Dissociation of Mannose-Rich Glycans Using Collision-Based and Electron-Based Ion Activation Methods
Three dissociation methods, including collision-induced dissociation (CID), electron capture dissociation (ECD), and electronic excitation dissociation (EED), were evaluated for the dissociation of doubly charged glycans using sodium or magnesium ions as charge carriers. CID produced mainly glycosid...
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Published in | Journal of the American Society for Mass Spectrometry Vol. 33; no. 5; pp. 803 - 812 |
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Main Authors | , , , , , |
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Language | English |
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American Chemical Society
04.05.2022
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Abstract | Three dissociation methods, including collision-induced dissociation (CID), electron capture dissociation (ECD), and electronic excitation dissociation (EED), were evaluated for the dissociation of doubly charged glycans using sodium or magnesium ions as charge carriers. CID produced mainly glycosidic cleavages, although more cross-ring fragment ions could be obtained at higher intensities when magnesium ions were used as charge carriers [M + Mg]2+. The 0,2A3, 0,3A3, and 0,4A3 ions provided structural information on the 3 → 1 and 6 → 1 linkages of the mannoses. Some internal fragment ions, such as 2,4A5_Y3β, were also produced in high abundance, thus providing additional information on the glycan structure. ECD produced limited fragments compared to other dissociation methods when either of the metal ions were used as charge carriers. Cross-ring fragments were obtained in relatively high abundance, with the charge mainly retained on the nonreducing end. EED produced extensive glycosidic and cross-ring cleavages when either metal charge carrier was used. A higher fragmentation efficiency was achieved and more structural-specific fragments were produced when Na+ was used as the charge carrier. Of the 31 possible cross-ring cleavages, including 0,2-, 0,4-, 1,5-, 2,4-, and 3,5-cleavages, 25 were found, thus providing extensive linkage information. A wide range of fragment ions could be obtained in all dissociation methods when Mg2+ was used as the charge carrier. Two specific analytical approaches were found to produce extensively structural-specific information on the glycans studied, namely CID of magnesiated glycans and EED of sodiated glycans. These two methods were selected to further analyze the larger mannose-rich glycans Man6GlcNAc2 and Man8GlcNAc2 and generated extensive structural information. |
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AbstractList | Three dissociation methods, including collision-induced dissociation (CID), electron capture dissociation (ECD), and electronic excitation dissociation (EED), were evaluated for the dissociation of doubly charged glycans using sodium or magnesium ions as charge carriers. CID produced mainly glycosidic cleavages, although more cross-ring fragment ions could be obtained at higher intensities when magnesium ions were used as charge carriers [M + Mg]
. The
A
,
A
, and
A
ions provided structural information on the 3 → 1 and 6 → 1 linkages of the mannoses. Some internal fragment ions, such as
A
_Y
, were also produced in high abundance, thus providing additional information on the glycan structure. ECD produced limited fragments compared to other dissociation methods when either of the metal ions were used as charge carriers. Cross-ring fragments were obtained in relatively high abundance, with the charge mainly retained on the nonreducing end. EED produced extensive glycosidic and cross-ring cleavages when either metal charge carrier was used. A higher fragmentation efficiency was achieved and more structural-specific fragments were produced when Na
was used as the charge carrier. Of the 31 possible cross-ring cleavages, including 0,2-, 0,4-, 1,5-, 2,4-, and 3,5-cleavages, 25 were found, thus providing extensive linkage information. A wide range of fragment ions could be obtained in all dissociation methods when Mg
was used as the charge carrier. Two specific analytical approaches were found to produce extensively structural-specific information on the glycans studied, namely CID of magnesiated glycans and EED of sodiated glycans. These two methods were selected to further analyze the larger mannose-rich glycans Man
GlcNAc
and Man
GlcNAc
and generated extensive structural information. Three dissociation methods, including collision-induced dissociation (CID), electron capture dissociation (ECD), and electronic excitation dissociation (EED), were evaluated for the dissociation of doubly charged glycans using sodium or magnesium ions as charge carriers. CID produced mainly glycosidic cleavages, although more cross-ring fragment ions could be obtained at higher intensities when magnesium ions were used as charge carriers [M + Mg]2+. The 0,2A3, 0,3A3, and 0,4A3 ions provided structural information on the 3 → 1 and 6 → 1 linkages of the mannoses. Some internal fragment ions, such as 2,4A5_Y3β, were also produced in high abundance, thus providing additional information on the glycan structure. ECD produced limited fragments compared to other dissociation methods when either of the metal ions were used as charge carriers. Cross-ring fragments were obtained in relatively high abundance, with the charge mainly retained on the nonreducing end. EED produced extensive glycosidic and cross-ring cleavages when either metal charge carrier was used. A higher fragmentation efficiency was achieved and more structural-specific fragments were produced when Na+ was used as the charge carrier. Of the 31 possible cross-ring cleavages, including 0,2-, 0,4-, 1,5-, 2,4-, and 3,5-cleavages, 25 were found, thus providing extensive linkage information. A wide range of fragment ions could be obtained in all dissociation methods when Mg2+ was used as the charge carrier. Two specific analytical approaches were found to produce extensively structural-specific information on the glycans studied, namely CID of magnesiated glycans and EED of sodiated glycans. These two methods were selected to further analyze the larger mannose-rich glycans Man6GlcNAc2 and Man8GlcNAc2 and generated extensive structural information. |
Author | Chan, T.-W. Dominic Wu, Ri Wong, H.-T. Kitty Wong, Y.-L. Elaine Chen, Xiangfeng Hung, Y.-L. Winnie |
AuthorAffiliation | Department of Chemistry Qilu University of Technology (Shandong Academy of Sciences) Shandong Analysis and Test Centre, School of Pharmaceutical Science |
AuthorAffiliation_xml | – name: Shandong Analysis and Test Centre, School of Pharmaceutical Science – name: Qilu University of Technology (Shandong Academy of Sciences) – name: Department of Chemistry |
Author_xml | – sequence: 1 givenname: H.-T. Kitty surname: Wong fullname: Wong, H.-T. Kitty organization: Department of Chemistry – sequence: 2 givenname: Xiangfeng orcidid: 0000-0001-9266-7707 surname: Chen fullname: Chen, Xiangfeng email: xiangfchensdas@163.com organization: Qilu University of Technology (Shandong Academy of Sciences) – sequence: 3 givenname: Ri surname: Wu fullname: Wu, Ri organization: Department of Chemistry – sequence: 4 givenname: Y.-L. Elaine surname: Wong fullname: Wong, Y.-L. Elaine organization: Department of Chemistry – sequence: 5 givenname: Y.-L. Winnie surname: Hung fullname: Hung, Y.-L. Winnie organization: Department of Chemistry – sequence: 6 givenname: T.-W. Dominic surname: Chan fullname: Chan, T.-W. Dominic email: twdchan@cuhk.edu.hk organization: Department of Chemistry |
BackLink | https://www.ncbi.nlm.nih.gov/pubmed/35380839$$D View this record in MEDLINE/PubMed |
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Snippet | Three dissociation methods, including collision-induced dissociation (CID), electron capture dissociation (ECD), and electronic excitation dissociation (EED),... |
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SubjectTerms | Electrons Humans Ions - chemistry Magnesium Mannose Polysaccharides - chemistry Sodium - chemistry |
Title | Dissociation of Mannose-Rich Glycans Using Collision-Based and Electron-Based Ion Activation Methods |
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