Enzymatic Modification of a Chemisorbed Lipid Monolayer

• Published in: Langmuir Vol. 12; no. 18; pp. 4411 - 4416
• Main Authors: Turner, David C, Peek, Brian M, Wertz, Thomas E, Archibald, Douglas D, Geer, Robert E, Gaber, Bruce Paul
• Format: Journal Article
• Language: English
• Published: Washington, DC American Chemical Society 04.09.1996
• Subjects: Biological and medical sciences; Biotechnology; Chemical industry. Chemical engineering; Fundamental and applied biological sciences. Psychology; Industrial applications and implications. Economical aspects; Monolayer; Enzymatic treatment; Chemical modification; Enzyme; Phospholipid; Hydrolases; Structure modification; Esterases; Phosphoric diester hydrolases; Phospholipase C; Surface structure

The selectivity and specificity of enzymes may be exploited to create chemically complex surfaces which are difficult or impossible to achieve using classical synthetic chemistry. In this paper we discuss the preparation of a chemisorbed lipid film on a silicon wafer and explore the activity of free...