Label-Free Electrical Detection of Enzymatic Reactions in Nanochannels

• Published in: ACS nano Vol. 10; no. 8; pp. 7476 - 7484
• Main Authors: Duan, Chuanhua, Alibakhshi, Mohammad Amin, Kim, Dong-Kwon, Brown, Christopher M, Craik, Charles S, Majumdar, Arun
• Format: Journal Article
• Language: English
• Published: United States American Chemical Society 23.08.2016
• Subjects: Adsorption; Catalysis; Electricity; Enzymes - metabolism; Kinetics; Nanotechnology; Trypsin; trypsin proteolysis; surface enzymatic reactions; immobilized substrates; electrical detection; enzyme kinetics; nanochannels

We report label-free electrical detection of enzymatic reactions using 2-D nanofluidic channels and investigate reaction kinetics of enzymatic reactions on immobilized substrates in nanoscale-confined spaces. Trypsin proteolysis is chosen for demonstration of the detection scheme. When trypsin cleav...