Multifunctional Collagen and Hyaluronic Acid Multilayer Films on Live Mesenchymal Stem Cells

Cell encapsulation has been reported to convey cytoprotective effects and to better maintain cell survival. In contrast to other studies, our report shows that the deposition of two major biomacromolecules, collagen type I (Col) and hyaluronic acid (HA), on mesenchymal stem cells (MSCs) does not ent...

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Published inACS applied materials & interfaces Vol. 9; no. 14; pp. 12264 - 12271
Main Authors Choi, Daheui, Park, Jaeseong, Heo, Jiwoong, Oh, Tong In, Lee, EunAh, Hong, Jinkee
Format Journal Article
LanguageEnglish
Published United States American Chemical Society 12.04.2017
Subjects
Cell Differentiation
Cell Proliferation
Cells, Cultured
Collagen
Hyaluronic Acid
Mesenchymal Stem Cells
Osteogenesis
collagen
mutlifunctional nanofilm
mesenchymal stem cell
multilayer nanofilm
openness of film
hyaluronic acid
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Abstract Cell encapsulation has been reported to convey cytoprotective effects and to better maintain cell survival. In contrast to other studies, our report shows that the deposition of two major biomacromolecules, collagen type I (Col) and hyaluronic acid (HA), on mesenchymal stem cells (MSCs) does not entirely block the cell plasma membrane surface. Instead, a considerable amount of the surface remained uncovered or only slightly covered, as confirmed by TEM observation and by FACS analysis based on quantitative surface labeling. Despite this structure showing openness and flexibility, the multilayer Col/HA films significantly increased cell survival in the attachment-deprived culture condition. In terms of stem cell characteristics, the MSCs still showed functional cell activity after film deposition, as evidenced by their colony-forming activity and in vitro osteogenic differentiation. The Col/HA multilayer films could provide a cytoprotective effect and induce osteogenic differentiation without deteriorating effect or inhibition of cellular attachment, showing that this technique can be a valuable tool for modulating stem cell activities.
AbstractList Cell encapsulation has been reported to convey cytoprotective effects and to better maintain cell survival. In contrast to other studies, our report shows that the deposition of two major biomacromolecules, collagen type I (Col) and hyaluronic acid (HA), on mesenchymal stem cells (MSCs) does not entirely block the cell plasma membrane surface. Instead, a considerable amount of the surface remained uncovered or only slightly covered, as confirmed by TEM observation and by FACS analysis based on quantitative surface labeling. Despite this structure showing openness and flexibility, the multilayer Col/HA films significantly increased cell survival in the attachment-deprived culture condition. In terms of stem cell characteristics, the MSCs still showed functional cell activity after film deposition, as evidenced by their colony-forming activity and in vitro osteogenic differentiation. The Col/HA multilayer films could provide a cytoprotective effect and induce osteogenic differentiation without deteriorating effect or inhibition of cellular attachment, showing that this technique can be a valuable tool for modulating stem cell activities.
Author Oh, Tong In
Lee, EunAh
Park, Jaeseong
Hong, Jinkee
Choi, Daheui
Heo, Jiwoong
AuthorAffiliation Impedance Imaging Research Center
School of Chemical Engineering & Materials Science, College of Engineering
School of Medicine
Kyung Hee University
Chung-Ang University
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  surname: Oh
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  organization: Chung-Ang University
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Keywords collagen
mutlifunctional nanofilm
mesenchymal stem cell
multilayer nanofilm
openness of film
hyaluronic acid
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Snippet Cell encapsulation has been reported to convey cytoprotective effects and to better maintain cell survival. In contrast to other studies, our report shows that...
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SubjectTerms Cell Differentiation
Cell Proliferation
Cells, Cultured
Collagen
Hyaluronic Acid
Mesenchymal Stem Cells
Osteogenesis
Title Multifunctional Collagen and Hyaluronic Acid Multilayer Films on Live Mesenchymal Stem Cells
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