Palmitoylation of PD-L1 Regulates Its Membrane Orientation and Immune Evasion

• Published in: Langmuir Vol. 41; no. 8; pp. 5170 - 5178
• Main Authors: Zhang, Siya, Wang, Hong-Yin, Tao, Xuan, Chen, Zhongwen, Levental, Ilya, Lin, Xubo
• Format: Journal Article
• Language: English
• Published: United States American Chemical Society 04.03.2025
• Subjects: B7-H1 Antigen - chemistry; B7-H1 Antigen - immunology; B7-H1 Antigen - metabolism; Cell Line, Tumor; Cell Membrane - metabolism; energy transfer; fluorescence; Fluorescence Resonance Energy Transfer; Humans; Immune Evasion; lipids; Lipoylation; lung neoplasms; Membrane Microdomains - chemistry; Membrane Microdomains - metabolism; molecular dynamics; Molecular Dynamics Simulation; palmitoylation; plasma membrane; T-lymphocytes; therapeutics

Recently identified palmitoylation of PD-L1 is essential for immune regulation. To elucidate the underlying molecular mechanism, we performed giant plasma membrane vesicle (GPMV) experiments, μs-scale all-atom molecular dynamics (MD) simulations, fluorescence resonance energy transfer (FRET) experim...