Ion Mobility-Mass Spectrometry Coupled to Droplet Microfluidics for Rapid Protein Structure Analysis and Drug Discovery

• Published in: Analytical chemistry (Washington) Vol. 94; no. 38; pp. 13084 - 13091
• Main Authors: D’Amico, Cara I., Polasky, Daniel A., Steyer, Daniel J., Ruotolo, Brandon T., Kennedy, Robert T.
• Format: Journal Article
• Language: English
• Published: Washington American Chemical Society 27.09.2022
• Subjects: Algorithms; Analytical chemistry; Binders; Binding; Chemistry; Data acquisition; Data collection; Droplets; Dwell time; Dynamic structural analysis; Fingerprinting; Interpolation; Ionic mobility; Ions; Ligands; Mass spectrometry; Mass spectroscopy; Microfluidics; Mobility; Protein structure; Proteins; Scientific imaging; Spectroscopy; Workflow

Native mass spectrometry coupled to ion mobility (IM-MS) has become an important tool for the investigation of protein structure and dynamics upon ligand binding. Additionally, collisional activation or collision induced unfolding (CIU) can further probe conformational changes induced by ligand bind...