Quantitative Profiling of Protein O‑GlcNAcylation Sites by an Isotope-Tagged Cleavable Linker

• Published in: ACS chemical biology Vol. 13; no. 8; pp. 1983 - 1989
• Main Authors: Qin, Ke, Zhu, Yuntao, Qin, Wei, Gao, Jinjun, Shao, Xuan, Wang, Yan-ling, Zhou, Wen, Wang, Chu, Chen, Xing
• Format: Journal Article
• Language: English
• Published: United States American Chemical Society 17.08.2018

Large-scale quantification of protein O-linked β-N-acetylglucosamine (O-GlcNAc) modification in a site-specific manner remains a key challenge in studying O-GlcNAc biology. Herein, we developed an isotope-tagged cleavable linker (isoTCL) strategy, which enabled isotopic labeling of O-GlcNAc through...