rhizospheric fungi; salt tolerance; antioxidant; polysacchairdes; rice seedling
• ISSN: 0021-8561; 1520-5118
• DOI: 10.1021/acs.jafc.3c05430

Soil salinity is an important limiting factor in agricultural production. Rhizospheric fungi can potentially enhance crop salinity tolerance, but the precise role of signaling substances is still to be systematically elucidated. A rhizospheric fungus identified as Paecilomyces vaniformisi was found to enhance the salinity tolerance of rice seedlings. In this study, a novel polysaccharide (PPL2b) was isolated from P. vaniformisi and identified as consisting of Manp, Glcp, GalpA, and Galp. In a further study, PPL2b showed significant activity in alleviating salinity stress-induced growth inhibition in rice seedlings. The results indicated that under salinity stress, PPL2b enhances seed germination, plant growth (height and biomass), and biochemical parameters (soluble sugar and protein contents). Additionally, PPL2b regulates genes such as SOS1 and SKOR to decrease K+ efflux and increase Na+ efflux. PPL2b increased the expression and activity of genes related to antioxidant enzymes and nonenzyme substances in salinity-induced oxidative stress. Further study indicated that PPL2b plays a crucial role in regulating osmotic substances, such as proline and betaine, in maintaining the osmotic balance. It also modulates plant hormones to promote rice seedling growth and enhance their tolerance to soil salinity. The variables interacted and were divided into two groups (PC1 77.39% and PC2 18.77%) based on their relative values. Therefore, these findings indicate that PPL2b from P. vaniformisi can alleviate the inhibitory effects of salinity stress on root development, osmotic adjustment, ion balance, oxidative stress balance, and growth of rice seedlings. Furthermore, it suggests that polysaccharides produced by rhizospheric fungi could be utilized to enhance crop tolerance to salinity.